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Chickweed polysaccharide composition and applications thereof in preparation of antiviral medicine

An antiviral drug, chickweed polysaccharide technology, applied in the field of medicine, can solve problems such as no reports of mixed application, no related reports of homogeneous chickweed polysaccharides, etc., achieves broad-spectrum antiviral activity, high development value, Simple effect of clinical medication

Inactive Publication Date: 2012-10-03
贾晓斌
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation method of chickweed polysaccharide and its composition described in Chinese Patent Publication No. CN1521193A points out that chickweed polysaccharide composition is a mixture of 80-90% total flavonoids and 10-20% crude polysaccharide, and has certain anti-AIDS The role of viruses and herpes viruses is only the extraction and preparation of chickweed polysaccharides. The obtained polysaccharides contain proteins, pigments and other components. Ratio-mixed chickweed polysaccharide composition and its application in the preparation of antiviral drugs

Method used

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  • Chickweed polysaccharide composition and applications thereof in preparation of antiviral medicine
  • Chickweed polysaccharide composition and applications thereof in preparation of antiviral medicine
  • Chickweed polysaccharide composition and applications thereof in preparation of antiviral medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Anti-Hepatitis B Virus Research of Chickweed Polysaccharide Composition in Vitro

[0029] In this experiment, HepG2.2.15 cell model, a classic in vitro anti-HBV drug screening model, was used. Different concentrations of chickweed polysaccharide compositions were administered to HepG2.2.15 cells for several days. ), e antigen (HBeAg) and HBV DNA secretion to judge whether the drug is effective, and the MTT method was used to measure the cytotoxicity of different chickweed polysaccharide compositions during the experiment. Based on the above experimental design, the anti-hepatitis B virus pharmacodynamics study was carried out on the chickweed polysaccharide composition in vitro, and lamivudine (3TC), which was confirmed in clinical application, was used as a positive control drug to evaluate the efficacy of the chickweed composition. Anti-HBV effect.

[0030] Cytotoxicity When the HepG2.2.15 cells proliferated to a certain extent and the antigen secretion was...

Embodiment 2

[0045] Example 2 Anti-herpes virus research of chickweed polysaccharide composition in vitro

[0046]Five kinds of chickweed polysaccharides were mixed evenly at the content ratio of 1%, 40%, 20%, 17%, and 22%, respectively, to obtain the chickweed polysaccharide composition, and the cell model HepG2 cell model in Example 1 was used to inoculate herpes in different ways Virus HSV-1HS-1 and HSV-2333 strains were used to investigate their anti-herpes virus activity in vitro. Using Vero cells as a model, equal volumes of different concentrations of chickweed polysaccharide compositions (1, 3, 10, 30, 60, 100, 200 μg / mL) and virus dilution (100 TCID) were mixed and added directly to the single layer Vero cells, cultured in a 37°C, 5% CO2 cell incubator for 48 hours, and observed the cytopathic effect (CPE) under a light microscope. From the analysis of the experimental data (the table shows that there is no significant difference between the Vero cells in the experimental group w...

Embodiment 3

[0054] Example 3 Anti-Coxsackievirus Research of Chickweed Polysaccharide Composition in Vitro

[0055] Five kinds of chickweed polysaccharides were mixed uniformly at the content ratio of 43%, 5%, 11%, 11%, and 30% respectively, and the direct inactivation effect of the chickweed polysaccharide composition on Coxsackie virus (CVB3) was investigated. On the plate, add 1 mL / well of cardiomyocytes at a concentration of 0.5×104 / mL, and incubate for 24 hours. Except for the normal group, add 1 mL of CVB3 at 100 TCID per well. After incubation for 1 hour, discard the supernatant, wash with PBS for 3 times, and add different concentrations of Chickweed polysaccharide composition (3, 10, 30, 60, 100 μg / mL), ribavirin 1mmol / mL was added to the positive control group, 4 wells in each group, cell maintenance solution was added, cultured in a 5% CO2 incubator for 72 hours, and the cells were observed daily Lesion effect (CPE), and the cell survival rate was detected by MTT assay.

[005...

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Abstract

The invention relates to a chickweed polysaccharide composition and applications thereof in preparation of an antiviral medicine. The chickweed polysaccharide composition disclosed by the invention is prepared by mixing five kinds of chickweed polysaccharides (A, B, C, D and E) according to a certain proportion and is characterized in that the composition is of brown floccule which is easily dissolved in water and is not dissolved in ethanol, acetone, diethyl ether, n-butanol and isopropanol with high concentration; the polysaccharide composition contains five kinds of chickweed polysaccharides with molecular weights ranging from 5000 to 30000 and is formed by rhamnose, arabinose, xylose, mannose, glucose and galactose according to certain molar ratio. After being mixed uniformly in a special proportion, the five kinds of the polysaccharides can be used for strengthening antiviral activities by utilizing mutual synergy. The invention further discloses a use of the chickweed polysaccharide composition in preparation of the antiviral medicine, which is used for treating the diseases such as hepatitis B virus, HIV (human immunodeficiency virus), herpes virus, influenza virus, enterovirus and adenovirus and the like and can be prepared into various pharmaceutic preparations. The composition has a good therapeutic effect, no toxicity and wide prospect in development of new antiviral medicines.

Description

technical field [0001] The present invention relates to the application of the chickweed polysaccharide composition in the preparation of antiviral drugs, in particular to a chickweed polysaccharide composition which is separated and purified according to the active ingredients of traditional Chinese medicine and then mixed uniformly in a certain proportion and its application in the preparation of antiviral drugs ; The present invention also relates to preparations and health products containing chickweed polysaccharide composition, belonging to the field of medicine. Background technique [0002] Virus is the smallest pathogenic microorganism. It is a kind of microorganism with no cell structure and life characteristics such as inheritance and replication. Its core is ribonucleic acid or deoxyribonucleic acid (DNA). One of the most infectious diseases. Common diseases caused by viruses include infectious hepatitis, herpes, encephalitis, viral respiratory tract infections,...

Claims

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Application Information

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IPC IPC(8): A61K31/715A61P31/12A61P31/20A61P31/18A61P31/22A61P31/16A61P31/14C08B37/00
Inventor 贾晓斌马利华
Owner 贾晓斌
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