Method for preparing protein ribonuclease modified copper sulfide nanometer material

A protein ribonucleic acid and ribonuclease technology, which is applied in the field of functional materials and drug treatment materials, can solve problems such as harsh reaction conditions and complex reaction steps, and achieve the effects of good biocompatibility, simple operation and mild conditions.

Inactive Publication Date: 2012-10-03
SHANGHAI JIAO TONG UNIV
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  • Abstract
  • Description
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Problems solved by technology

However, these methods generally require complex reaction steps and harsh re

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Using water as a solvent, mix 5 ml of 50 mmol / L copper nitrate and 5 ml of 12.5 mg / L ribonuclease, adjust the pH to 5.5, and stir for 3 minutes at 25°C. Then add 5 milliliters of 10 mmol / L sodium sulfide, stir and react at 25 degrees Celsius for 5 minutes, then move the solution to a thermostat to keep at room temperature and react for 100 minutes to obtain copper sulfide nanospheres with a diameter of 100-200 nm.

Embodiment 2

[0022] Using water as a solvent, mix 5 ml of 100 mmol / L copper nitrate and 5 ml of 12.5 mg / L ribonuclease, adjust the pH to 8, and stir at 25°C for 30 minutes. Then add 5 milliliters of 100 mmol / L sulfurized acetamide, stir and react at 25 degrees Celsius for 5 minutes, then move the solution to a constant temperature box and keep it at 60 degrees Celsius for 30 minutes to obtain copper sulfide nanospheres with a diameter of 100-200 nm.

Embodiment 3

[0024] Using water as a solvent, mix 5 ml of 200 mmol / L copper chlorate and 5 ml of 50 mg / L ribonuclease, adjust the pH to 12, and stir for 60 minutes at 25°C. Then add 5 milliliters of a mixture of 50 mmol / L thiourea and ammonium thioacetate, stir and react at 25 degrees Celsius for 5 minutes, then move the solution to an incubator and keep it at 90 degrees Celsius for 20 minutes to obtain a sulfide with a diameter of 100-300 nm. copper nanospheres.

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Abstract

The invention discloses a method for preparing protein ribonuclease modified copper sulfide nanometer material. The method includes: firstly, preparing chelated solution, and crosslinking amino acid groups of protein crystals of protein ribonuclease with semiconductor metal ions; secondly, adding a sulfur source, using protein crystal clusters as a framework, combining excessive copper and sulfur ions in a reaction system with driving copper sulfide nano particles to growth continuously, and controlling reaction time and reactant concentration to obtain a spherical nano structure. The copper sulfide spherical nano structure significantly promotes specialties of multiple disciplinaries such as photovoltaic conversion, photocatalysis, drug controlled-release and gene carriers. The method is simple in operation, mild in condition, low in cost and environment-friendly, the synthesized product copper sulfide nano balls have water solubility and stability and high biocompatibility, and the method is a simple method for preparing functional nano materials.

Description

technical field [0001] The invention relates to a method for preparing protein ribonuclease protein crystal-modified copper sulfide nanospheres, which belongs to functional materials and medical treatment materials. Background technique [0002] Semiconductor sulfide materials have been widely used in optical conductors, medical imaging, and solar cells due to their unique optical, electrical, and thermal properties. Although the preparation, size and shape control of semiconductor sulfides have always been the research difficulties, due to the incomparable nanoscale effects of nanomaterials compared with other material effects, such as larger specific surface area and larger stock displacement. The latest research shows that semiconductor quantum dots such as copper sulfide, silver sulfide and lead sulfide have good optical properties in the near-infrared band, and are an ideal material for biomedical imaging, as well as nano-semiconductor sulfide and titanium oxide. Nanos...

Claims

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Application Information

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IPC IPC(8): B82B3/00A61K47/42A61K47/02A61K48/00B82Y40/00
Inventor 崔大祥陈俊阮静孔毅飞
Owner SHANGHAI JIAO TONG UNIV
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