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Method for quickly suppressing activity of acidic trehalose lytic enzyme in beer yeast cell

A technology of beer yeast cells and trehalose decomposing enzymes, which is applied in the field of rapidly inhibiting the activity of beer yeast cells acidic trehalose decomposing enzymes, can solve the problems such as the activity of beer yeast cells acidic trehalose decomposing enzymes, etc. The effect of sewage discharge and process route is simple

Inactive Publication Date: 2011-09-07
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] To sum up, looking at the relevant research and technical reports at home and abroad, there is no method to quickly inhibit the activity of acidic trehalose decomposing enzymes in brewer's yeast cells by using high-voltage pulsed electric field technology, and realize the acidic trehalose in brewer's yeast cells within 60 μs. A technological breakthrough with an inhibition rate of over 95% for decomposing enzyme activity

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0026]Using live yeast cells as raw materials, according to brewer's yeast cells: water = 1: 10g / mL, after mixing evenly, immediately place it at 5°C for mechanical stirring for 5 minutes, and use 0.1M hydrochloric acid solution and 01M hydrogen during the stirring process Sodium oxide solution is used to adjust the pH value of brewer's yeast cell suspension to 1.5; select a suitable electrode with a length of 0.5 mm and a radius of 0.5 mm, and then select 75% ethanol, deionized water, and acidic deionized water (using 0.1M hydrochloric acid solution and 0.1 M sodium hydroxide solution deployment pH value to 15) After cleaning the material circulation pipeline in the high-voltage pulse electric field for 2 times, then adjust and set the electric field intensity of the high-voltage pulse electric field device to be 45kV / cm and the frequency to be 3000Hz, and then keep the material constant The flow pump is adjusted so that the flow rate of the beer yeast cell suspension is 45mL / ...

Embodiment 2

[0028] Using beer yeast slime as raw material, the waste beer yeast slime is first prepared into 8% mixed liquid, passed through 80 mesh sieve twice, and then 5% tartaric acid is added, and after natural sedimentation for 30 minutes, low-temperature centrifugation is carried out (4°C, 3600r / min, 10min), discard the supernatant to obtain spent beer yeast cells; then mix evenly according to beer yeast cells: water = 1: 20g / mL, immediately place it under a temperature environment of 18°C ​​for magnetic stirring for 3min, and stir again In the process, 0.1M hydrochloric acid solution and 0.1M sodium hydroxide solution were used to adjust the pH value of the beer yeast cell suspension to 2.5; a suitable electrode with a length of 1.5mm and a radius of 0.5mm was selected, and then 75% ethanol, deionized water, and Acidic deionized water (use 0.1M hydrochloric acid solution and 0.1M sodium hydroxide solution to adjust the pH value to 2.5) to clean the material circulation pipeline in...

Embodiment 3

[0030] Using live yeast cells as raw materials, according to brewer's yeast cells: water = 1: 100g / mL, after mixing evenly, immediately place it at 10°C for magnetic stirring for 5 minutes, and use 0.1M hydrochloric acid solution and 0.1M hydrochloric acid solution during the stirring process. Sodium hydroxide solution is used to adjust the pH value of brewer's yeast cell suspension to 3.5; select a suitable electrode with a length of 3.5mm and a radius of 0.8mm, and then select 75% ethanol, deionized water, and acidic deionized water (using 0.1M hydrochloric acid solution) and 0.1M sodium hydroxide solution to adjust the pH value to 3.5) after cleaning the material circulation pipeline in the high-voltage pulse electric field for 3 times, then adjust the electric field intensity of the high-voltage pulse electric field device to 10kV / cm and the frequency to 2000Hz, and then The constant flow pump of the material is adjusted to the flow rate of the beer yeast cell suspension at...

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Abstract

The invention discloses a method for quickly suppressing activity of an acidic trehalose lytic enzyme in a beer yeast cell, belonging to the technical fields of fine and further processing of agricultural and sideline products and comprehensive utilization of byproducts of the agricultural and sideline products, particularly relating to a method for suppressing the activity of the acidic trehalose lytic enzyme in the beer yeast cell by using a high-voltage pulsed electric field technology. In the method, a high-voltage pulsed electric field device is used, electrodes with both proper length and diameter are selected and used; electric field strength and frequency in the high-voltage pulsed electric field device are controlled to be within proper ranges; and beer yeast cell suspension with proper concentration and proper pH value is pumped to the electrodes of the high-voltage pulsed electric field device at a certain constant flow speed for stream processing and then is collected in a finished product tank. The technical preparation method comprises the following steps of: preparing the beer yeast cell suspension; regulating acidity of the suspension; adjusting and controlling parameters of the high-voltage pulsed electric field; and adjusting and controlling constant flow pumping speed of the suspension. The method has the advantages that: the suppression ratio of the activity of the acidic trehalose lytic enzyme in the beer yeast cell reaches over 95 percent within 60 microseconds; a technical support is provided for production technology research of quick trehalose extraction; and a certain foundation can be laid for comprehensive development for changing waste beer yeast mud into things of value.

Description

technical field [0001] The invention belongs to the technical field of deep processing of agricultural and sideline products and comprehensive utilization of by-products thereof, and relates to a method for rapidly inhibiting activity of beer yeast cell acidic trehalose decomposing enzyme by using high-voltage pulse electric field technology. Background technique [0002] Trehalose has been identified as the sugar of life in the 21st century. At present, its extraction and separation technology and its application fields have been identified as hot issues and focus issues by domestic and foreign scholars. Trehalose is a non-reducing disaccharide consisting of two glucose molecules linked by glycosidic bonds. With the discovery and improvement of the special function of trehalose, which can protect biofilms, membrane proteins, nucleic acids, etc. under special environments such as freezing, drying, and high osmotic pressure, and the research on the properties of trehalose In...

Claims

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Application Information

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IPC IPC(8): C12N13/00C12R1/865
Inventor 林松毅王睦刘静波金艳张美硕刘明源杨艺宫新统殷涌光赫桂丹
Owner JILIN UNIV
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