Composite microbial agent for direct decomposition of crop straws returned to field and preparation method thereof
A technology of compound microorganisms and crop straws, applied in the field of microorganisms, can solve the problems of slow decomposition and difficulty in returning to the field
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Embodiment 1
[0085] A compound microbial preparation for quickly decomposing crop stalks, which is prepared from the following raw materials in weight percentage:
[0086] Raw material % Raw material %
[0087] Bacillus subtilis 29%; Saccharomyces cerevisiae 12%;
[0088] Aspergillus niger 11%; Trichoderma pseudoconii 20%;
[0089] Phanerochaete xanthophyte 18%; Streptomyces albicans 10%.
[0090] The Bacillus subtilis, Saccharomyces cerevisiae, Aspergillus niger, Trichoderma pseudoconii, Phanerochaete xanthophyllum, and Streptomyces albicans were all purchased from China Agricultural Microorganism Culture Collection and Management Center.
[0091] A preparation method for a compound microbial preparation for rapidly decomposing crop stalks, the steps of which are:
[0092] (1), separate expansion culture of various strains:
[0093] A. Cultivation of Bacillus subtilis: inoculate the test tube species of Bacillus subtilis on beef extract peptone medium (refer to microbiology test textb...
test example 1
[0104] The test site is in the second group of Jindang Village, Muzi Township, Dajin Town, Wuxue City. The soil used for the test is paddy soil developed from fluvo-aquic soil, and the previous stubble is early rice. In the rice-rice mode, after the early rice is harvested, all the straw is returned to the field, and no-tillage is implemented. 4 treatments were set up in the experiment, and the area of the plot was 20m 2 (4m×5m), ridges were built between plots, and the experiment was repeated 4 times. The plots were treated as follows: Treatment 1: Return full amount of straw to field + straw decomposing agent; Treatment 2: Return full amount of straw to field + equal amount of water; Treatment 3: Full amount Straw returning; treatment 4: no straw returning.
[0105] Analysis of test results
[0106] Color change of straw: On the third day after decomposing agent was applied, the color of the straw without decomposing agent basically remained unchanged, and the surface of t...
test example 2
[0109] The experimental field is located in the paddy field of Wuji Po Fu Zuyou farmers of Wuji Village Committee, Nada Town, Danzhou City. The geographical location of the field is: east diameter 109 degrees 3 minutes 39.03 seconds; north latitude 19 degrees 38 minutes 31.01 seconds; height 94 meters. The previous stubble was the early paddy field. The soil type is purple mud field. The soil parent material is purple sand shale. The physical and chemical properties of the 0-20cm soil before transplanting are as follows: organic matter 18.75g / kg; total nitrogen 1.01g / kg; available nitrogen 53.00mg / kg; available phosphorus 4.22mg / kg; available potassium 104mg / kg; pH value 5.8.
[0110] Test fertilizer: rice straw: 400 kg / mu; straw decomposing agent 2 kg / mu; large particle urea 20 kg / mu (produced by CNOOC Chemical Co., Ltd.); superphosphate (produced in Zhanjiang) 25 kg / mu; potassium chloride ( Canada) 15 kg / mu.
[0111] (1) The experimental design adopts randomized block de...
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