Biofertilizer for plantation of medicinal materials in Shaolin pharmacy and preparation process thereof
A bio-fertilizer, animal and plant technology, applied in the field of microorganisms, can solve the problems of less obvious effect of microorganisms, unable to kill pathogenic bacteria, difficult to ensure crop safety, etc., and achieve the effect of improving plant quality, improving quality and application effect, and having a significant effect.
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Embodiment 1
[0020] The preparation of embodiment 1 composite microbial decomposing agent
[0021] 1. Preparation of Bacillus megaterium (ACCC10011) fermentation broth: fermentation broth was sterilized at 121° C. for 30 minutes, cooled to 36° C., then inoculated with Bacillus megaterium seed liquid, and the inoculation amount was 0.5% (the inoculum amount was between the seed liquid and the fermentation liquid. proportion). During the fermentation process, the temperature is controlled at 37°C, and the sterile air volume is controlled at 110m 3 / Hour. Ferment for 24 hours. After the fermentation, the number of viable bacteria in the fermented liquid was 1×10 9 -1×10 10 pieces / ml. Both the seed culture medium and the fermentation medium formula are: corn flour 10g, yeast powder 0.5g, bean cake powder 2g, NaCl 0.2g, MgSO 4 0.3g, K 2 HPO 4 1g, CaCO 3 0.5g, 1000ml water, pH7.0-7.2.
[0022] 2. Bacillus licheniformis (AS 1.807) fermentation broth preparation: fermentation broth was s...
Embodiment 2
[0025] The preparation of embodiment 2 composite microbial bacterial agents
[0026]1, the preparation of soybean rhizobia (ACCC15401) fermentation bacterium liquid: fermented culture liquid 121 ℃ of sterilization 30 minutes, cool down to 33 ℃, then inoculate rhizobia seed liquid, inoculum amount is 1.5% (inoculum amount is seed liquid and fermented liquid ratio between). During the fermentation process, the temperature is controlled at 32.5°C, and the sterile air volume is controlled at 75m 3 / Hour. Fermentation end time: 22 hours. After the fermentation, the number of viable bacteria in the fermented liquid was 4×10 10 -6×10 10 pieces / ml. The formula of the fermentation medium is: K 2 HPO 4 0.4g, NaCl 0.2g, MgSO 4 , 0.3g, mannitol, 15g, yeast powder 1.5g, CaCO 3 3g, water 1000mL, pH 6.8-7. The preparation and sterilization process of the seed culture medium is the same as that of the fermentation medium.
[0027] 2, the preparation of fermented bacterium liquid o...
Embodiment 3
[0031] Preparation 1 of embodiment 3 biological fertilizer
[0032] The organic raw material tobacco dust, sugar dregs, bean dregs, bone meal, plant ash, and chili meal are piled up, and the weight ratio is tobacco dust: sugar dregs: bean dregs: bone meal: plant ash: chili dregs=25:15:20:10:15 : 15, and add the compound microbial decomposing agent in embodiment 1 according to the ratio of 0.5%, turn over and throw and mix uniformly by compost turner, add water by 10% weight ratio, then carry out material fermentation. During the fermentation, the color of the material will change from light to dark, and finally dark brown. During this period, it is necessary to measure the temperature of the material with a thermometer, and the temperature of the material is gradually raised from room temperature to 70°C. When the temperature of the material reaches 70°C, keep it for 1-2 days. At this time, turn the pile to increase the oxygen in the material and balance the temperature of th...
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