Antihypertensive active rapeseed peptide and preparation method and application thereof

A technology for lowering blood pressure and active peptide is applied in the field of preparation of rapeseed active peptide for lowering blood pressure. The effect of small intestinal reaction and strong protein hydrolysis ability

Active Publication Date: 2012-08-22
NANJING UNIV OF FINANCE & ECONOMICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are about 200 million hypertensive patients in my country, and there are still 10 million new people every year. The existing blood pressure lowering drugs have the following problems: On the one hand, long-term use of the existing blood pressure lowering drugs will cause many side effects to the body, especially It has great damage to the digestiv...

Method used

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  • Antihypertensive active rapeseed peptide and preparation method and application thereof
  • Antihypertensive active rapeseed peptide and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Simulated gastrointestinal tract digestion and hydrolysis of rapeseed meal to prepare crude extract of antihypertensive rapeseed active peptide

[0025] method one)

[0026] (1) Crush the rapeseed meal, pass through a 100-mesh sieve, use the crushed rapeseed meal as the raw material, and mix the raw material with water at a solid-to-liquid ratio of 1:20, adjust the pH value to 2.0, and the temperature is 36.5°C to 37.5°C °C, stir and disperse for 1 h to obtain rapeseed protein liquid.

[0027] The source of the rapeseed meal is common rapeseed or double-low rapeseed, and the particle size of the crushed rapeseed meal is 10-200 μm.

[0028] It is also possible to use protein isolated from rapeseed meal instead of pulverized rapeseed meal to prepare rapeseed protein liquid.

[0029] (2) According to the mass ratio of the pepsin added to the protein in the rapeseed protein liquid of 1 to 10:100, add pepsin to the rapeseed protein liquid, control the temperature...

Embodiment 2

[0035] Example 2 Preparation of Rapeseed Active Peptide for Blood Pressure by Refining the Crude Extract of Rapeseed Active Peptide for Blood Pressure

[0036] The blood pressure lowering rapeseed active peptide is refined to obtain the blood pressure lowering rapeseed active peptide.

[0037] Refining method 1: The crude extract of the antihypertensive rapeseed active peptide is ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 3kDa, the permeate (all polypeptides with a molecular weight less than 3KDa) is desalted by dialysis, and freeze-dried to obtain the antihypertensive rapeseed active peptide , the antihypertensive rapeseed active peptide has both renin inhibitory activity and ACE inhibitory activity.

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Abstract

The invention provides an antihypertensive active rapeseed peptide and a preparation method and application thereof. The preparation method of the antihypertensive active rapeseed peptide includes obtaining crude extract of the antihypertensive active rapeseed peptide by pepsin and pancreatic enzymatic hydrolyzed rapeseed protein, and obtaining the antihypertensive active rapeseed peptide by refining. The invention further provides the antihypertensive active rapeseed peptide prepared by the method and application thereof. Sources of rapeseed meal for preparing the antihypertensive active rapeseed peptide are extensive, production process is simple, low in cost and environment-friendly, and the method is suitable for industrialization. The antihypertensive active rapeseed peptide causes low gastrointestinal reaction, is capable of resisting hydrolysis of human digestive enzymes, and has good antihypertensive effect by actively reacting to target organs. The antihypertensive active rapeseed peptide is capable of inhibiting not only ACE (angiotensin converting enzyme) but also renin activity, is capable of avoiding alternative pathways of a RAS (renin angiotensin system) activated by taking antihypertensive drug, ACE inhibitor, for long, and has bright application prospect in terms of lowering blood pressure.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the antihypertensive rapeseed active peptide, its preparation method and application. Background technique [0002] my country is the country with the largest rapeseed production in the world, with an annual output of nearly 12 million tons. Rapeseed meal is the main by-product of rapeseed oil processing, accounting for 65% of the total rapeseed, which contains 35% to 42% protein. The amino acid composition of rapeseed protein is reasonable, and the content of lysine, cystine and methionine is relatively high, so it is an excellent vegetable protein. The development and utilization of rapeseed protein resources has great social significance and good economic benefits for the effective use of our country's plant protein resources and the improvement of the nutritional level of our people. [0003] At present, there are about 200 million hypertensive patients in my country, and there ...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34C07K1/22C07K1/14C07K2/00A61K38/01A61P9/12
Inventor 袁建王立峰鞠兴荣何荣高瑀珑陈银基都立辉石嘉怿周建新
Owner NANJING UNIV OF FINANCE & ECONOMICS
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