Method for preparing biodegradable polymer nano particles

A technology for degrading polymers and nanoparticles, which is applied in the direction of medical preparations and pharmaceutical formulations of non-active ingredients, and can solve the problem of poor controllability of the particle size of biodegradable polymer nanoparticles and unfavorable size of biodegradable polymer nanoparticles. Large-scale preparation, wide size distribution of biodegradable polymer nanoparticles, etc., to achieve the effect of increasing effective action time, good universality, and simple and fast preparation process

Active Publication Date: 2012-06-27
武汉天时维璟微生物科技有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

The work reported by Zhu et al. in International Journal of Pharmaceutic in 2004 and Desai et al. in ACS Nano in 2008 all applied the reprecipitation method to prepare polymeric nanoparticles, but the prepared biodegradable polymer nanoparticles had a wide size distribution and were difficult to use. Accurate quantitative release of drugs
The work that Kawashima et al. reported in European Journal of Pharmaceutics and Biopharmaceutic in 1998 and Trotta et al. in International Journal of Pharmaceutics in 2003 applied the emulsion diffusion method to prepare biodegradable polymer nanoparticles, but the biodegradable polymer prepared by this method The controllability of the particle size of nanoparticles is poor
The work reported by Ma et al. in Industrial & Engineering Chemistry Research in 2009 applied the glass membrane emulsification method to prepare biodegradable polymer nanoparticles. Large-Scale Preparation of Degradable Polymeric Nanoparticles

Method used

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  • Method for preparing biodegradable polymer nano particles
  • Method for preparing biodegradable polymer nano particles
  • Method for preparing biodegradable polymer nano particles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Pass 10 mg / mL of PDLLA (molecular weight: 50,000) chloroform solution and 3.0 mg / mL of PVA aqueous solution through a PVDF membrane with a membrane pore size of 1 μm at a constant speed for 15 times; after that, evaporate the solvent and remove the surfactant by high-speed centrifugation to obtain biological Degradable polymeric nanoparticles, see figure 2 a. The average particle diameter of the biodegradable polymer nanoparticles is 434.3nm, and the polydispersity index PDI is 0.050.

Embodiment 2

[0030] Pass 10 mg / mL PDLLA (molecular weight: 50,000) chloroform solution and 3.0 mg / mL PVA aqueous solution through a PVDF membrane with a membrane pore size of 0.8 μm at a constant speed for 20 times; after that, evaporate the solvent and remove the surfactant by high-speed centrifugation to obtain biological Degradable polymeric nanoparticles, see figure 2 b. The average particle diameter of the biodegradable polymer nanoparticles is 354.5nm, and the PDI is 0.066.

Embodiment 3

[0032] Pass 10 mg / mL PDLLA (molecular weight: 50,000) chloroform solution and 3.0 mg / mL PVA aqueous solution through a PVDF membrane with a membrane pore size of 1 μm at a constant speed for 10 times; after that, evaporate the solvent and remove the surfactant by high-speed centrifugation to obtain bioavailable Degradation of polymeric nanoparticles, see image 3 a. The average particle size of the biodegradable polymer nanoparticles is 520.5nm, and the PDI is 0.066.

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Abstract

The invention provides a method for preparing biodegradable polymer nano particles. The method comprises the following main steps of: mixing an oil phase solution of a biodegradable polymer and an aqueous phase solution of a surfactant; uniformly filtering the mixed solution for a few times through a manual membrane filtering device to obtain biodegradable polymer emulsion with uniform size; and volatilizing the organic solvent to obtain an aqueous solution of the biodegradable polymer nano particles, removing the surfactant by high-speed centrifuging, then dispersing with deionized water, and thus obtaining dispersion of the biodegradable polymer nano particles. By the method, experimental parameters such as the concentration of the biodegradable polymer, the membrane aperture, the membrane filtering times, the concentration of the surfactant, the molecular weight of the biodegradable polymer and the like can be conveniently adjusted, and the medicament release rate can be controlled by effectively controlling the particle diameter; the prepared nano particles have uniform size; control of medicament loading quantity is facilitated, so that the medicament release efficiency is improved, and the effective acting time is increased; and the method is simple and quick, and is suitable for large-scale production.

Description

technical field [0001] The invention belongs to a method for preparing biodegradable polymer nanoparticles, in particular to a method for preparing biodegradable polymer nanoparticles with adjustable size and drug loading. Background technique [0002] Recent studies have shown that polymer nanoparticles have important potential applications in the fields of optics, electricity, magnetism, biotechnology, especially drug delivery and release. Some drugs cannot be used directly due to their strong toxic side effects or the direct use of drugs is not ideal; however, by using drug carriers, many drugs can be circulated in the human body for a long time and released slowly in the lesion, thereby greatly reducing the toxic side effects and Dosing times. Biodegradable polymers are a good choice for drug carriers due to their excellent biocompatibility and biodegradability. [0003] The research results show that the biodegradable polymer nanoparticles with uniform size can contro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/16C08J3/05C08L67/04A61K47/34
Inventor 朱锦涛梁锐晶
Owner 武汉天时维璟微生物科技有限公司
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