Method for preparing hedysarum polybotrys extract by adopting coupling technology

Abstract

The invention provides a method for preparing a hedysarum polybotrys extract by adopting a coupling technology. The method comprises the following steps of: carrying out heating reflux extracting on hedysarum polybotrys; adding commercially available pectinase in a water extracting solution with the temperature of 15-60 DEG C; carrying out enzymolysis and sedimentation; removing insoluble impurities to obtain an enzymolysis extracting solution; carrying out ultra-filtering on the enzymolysis extracting solution; or, directly carrying out ultra-filtering on the water extracting solution; carrying out ultra-filtering on diluted trapped fluid for 2-4 times under the same condition, i.e. diluting the trapped fluid subjected to ultra-filtering each time and then carrying out ultra-filtering on the diluted trapped fluid subjected to ultra-filtering, ensuring that the volume of the trapped fluid subjected to ultra-filtering for the last time does not exceed 20 percent of the volume of the enzymolysis extracting solution, and merging all filtrated solutions to obtain a total filtrated solution; carrying out nanofiltering and concentrating on the total filtrated solution; concentrating trapped fluid subjected to nanofiltering under reduced pressure, and drying to obtain a hedysarum polybotrys full-ingredient extract; or concentrating the trapped fluid subjected to nanofiltering to be an extracting solution with corresponding concentration for preparing liquid preparation. According to the method, the process period for preparing the hedysarum polybotrys extract can be effectively shortened, the production cost is reduced, the extract quality is improved, and environmental protection is favored.

Description

technical field [0001] The invention belongs to the technical field of extracting and purifying herbal medicines, and relates to a method for preparing an extract of Radix Radix, in particular to a method for purifying and concentrating an extract of Radix Radix by enzymolysis-ultrafiltration-nanofiltration. Background technique [0002] As one of the authentic medicinal materials in Gansu Province, Heqi has a long history of application and is well-known at home and abroad. Prunus astragalus, a plant of the leguminous genus Prunus Astragalus ( Hedysarum polybotrys The dried root of Hard.-Mass) is mainly used in traditional Chinese medicine to invigorate qi and solidify the surface, diuretic to relieve toxins, drain pus, astringe sores and promote granulation. Modern pharmacological studies have shown that Radix Astragali has the effects of regulating the body's immunity, strengthening the heart, lowering blood pressure, protecting the liver, lowering blood sugar, and anti-...

AI Technical Summary

Problems solved by technology

However, due to the wide solubility range of water, impurities are easily dissolved during extraction, resulting in many impurities in the extract, low relative content of active substances, easy to absorb moisture, and not conducive to the preparation of subsequent preparations

2) Red Qi plus water reflux extraction-moderate concentration-ethanol precipitation-recovery of ethanol-decompression concentration-drying process, this process adds ethanol precipitation purification process to remove macromolecular impurities, resulting in a high loss rate of active ingredients, especially in Radix Radix Macromolecular hydrophilic polysaccharides are basically lost; and the extract needs to be concentrated before precipitation, and ethanol needs to be recovered after precipitation and purification, resulting in long process flow and high production costs

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