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Biological dechromizing medium, preparation method thereof and biological dechromizing method

A dechromization and biological technology, applied in chemical instruments and methods, biological water/sewage treatment, water pollutants, etc., can solve the problems of limitation, low concentration of reduced Cr, slow reduction rate, etc.

Inactive Publication Date: 2012-07-04
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the most research on biological dechromation is to use free cells to remove chromium, which is characterized by fast dechromation rate, but has serious disadvantages: free cells are not easy to recycle, and their activity is easy to lose
In addition, most of the currently reported dechromium bacteria have a low tolerance and reduction of Cr(Ⅵ) concentration, and the culture medium and culture conditions required for bacterial growth are also relatively harsh, and most of them are limited to acidic or neutral chromium-containing wastewater. processing, the reduction rate is slow, and there are few industrial applications

Method used

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  • Biological dechromizing medium, preparation method thereof and biological dechromizing method
  • Biological dechromizing medium, preparation method thereof and biological dechromizing method
  • Biological dechromizing medium, preparation method thereof and biological dechromizing method

Examples

Experimental program
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Effect test

Embodiment 1

[0055] Embodiment 1 Preparation of biological dechromium medium and removal of chromium in wastewater

[0056] 1. Preparation of superparamagnetic nanoparticles

[0057] Prepared by co-precipitation method, the reaction principle is: 2Fe 3+ +Fe 2+ +8OH - → Fe 3 o 4 +4H 2 O; press Fe in a 500mL stirred reactor filled with 200mL distilled water 3+ and Fe 2+ Add 8.672mmol ferric chloride (FeCl 3 ·6H 2 O) and 4.336mmol ferrous chloride (FeCl 2 4H 2 0), under the protection of nitrogen, the temperature is raised to 85°C to 90°C, pour 0.375mol concentrated ammonia solution, add 0.26mmol sodium citrate immediately after the reaction system turns black, add 0.26mmol PEI (molecular weight 10172Da ), continue to keep the temperature for 2 hours, and cool to room temperature; after the product is separated by a magnet, it is repeatedly washed with deionized water to obtain a PEI-modified superparamagnetic Fe that is stably dispersed in water. 3 o 4 nanoparticles.

[0058] ...

Embodiment 2

[0068] Example 2 Preparation of Biological Chromization Medium and Removal of Chromium in Wastewater

[0069] 1. Preparation of biological dechromation medium

[0070] Pick the LSSE-09 strain cultured on a nutrient slant and add it to 40 ml of basal medium. The composition of the medium: 1000 ml of deionized water, 10 g / L of tryptone, 5 g / L of yeast extract, and 10 g / L of sodium chloride. After 12-24 hours at 37°C and 150 rpm shaker culture, add it to 500 ml of basal medium according to the inoculum size of 1%, and after 12-36 hours, centrifuge to obtain the bacteria and then use 0.9% physiological Wash with salt water, and repeat three times to obtain the bacteria. Get a certain amount of thalline and place it in the Tris-HCl buffer system (0.05mol / L, pH 9.0), add the magnetic particles prepared in Example 1, so that the ratio between the magnetic particles and the bacteria is 1 / 20 (g / g), mixing thoroughly and uniformly, collecting by applying an external magnetic field, an...

Embodiment 3

[0076] Example 3 Preparation of Biological Chromization Medium and Removal of Chromium in Wastewater

[0077] 1. Preparation of biological dechromation medium

[0078] Pick the LSSE-09 strain cultured on a nutrient slant and add it to 40 ml of basal medium. The composition of the medium: 1000 ml of deionized water, 10 g / L of tryptone, 5 g / L of yeast extract, and 10 g / L of sodium chloride. After 12-24 hours at 37°C and 150 rpm shaker culture, add it to 500 ml of basal medium according to the inoculum size of 1%, and after 12-36 hours, centrifuge to obtain the bacteria and then use 0.9% physiological Wash with salt water, and repeat three times to obtain the bacteria. Get a certain amount of thalline and place it in the Tris-HCl buffer system (0.05mol / L, pH 9.0), add a small amount of magnetic particles prepared in Example 1, so that the ratio between the magnetic particles and the bacteria is 1 / 500 (g / g), fully mixed evenly, collected by an external magnetic field, and the ...

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Abstract

The invention relates to a biological dechromizing method, and provides a biological dechromizing medium. The medium comprises a pannonibacter phragmitetus LSSE-09 strain and polyethyleneimine-modified super-paramagnetic nanoparticles which are adsorbent mutually, wherein the collection number of the strain is CGMCC No.3512. A preparation method of the biological dechromizing medium comprises the following steps of: (1) preparing the olyethyleneimine-modified super-paramagnetic nanoparticles; (2) culturing dechromizing biological bacteria; and (3) preparing and collecting the biological dechromizing medium. The biological dechromizing method disclosed by the invention comprises the following steps of: adding the prepared biological dechromizing medium into waste water containing Cr<6+>; and dechromizing in an aerobic or anaerobic environment. Due to the adoption of the method, mass transfer resistance is greatly lowered, and the industrial prospect is wide.

Description

technical field [0001] The invention relates to the field of biological dechroming, in particular, the invention relates to a biological dechroming medium, a preparation method thereof, and a biological dechroming method. Background technique [0002] Metal chromium has a wide range of industrial uses, and its compounds are widely found in the wastewater discharged from chromium salt production, textile dyeing, dye production, tanning, electroplating and other industries. The common chromium compounds in industrial wastewater are Cr(Ⅲ) and Cr(Ⅵ) in two valence states. Among them, Cr(Ⅲ) is an essential trace element for human body, and its toxicity is one percent of that of Cr(Ⅵ). It is only harmful to plants at higher concentrations, and has low or no toxicity to animals. Cr(Ⅲ) participates in the metabolism of carbohydrates in the body. Adults need to take in 50-200 μg of Cr(Ⅲ) from food every day. If it is lacking, it will lead to hyperlipidemia and high cholesterol (Bara...

Claims

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Application Information

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IPC IPC(8): C02F3/34C02F3/30C02F101/22
Inventor 刘会洲徐林杨良嵘梁向峰
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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