American ginseng compound base fertilizer
A composite bottom and American ginseng technology, applied in fertilization devices, fertilizer mixtures, applications, etc., can solve problems such as reduced soil absorption performance, imbalance of nutrient element ratios, and increased physical clay particles, so as to improve growth conditions, increase yields, and reduce The effect of disease occurrence
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Embodiment 1
[0046] The preparation of embodiment 1 saccharomyces cerevisiae inoculant
[0047] Saccharomyces cerevisiae (Saccharomyces cerevisiae) was purchased from China Agricultural Microorganism Culture Collection and Management Center, the strain number is ACCC 20040.
[0048] 1) Slant culture: Pick Saccharomyces cerevisiae cells and inoculate them on a fresh slope, and culture at 28°C for 24 hours;
[0049] 2) Erlenmeyer flask shaker culture: put the bacteria cultured in 1) into the Erlenmeyer flask liquid culture medium, shake and culture at 28°C and 200rpm for 15-20h;
[0050] 3) Fermentation culture: the bacterial solution obtained in 2) is inserted into the fermentation liquid medium at an inoculation amount of 2-3v / v% for fermentation, and cultured at 28° C. for 24 hours, and the fermentation aeration rate is 0.1-1;
[0051] 4) Separation of bacteria and preparation of bacterial agent: centrifuge the fermentation broth obtained in 3), wash the centrifuged bacteria with water f...
Embodiment 2
[0053] The preparation of embodiment 2 acidophilus lactic acid bacteria agent
[0054] Lactobacillus acidophilus (Lactobacillus acidophilus) was purchased from China Agricultural Microorganism Culture Collection and Management Center, the strain number is ACCC 10637.
[0055] 1) Activation culture: Inoculate acidophilus lactic acid bacteria stored in glycerol at -20°C on the slope of the activation medium, put them in a desiccator, react with pyrogallic acid and sodium hydroxide to remove oxygen, and then culture at 37°C for 2 days Afterwards, transfer to the liquid activation medium in the Erlenmeyer flask and culture at 37°C for 30h;
[0056] 2) Fermentation culture: the bacterium solution obtained in 1) is inserted into the fermentation liquid culture medium according to the inoculum amount of 10v / v% to carry out fermentation, feeds nitrogen gas to keep dissolved oxygen at 0, cultivates at 37 ℃ for 24 hours, and obtains live The number of bacteria ≥ 10 7 CFU / g of bacteria...
Embodiment 3
[0059] The preparation of embodiment 3 subtilis agent
[0060] Bacillus subtilis (Bacillus subtilis) was purchased from the China Agricultural Microorganism Culture Collection and Management Center, and the strain number is ACCC 02973.
[0061] 1) Slant culture: Pick Bacillus subtilis and inoculate it on a fresh slant, and culture at 37°C for 24 hours;
[0062] 2) Activation culture: put the bacteria cultured in 1) into the liquid activation medium in the Erlenmeyer flask, and culture at 37°C with shaking at 180r / min for 20h;
[0063] 3) Fermentation culture: transfer the bacterial solution obtained in 2) into the fermentation liquid medium for fermentation, and cultivate it at 38°C for 24 hours to obtain the number of viable bacteria ≥ 10 7 CFU / g of bacterial agent 3;
[0064] Wherein, the medium used in 1) and 2) is peptone 2g / L, yeast extract 0.3g / L and glucose, pH 7.2; 3) the fermentation medium described in is glucose 3g / L, yeast extract 1g / L, Peptone 1g / L, CaSO 4 1g / ...
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