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Application of functionalized nano-selenium in tumor angiogenesis inhibiting and antitumor drugs

A tumor angiogenesis and anti-tumor drug technology, applied in the direction of anti-tumor drugs, drug combinations, sulfur/selenium/tellurium active ingredients, etc. Effects directly saved and used

Inactive Publication Date: 2012-08-15
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] So far, no reports have been found about the use of nano-selenium in the preparation of drugs for inhibiting tumors and tumor angiogenesis

Method used

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  • Application of functionalized nano-selenium in tumor angiogenesis inhibiting and antitumor drugs
  • Application of functionalized nano-selenium in tumor angiogenesis inhibiting and antitumor drugs
  • Application of functionalized nano-selenium in tumor angiogenesis inhibiting and antitumor drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The preparation of the nano selenium of embodiment 1 heteropolyacid-transferrin modification

[0043] (1) Weigh 1.5g of dodecatungstophosphoric acid and dissolve it in 2mL of distilled water to prepare a 0.5mol / L storage solution, adjust the pH to 3.5~5.5 with 1mol / L HAc-NaAc buffer solution, and set the temperature in N 2 Under the atmospheric atmosphere, use a potentiostat to control potential electrolytic reduction, and the degree of reduction is indicated by a copper coulomb meter. When the electrolysis reaches two-electron reduction, the electrolysis is stopped, and the electrolyte is placed in a vacuum desiccator to precipitate crystals. The crystals were recrystallized 2 to 3 times to obtain the treated heteropolycyanate compound.

[0044] (2) Prepare dodecatungstophosphate heteropoly blue into a 0.5mmol / L solution, add it dropwise to 0.5-1mmol / L sodium selenite solution and mix evenly under continuous stirring, and finally dodecatungsten The molar ratio of hete...

Embodiment 2

[0046] The preparation of the nano selenium of embodiment 2 gallic acid-transferrin modification

[0047] Na 2 SeO 3 Prepare a 5mmol / L solution with distilled water, prepare a 5mmol / L solution of gallic acid with distilled water, add the gallic acid solution dropwise to the sodium selenite solution under constant stirring, and adjust the pH with 0.1mol / L hydrochloric acid solution 2.8-3.5, heated in a 70°C water bath until the solvent volatilized and dried to obtain an orange-red nano-selenium sample.

[0048] (2) get above-mentioned nano selenium and be mixed with 0.5mmol / mL solution, adjust its pH between 2.3~5.0 with the hydrochloric acid solution of 0.1mmol / mL, transferrin is mixed with the solution of 0.2mmol / mL with triethanolamine, in Add it dropwise to the above-mentioned nano-selenium solution under constant stirring, keep the pH of the solution between 2.3 and 5.0 during the process, continue to stir for 12 to 24 hours, place it at 4°C for 12 hours, centrifuge, was...

Embodiment 3

[0049] Embodiment 3 as the in vitro experiment of antitumor drug

[0050] In this experiment, a variety of tumor cell lines purchased from ATCC were selected, including human malignant melanoma cells (A375), human liver cancer cells (HepG2), human colon cancer cells (SW480), human prostate cancer (PC-3), and human breast cancer cells. cells (MCF-7), human lung adenocarcinoma cells (A549). The sample numbers tested are: a (nano-selenium modified by dodecatungstophosphoric acid-transferrin), b (nano-selenium sol modified by dodecatungstophosphoric acid), c (nano-selenium modified by gallic acid-transferrin), d (gallic acid modified nano-selenium sol) and cisplatin.

[0051] (1) MTT test method

[0052] Take tumor cells in the logarithmic growth phase and adjust the concentration of viable cells to 2×10 4 / ml was added to a 96-well culture plate, 100 μl per well, cultured in an incubator for 24 hours, and then added 100 μl of test samples with different concentrations. The neg...

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Abstract

The invention discloses an application of functionalized nano-selenium in tumor angiogenesis inhibiting and antitumor drugs. The inventor finds that the functionalized nano-selenium not only has an antitumor effect, but also has a tumor angiogenesis inhibiting effect, so that the functionalized nano-selenium can be used as an active component to be applied in tumor angiogenesis inhibiting and antitumor drugs. The functionalized nano-selenium prepared in the invention, such as heteropoly acid-transferrin modified nano-selenium or gallic acid-transferrin modified nano-selenium, is prepared by directly coupling excessive heteropoly acid or gallic acid with elemental nano-selenium, the preparation process dispenses with addition of other auxiliary reagents, the product system is simple, and the product can be directly preserved and used. The modifier adopted in the invention improves the targeting property and the transport and transmembrane absorption in cells of the functionalized nano-selenium, and can increase the drug intake of cells and reduce drug efflux, thereby guaranteeing that the drug in the cells is maintained at a higher level.

Description

technical field [0001] The invention relates to the application of functionalized nano-selenium, in particular to the application of functionalized nano-selenium in inhibiting tumor angiogenesis and antitumor drugs. Background technique [0002] Cancer has become the number one killer threatening human life and health. Countries all over the world attach great importance to the prevention and treatment of cancer, and the development of highly efficient, low toxicity, broad-spectrum, and highly selective anticancer drugs has become a long-term strategic goal. [0003] Tumor growth, deterioration, invasion and metastasis are closely related to neovascularization, and there is a mutual promotion relationship between neovascularization and tumor growth. On the one hand, tumor cells induce new capillaries to grow from the host vessel wall in the surrounding tissue by secreting certain specific pro-angiogenic factors, grow toward the tumor, and further penetrate into the interior ...

Claims

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Application Information

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IPC IPC(8): A61K47/48A61K33/04A61P35/00
Inventor 刘杰周艳晖刘亚楠孙冬冬张蓉
Owner JINAN UNIVERSITY
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