Selenium-rich yeast, selenium-rich yeast hydrolysate and preparation method of the hydrolysate

A technology of selenium-enriched yeast and enzymatic hydrolyzate, which is applied in biochemical equipment and methods, microorganism-based methods, fermentation, etc., can solve problems such as low digestibility, and achieve reduced dosage, high organic selenium content, and high biomass. Effect

Inactive Publication Date: 2012-07-11
广东雅琪生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The yeast itself is wrapped by a thicker cell wall, and the organic selenium that can be really used exists in the yeast cytoplasm. After the yeast selenium product enters the digestive tract, the cell wall of the inactivated yeast cell is not easy to be enzymatically decomposed by the digestive juice, so the digestibility is low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Saccharomyces cerevisiae GABC1003 strain (Saccharomyces cerevisiae GABC1003), also known as selenium-enriched yeast GABC1003 strain (Saccharomyces cerevisiae GABC1003), has been submitted to the Chinese Type Culture Collection Center at Wuhan University for preservation on December 20, 2011, and the preservation number is: CCTCC M 2011474, and identified as alive on 26 December 2011.

[0027] The screening method for the above-mentioned selenium-enriched yeast comprises the following steps:

[0028] 1) According to the physiological and biochemical characteristics of yeast, common yeast strains were cultured in YEPD medium containing 100-200mg / L selenium, and the yeast strain YQ-8 with high selenium content was screened out (selenium content was 2328mg / kg) and yeast strain YQ-118 with high biomass content (biomass is 8.9g / L); YEPD medium: yeast powder 10g, peptone 20g, glucose 20g, distilled water 1000mL, pH 6.0, 115°C moist heat sterilization for 20min.

[0029] 2) Fr...

Embodiment 2

[0033] The preparation method 1 of selenium-enriched yeast enzymatic hydrolyzate of the present invention comprises the following steps: slant strains-liquid strains-first-level liquid seed cultivation-secondary liquid seed cultivation-tertiary liquid seed cultivation-fermentation tank fermentation cultivation-collecting rich Selenium yeast cells-washing-making 10~15% selenium-enriched yeast hydrolyzate-adjusting the pH value to 6.5~7.5-autolysis-adding yeast extract enzyme enzymolysis-separation-concentration-spray drying-selenium-enriched yeast enzymolysis thing. The specific implementation plan is:

[0034] 1) Incline strain: Inoculate selenium-enriched yeast GABC1003 strain (preservation number CCTCC M 2011474) into malt containing 10% of total sugar (starch hydrolyzed sugar) and 100ug / ml of selenium (sodium selenite) The solid slant of the juice was cultured at 28°C and pH 6 for 20 hours, and then stored in a 4°C refrigerator.

[0035] 2) First-level liquid seed culture...

Embodiment 3

[0048] The preparation method 2 of selenium-enriched yeast hydrolyzate of the present invention comprises the following steps:

[0049] 1) Incline strain: Inoculate selenium-enriched yeast GABC1003 strain into wort solid slope containing 20% ​​total sugar (molasses) and 200ug / ml selenium (sodium selenite) at 34°C and pH After culturing for 30 hours under the condition of 5, store them in a refrigerator at 4°C.

[0050] 2) First-level liquid seed culture: Inoculate 5 rings of slant yeast strains into wort medium containing 200ml of 20% total sugar (molasses) and 100ug / ml of selenium (sodium selenite). Stirring and culturing for 30 hours under the conditions of 34°C and pH value of 5 is the liquid strain.

[0051] 3) Second-level liquid seed cultivation: Put the first-level liquid seeds into a 10L Karst jar with an inoculation amount of 3%, which contains 10% of total sugar (molasses) and 200ug of selenium (sodium selenite) / ml of wort culture medium, cultivated for 30 hours a...

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Abstract

The invention discloses a selenium-rich yeast (Saccharomyces cerevisiae) GABC 103 strain which is submitted to be preserved in the China Center for Type Culture Collection, and the serial number is CCTCC M 2011474. The invention also discloses a method for obtaining selenium-rich yeast hydrolysate by utilizing the selenium-rich yeast through fermentation and enzymolysis. The method is simple to operate and low in cost; the organic selenium of per kg of selenium-rich yeast hydrolysate is 1,000-4,000mg and accounts for 92-99% of the total weight of selenium, the content of alpha-amino nitrogen is 2.0-3.0 wt%, so that the selenium-rich yeast and the selenium-rich yeast hydrolysate can be digested and absorbed by animals easily, under the condition of equal volume of addition, the production efficiency is remarkably superior to that of similar products, or the volume of addition can be reduced under the condition of the same production efficiency, and further, the cost is reduced.

Description

technical field [0001] The invention relates to a selenium-enriched yeast, a method for producing a selenium-enriched yeast hydrolyzate by using the yeast, and the obtained selenium-enriched yeast hydrolyzate. Background technique [0002] Selenium is the active center of glutathione peroxidase (GPx). The enzymatic reaction of GPx can remove free radicals and lipid peroxides in animals, and prevent excessive free radicals and peroxides in animals from damaging cell membranes, resulting in Cell dysfunction and necrosis. Selenium can be used as a stimulator of the animal immune system, which can promote the production of antibodies, strengthen the immune system and anti-infection ability of the animal body. It can also improve the reproductive performance of animals. Selenium deficiency can lead to a decrease in the number and vitality of male animals' sperm and an increase in the rate of sperm deformity. [0003] Selenium is highly toxic to animals: long-term intake of 5-10...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/18C12P1/02C12R1/865
Inventor 陈训银董爱华徐春洪胡怀文吴迪冯业梁红
Owner 广东雅琪生物科技股份有限公司
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