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Biological reactor with full-wavelength controllable light sources

A biological reaction device, full-wavelength technology, used in photobioreactors, specific-purpose bioreactors/fermenters, biochemical cleaning devices, etc. Ensure the transmittance and the effect of simple and convenient operation

Inactive Publication Date: 2012-06-27
HUBEI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is currently no scientific instrument that meets the needs of this research

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  • Biological reactor with full-wavelength controllable light sources
  • Biological reactor with full-wavelength controllable light sources

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Experimental program
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Effect test

Embodiment 1

[0025] Such as figure 1 As shown, a bioreactor with a full-wavelength controllable light source includes a peripheral box 4 and a tank 5 made of quartz glass arranged in the peripheral box 4. The top of the tank 5 is provided with a tank cover 14, and Comprising a vent glass pipe 3, a spiral stirrer 2 made of quartz glass arranged in the tank body 5 and one end connected to the tank cover 14, and a temperature and pH value sensor 9 arranged in the tank body 5 and connected to the tank cover 14 at one end , the bottom of the tank body 5 communicates with one end of the fermentation liquid outlet pipe 15 provided with the on-off valve 13, the other end of the fermentation liquid outlet pipe 15 passes through the peripheral box body 4, and the tank cover 14 is provided with a culture medium communicated with the tank body 5 The inlet pipe 8, the end of the culture medium inlet pipe 8 located outside the tank body 5 passes through the peripheral box body 4 and is provided with a s...

Embodiment 2

[0028] A laboratory application method of a biological reaction device with a full-wavelength controllable light source:

[0029] Biological material: Rhodiola rosea isolated cells;

[0030] Culture medium: optimized Rhodiola rosea cell culture medium;

[0031] Research on photoreaction conditions:

[0032] 0-24 hours after inoculation: dark culture, cell adaptation period.

[0033] 24-124 hours after inoculation: 620-630 nanometer light treatment, surface light intensity: 400LUX (lux), which is the light wave condition for rapid cell division and growth. Samples were taken to detect cell growth and salidroside content. 124-168 hours after inoculation: 420-440 nanometers 50% + 620-630 nanometers 50% (50% means that the two light sources each account for half to form a mixed light source and irradiate at the same time, the same applies below), or 320-350 nanometers 40%+ 620-630 nm 60%, or 280-320 nm 10%+620-630 nm 90% light treatment, surface light intensity: 400LUX, which ...

Embodiment 3

[0035] A laboratory application method of a biological reaction device with a full-wavelength controllable light source:

[0036] Biomaterial: Cordyceps militaris ( Cordyceps militaris ) strains;

[0037] Medium: Cordyceps militaris liquid medium;

[0038] Light-controlled fermentation procedure:

[0039] 0-48 hours after inoculation: culture in the dark to allow the strains to adapt and resume growth.

[0040] 48-168 hours after inoculation: 620-630 nanometer light treatment, surface light intensity: 400LUX, for optimal mycelial division growth and adenosine biosynthesis light wave conditions. At this time, the maximum mycelium biomass and maximum adenosine production can be obtained.

[0041] 168-288 hours after inoculation: light treatment at 420-440 nanometers, surface light intensity: 400LUX, optimized light conditions for biosynthesis of cordycepin and carotenoids. At this time, the maximum yield of cordycepin and carotenoids can be obtained.

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Abstract

The invention discloses a biological reactor with full-wavelength controllable light sources. According to the invention, a tank is arranged in a peripheral box; the top of the tank is provided with a tank cover; a helical agitator made of quartz glass is arranged in the tank, and one end of the helical agitator is connected with the tank cover; a temperature and pH value sensor is arranged in the tank, and one end of the temperature and pH value sensor is connected with the tank cover; the bottom of the tank is communicated with one end of a fermentation broth outlet pipe which is provided with a switch valve, and the other end of the fermentation broth outlet pipe penetrates through the peripheral box; the tank cover is provided with a medium inlet pipe communicating with the tank, and one end of the medium inlet pipe located out of the tank is provided with a second sterilizing filter and penetrates through the peripheral box; a sidewall of the tank is provided with a full-wavelength LED device with adjustable wavelength; and an outer wall of the peripheral box is provided with a master controller used for adjusting wavelength of the full-wavelength LED device. The biological reactor has the advantages of selectable full-wavelength light sources, simple operation, accurate control and suitability for biological cell photoreaction research and specific wavelength light-operated cell fermentation production in the fermentation industry.

Description

technical field [0001] The technology of the present invention relates to the field of photoreaction of biological cells, in particular to a bioreaction device with a full-wavelength controllable light source, which is suitable for light control of wild-type living cells or engineering cell genetic research, metabolic research or industrial light-controlled cell fermentation process. Background technique [0002] A large number of scientific studies have proved that the vegetative growth of autotrophic biological cells and the accumulation of secondary metabolites are oriented by the wavelength under the irradiation of a certain wavelength of light source, and the metabolic pathways of heterotrophic biological cells will also change under the stimulation of special wavelength light sources. lead to the accumulation of some biologically active substances. This feature of living cells can be used for industrial production of biologically active substances or drugs needed by hu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/36A01G1/04
CPCC12M31/02C12M3/00C12M41/26C12M41/12C12M1/00C12M21/02C12M37/02C12M27/02C12M41/06C12M31/10
Inventor 董静洲郑小江
Owner HUBEI UNIV FOR NATITIES
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