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Electrophoresis cell with conductive diamond film as electrode

A technology of conductive diamond and diamond film, which is applied in the direction of material analysis, instruments, and measuring devices by electromagnetic means, to achieve the effects of wide potential window, easy cleaning, uniform strength and gradient distribution

Inactive Publication Date: 2011-12-21
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to use the chemical vapor deposition (CVD) diamond film as the electrode of the electrophoresis tank, improve the use effect and life of the electrode, reduce the maintenance cost, and have the advantages of simple preparation method, low cost, no pollution and corrosion resistance Strong, reusable cleaning, strong operability

Method used

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  • Electrophoresis cell with conductive diamond film as electrode
  • Electrophoresis cell with conductive diamond film as electrode
  • Electrophoresis cell with conductive diamond film as electrode

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Embodiment 1

[0022] Embodiment 1: The overall structure of the electrophoresis tank of the present invention

[0023] The overall structure of the electrophoresis tank of the present invention is as follows Figure 1 ~ Figure 3 shown. Figure 1~3 Among them, 1 is the electrophoresis tank; 2 is the conductive diamond film electrode, which is a rectangular electrode with a thickness of about 5 μm to 2000 μm; 3 is the electrode fixing device (for the specific structure of the fixed slot electrode fixing device, refer to figure 2 ); 4 is the wire, connects the power supply and the electrode, and the position where the wire is connected to the electrode is as the criterion so that the wire does not contact the buffered conductive solution; The width of the electrode 2 is adapted so that the conductive diamond film electrode 2 slides down along the two grooves 6 and is stuck in the groove 6; 5 is the lower supporting plate of the electrode fixing device, and when the conductive diamond film el...

Embodiment 2

[0024] Embodiment 2: the making of the conductive diamond film electrode of boron doping

[0025] In the first step, p-type diamond is grown, that is, a boron-doped p-type CVD polycrystalline diamond film is grown. Microwave plasma chemical vapor deposition (MWCVD), hot filament chemical vapor deposition (HFCVD), DC hot cathode CVD or DC jet CVD can be used, using borane or trimethyl borate as boron source; Different substrates (such as silicon, silicon carbide and various industrial electrode materials - molybdenum, niobium, tantalum, titanium, tungsten, zirconium and graphite, etc.) grow boron-doped diamond films.

[0026] The parameters of the growth conditions of the conductive diamond film are listed as one of the methods-microwave plasma CVD method: the substrate is p-type Si, the microwave power is 300-1000W, the pressure is 7-8kPa, the flow rate of hydrogen gas is 200-300sccm, and the flow rate of methane gas is 4 ~6 sccm, boron source uses borane or trimethyl borate,...

Embodiment 3

[0030] Embodiment 3: the making of conductive diamond film electrode doped with nitrogen

[0031] The substrate Si used in the present invention is an n-type (111) single crystal silicon wafer with a thickness of 0.5mm. Using microwave plasma CVD deposition, the experimental gas for preparing conductive diamond film electrodes is H 2 / CH 4 / N 2 , the gas ratio is 110 / 10 / 30sccm. The input power and reaction pressure were 250W and 50Torr, respectively. Deposition time 4h. The resistivity of the thus prepared conductive diamond film is in the range of 10 -3 Ω·cm level.

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Abstract

The electrophoresis tank in which the conductive diamond film is used as an electrode of the invention belongs to the technical field of biological equipment. The structure has an electrophoresis tank (1), electrodes, wires (4) connecting the electrodes and a power supply, and an electrode fixing device (3); the electrode is a conductive diamond film electrode (2), which is formed by chemical vapor deposition. The boron or nitrogen doped diamond film grown on the substrate or the self-supporting boron or nitrogen doped diamond film has a resistivity in the order of 10-4 to 10-2 Ω·cm. The electrode fixing device (3) is a fixed slot type. The electrophoresis nucleic acid and protein molecular results of the conductive diamond film electrode of the present invention are similar to the platinum wire electrode, even better than the platinum wire electrode, and can be easily removed and cleaned repeatedly without damage. After repeated experiments and cleaning electrophoresis The result has no significant change; it has the characteristics of long service life, low maintenance cost, simple preparation method, no pollution, strong corrosion resistance and the like.

Description

technical field [0001] The invention belongs to the technical field of biological instruments. Especially related to electrophoresis tank design, electrode improvement and its preparation method. Background technique [0002] Electrophoresis tank is a common equipment in biochemistry and molecular biology and other related fields. It is mainly used for separation and identification of biological macromolecules, such as nucleic acid and protein. It is widely used in laboratories and biological product factories. Its working structure mainly includes four main parts: electrophoresis tank, electrode, electrolysis buffer and electrode fixing device. Existing electrodes are usually one-dimensional wire electrodes. [0003] General electrophoresis tank electrodes are made of platinum materials. Platinum is a noble metal with good chemical stability and good electrical conductivity. Compared with other metal electrodes, platinum has great advantages, so it becomes the first mate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/453G01N27/447
Inventor 李红东刘钧松樊凤阳吕宪义崔田何志邹广田
Owner JILIN UNIV
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