Method for preparing astragalus polysaccharides
A technology of astragalus polysaccharides and astragalus, which is applied in the field of preparation of astragalus polysaccharides, can solve the problems of low yield and content, destruction of astragalus polysaccharides, and increased costs, and achieve the effects of increasing yield and content, wide molecular weight distribution, and reducing production costs
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[0025] The preparation method of astragalus polysaccharide of the present invention comprises the following steps:
[0026] A, extract Astragalus with water to obtain water infusion;
[0027] B. Heating the water immersion liquid, discarding the bottom sediment after cooling to obtain the supernatant;
[0028] C. Concentrating the supernatant obtained in step B to a paste, adding ethanol for precipitation, and filtering to obtain the crude product of astragalus polysaccharide;
[0029] D. Refining the crude product of astragalus polysaccharide to obtain the refined product of astragalus polysaccharide.
[0030] The heating temperature in step B is 90-100° C., and the heating time is 15-20 minutes.
[0031] The extraction described in step A is diafiltration. Specifically, astragalus and water are added to the diafiltration column for circulatory diafiltration, the flow rate of the leachate is kept at 3-5ml / min, and the extraction time is 2-3 hours.
[0032] The dosage of As...
Embodiment 1
[0040] The preparation of embodiment 1 astragalus polysaccharide
[0041] The dried root of Astragalus membranaceus is washed, sliced, dried, pulverized by a pulverizer until passed through a 40-mesh sieve; weigh 100g of sieved dry powder of Astragalus membranaceus, add 250ml of water, stir and add it to the percolation column, adjust the pump speed to keep the leachate flow rate at 3ml / min, the extraction time was 3 hours, and the leachate was collected. Heat the obtained leachate in a water bath at 90°C for 15 minutes, let it stand to cool, and discard impurities such as denatured proteins in the bottom layer to precipitate. The supernatant was concentrated in a rotary evaporating dish under reduced pressure until it became a gelatinous paste, and then 3 times of 95% ethanol was added for precipitation to obtain 18.09 g of crude astragalus polysaccharide. The crude polysaccharide yield was 18.09%, and the polysaccharide content was 65.32%.
[0042] The crude astragalus po...
Embodiment 2
[0044] The preparation of embodiment 2 astragalus polysaccharides
[0045] Wash, slice and dry the dried roots of Astragalus membranaceus, crush them through a 60-mesh sieve; weigh 100g of sieved Astragalus membranaceus dry powder, add 250ml of water, stir and add to the percolation column, adjust the pump speed to keep the leachate flow rate at 4ml / min , the extraction time was 2.5 hours, and the leachate was collected. Heat the obtained leachate in a boiling water bath for 20 minutes, let it stand for cooling, then discard the denatured protein and other impurities in the bottom layer to precipitate. The supernatant was concentrated in a rotary evaporator under reduced pressure until it became a gelatinous paste, and then 3 times of 95% ethanol was added for precipitation to obtain 21.79 g of crude astragalus polysaccharide, with a crude polysaccharide yield of 21.79% and a polysaccharide content of 68.10%.
[0046] The crude astragalus polysaccharide is purified by ion excha...
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Description
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Application Information
- IPC
- C08B37/00; A61P9/10; A61P17/16; A61P31/16; A61P35/00; A61P37/04; A61P39/00; A61P39/06; A23K1/16; A23L1/09; A23K20/163; A23L33/125
- Inventors
- 丁海龙; 易彬