Method for preparing phosphatidylcholine type omega-3 unsaturated fatty acid by using enzyme method

A technology of unsaturated fatty acid and phosphatidylcholine, which is applied in the field of enzymatic preparation of phosphatidylcholine type omega-3 unsaturated fatty acid, which can solve the problems of unfavorable industrialization, low reaction conversion rate, and inability to use food production, etc.

Inactive Publication Date: 2013-05-01
JIANGYIN BEC BIOSCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this regard, such as Chinese patent application 200710113934.6, a process for preparing phospholipids rich in polyunsaturated fatty acids is to use enzymes to catalyze fish oil omega-3 and soybean phospholipids to synthesize phospholipid-type omega-3, but the starting material soybean phospholipids of this patent is a A mixture of glycerides, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, serine phospholipids and phosphatidic acid. After the reaction, not only the phosphatidylcholine type omega-3 content is small, but also the reaction conversion rate of ester conversion is very low. What is even more unfavorable to industrialization is that the reaction medium R134a used in this patent is a chemical raw material and cannot be used for food production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Step 1, the preparation of high-purity seal oil free type omega-3 unsaturated fatty acid

[0023] Add 5.5 grams of seal oil and 5-6 mL of phosphate buffer solution with a pH of 7 into the reaction vessel, then add 1000 U / mL Aspergillus niger lipase, stir and enzymolyze at 40°C for 8 hours, and oxidize with 0.3 mol / L hydrogen Sodium ethanol solution to carry out phase separation, remove diglyceride, and obtain free fatty acid phase; take 4-6 g urea and 25-35 mL ethanol into a round bottom flask, put it in a 60-65 °C water bath, and stir until the solution is clear , quickly pour it into a beaker containing the free fatty acid phase, cool it to room temperature while stirring, place it in the refrigerator to crystallize at -35 degrees, and quickly filter the crystallization mixture to obtain two parts, the crystal clathrate and the filtrate, in which most saturated fatty acids Being included in the crystals, the polyunsaturated fatty acids in the filtrate are thus enriche...

Embodiment 2

[0027] Step 1, the preparation of high-purity seal oil free type omega-3 unsaturated fatty acid

[0028] Add 5 grams of seal oil and 5 mL of phosphate buffer solution with a pH of 8 into the reaction vessel, then add 800 U / mL Candida lipase, stir and enzymatically hydrolyze at 35°C for 10 hours, and use 0.25 mol / L sodium hydroxide Carry out phase separation with ethanol solution, remove diglyceride, and obtain free fatty acid phase; take 4-6 g urea and 25-35 mL ethanol into a round bottom flask, put it in a water bath at 60-65 °C, stir until the solution is clear, and quickly Pour it into a beaker containing the free fatty acid phase, cool to room temperature while stirring, place it in the refrigerator to crystallize at -35°C, and quickly filter the crystallization mixture to obtain two parts, the crystal clathrate and the filtrate, in which most saturated fatty acids are encapsulated Combined in the crystals, the polyunsaturated fatty acids in the filtrate are thus enriched....

Embodiment 3

[0032] Step 1, the preparation of high-purity seal oil free type omega-3 unsaturated fatty acids

[0033] Add 5 grams of seal oil and 5 mL of phosphate buffer solution with a pH of 8 into the reaction vessel, then add 900 U / mL Penicillium arcuatus lipase, stir and enzymatically hydrolyze at 35 °C for 9 hours, and oxidize with 0.3 mol / L hydrogen Sodium ethanol solution was used for phase separation to remove diglyceride and obtain free fatty acid phase; take 4-6 g of urea and 25-35 mL of ethanol into a round bottom flask, put it in a 60-65°C water bath, stir until the solution is clear, Quickly pour it into a beaker containing the free fatty acid phase, cool to room temperature while stirring, place it in the refrigerator to crystallize at -35°C, and quickly filter the crystallization mixture to obtain two parts, the crystal clathrate and the filtrate, in which most saturated fatty acids are Included in the crystals, the polyunsaturated fatty acids in the filtrate are thus enri...

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PUM

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Abstract

The invention relates to a method for preparing phosphatidylcholine type omega-3 unsaturated fatty acid by using an enzyme method. The method is characterized by comprising the following process steps of: 1, performing lipase selective enzymolysis, separation and purification to obtain high-purity seal oil free type omega-3 unsaturated fatty acid by taking seal oil triglyceride as a raw material;and 2, adding the high-purity seal oil free type omega-3 unsaturated fatty acid obtained in the step 1, serving as a raw material, into lysophosphatidyl choline to obtain a phosphatidylcholine type omega-3 unsaturated fatty product under the non-aqueous phase catalytic action of an enzyme. The method has the advantages of low cost and low pollution, and the prepared phosphatidylcholine type omega-3 unsaturated fatty acid has high purity and is rich in DPA (Docosaentaenoic Acid), EPA (Eicosapentaenoic Acid) and DHA (Docosahexaenoic Acid).

Description

technical field [0001] The invention relates to a method for preparing phosphatidylcholine type omega-3 unsaturated fatty acid by enzymatic method. Background technique [0002] Phosphatidylcholine is known as the "third nutrient" alongside protein and vitamins, and is listed as a daily essential nutrient by the Food and Nutrition Society of the National Academy of Sciences. Moreover, phosphatidylcholine has been clinically used in the treatment of diseases such as atherosclerosis, acute and chronic hepatitis, fatty liver, liver cirrhosis, and neurasthenia in the United States, Europe, and Japan. Omega-3 unsaturated fatty acids (abbreviated as Omega-3) have physiological functions such as promoting brain and vision development of infants, preventing cardiovascular diseases, lowering blood lipids, delaying aging, preventing senile dementia, and preventing cancer. In recent years, the dual role of omega-3 and phosphatidylcholine has become a hot topic in medical research and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/00
Inventor 雷继雨李建洲徐枫
Owner JIANGYIN BEC BIOSCI & TECH
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