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Electrochemical enzyme sensor device for detecting blood sugar and preparation method thereof

An enzyme sensor, electrochemical technology, applied in the field of preparation of electrochemical enzyme sensor devices, can solve the problems of glucose diffusion, complicated electrode preparation, etc., and achieve the effects of eliminating interference, high chemical stability, and good electrolytic performance

Inactive Publication Date: 2012-04-25
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, while the selective permeable membrane excludes interfering substances, the diffusion of glucose is also affected; and when detecting actual samples, it is often necessary to use a combination of multiple polymer membranes to completely eliminate interference, making the preparation of the electrode very complicated.

Method used

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  • Electrochemical enzyme sensor device for detecting blood sugar and preparation method thereof
  • Electrochemical enzyme sensor device for detecting blood sugar and preparation method thereof
  • Electrochemical enzyme sensor device for detecting blood sugar and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, the electrolysis efficiency of electrochemical enzyme sensor device

[0030] Accurately weigh 0.23mg of ferrocenecarboxylic acid dissolved in 10mL of 0.1mol L -1 In PBS buffer solution (pH=7.4). The applied potential of the RVC electrode was set to 0.5V, the potential of the gold electrode was set to 0.3V, and 20 μL of ferrocene formic acid solution was injected, and the electrolysis efficiency of the RVC electrode was studied by adjusting the flow rate. Such as figure 2 As shown, when the flow rate is lower than 0.1mL·min -1 When , there is no obvious oxidation current on the gold electrode, that is, there is no electroactive substance in the solution. It shows that under this condition, the electrolysis efficiency of the device is 100%.

Embodiment 2

[0031] Embodiment 2, the mixed solution of glucose and different interferents in FcC 11 Electrochemical response of SH / GOD enzyme electrode

[0032] The following three solutions were injected into the device to measure FcC 11 The SH / GOD enzyme electrode responds to current.

[0033] Solution (1) contains 5.0mmol·L -1 0.1mol·L of glucose -1 PBS buffer solution,

[0034] Solution (2) contains 5.0mmol·L -1 Glucose, 0.1mmol·L -1 0.1mol L of acetaminophen -1 PBS buffer solution,

[0035] Solution (3) contains 5.0mmol·L -1 Glucose, 0.1mmol·L -1 Ascorbic acid, 0.5mmol·L -1 Uric acid and 0.1mmol·L -1 0.1mol L of acetaminophen -1 PBS buffer solution.

[0036] image 3 FcC 11 Response signal of SH / GOD enzyme electrode. Enzyme electrode surface assembled with FcC 11 Mixed double monolayers of SH and GOD. The potential of the RVC electrode is 0.8V, FcC 11 SH / GOD enzyme electrode potential is 0.5V, flow rate is 0.1mL min -1 . 1 for three consecutive injections of gluc...

Embodiment 3

[0037] Example 3, Different Glucose Concentrations and FcC 11 Correlation of SH / GOD Enzyme Electrode Current Response

[0038] First, accurately weigh 0.198g glucose and dissolve it in 10mL PBS solution (containing 0.1mol·L -1 KClO 4 ), the concentration is 100mmol·L -1 , diluted with PBS solution, prepared 0.05, 0.1, 0.3, 0.5, 0.8, 1.0, 3.0, 5.0, 8.0, 10.0, 15.0, 20.0, 30.0 and 40.0mmol·L -1 of glucose solution. Three consecutive injections of different concentrations of glucose solution, FcC 11 The relationship between the current and the glucose concentration of the mixed double monomolecular membrane electrode of SH and GOD is as follows Figure 4 shown. Enzyme electrode at glucose concentration of 0.05-40mmol·L -1 Within the range of FcC 11 The current response of the SH / GOD enzyme electrode is linear with the glucose concentration.

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Abstract

The invention discloses an electrochemical enzyme sensor device for detecting blood sugar and a preparation method thereof. The electrochemical enzyme sensor device consists of four electrode systems, wherein, a reticulated vitreous carbon (RVC) electrode is taken as a first working electrode, a gold electrode is taken as a second working electrode, a glassy carbon electrode is a counter electrode, and silver / silver chloride is taken as a reference electrode. In the electrochemical enzyme sensor device, an RVC material is taken as the first working electrode, and a plurality of electroactive interfering substances in the blood sugar can be electrolyzed by virtue of high electrolysis performance of the RVC material so as to effectively prevent inference of the electroactive interfering substances on the electrochemical glucose sensor. The electrochemical glucose sensor disclosed by the invention has the advantages of good stability, high detection speed, small interference from oxygen and organic and inorganic substances in blood; particularly compared with the existing glucose sensor, the electrochemical glucose sensor disclosed by the invention has the advantages of lower detection limit, low cost and simplicity in operation.

Description

technical field [0001] The invention is an electrochemical enzyme sensor device, in particular to an electrochemical enzyme sensor device for detecting blood sugar that can eliminate the interference of electroactive substances, and also relates to a preparation method of the electrochemical enzyme sensor device for detecting blood sugar. Background technique [0002] Diabetes is a major disease that seriously threatens human health, and its etiology is caused by the lack of insulin or high blood sugar. An effective method for diagnosing and controlling diabetes is to detect blood glucose concentration in real time and reliably. Since Clark and Lyons first proposed the concept of glucose enzyme electrode, people have carried out extensive research on enzyme electrode. The earliest glucose sensor mainly detects the H generated by the enzyme-catalyzed reaction. 2 o 2 , by detecting the released H 2 o 2 The current signal generated by the electrochemical reaction at the an...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30G01N27/327
Inventor 刘又年周飞艨彭勇
Owner CENT SOUTH UNIV
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