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Centrifugal micro-fluidic chip for detecting nitrate and nitrite and preparation method of centrifugal micro-fluidic chip

A microfluidic chip, nitrite technology, applied in chemical instruments and methods, measuring devices, laboratory containers, etc., can solve problems such as no substantial breakthroughs, and achieve good application prospects and parallel detection capabilities. High and shortened detection time effect

Inactive Publication Date: 2012-11-21
SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, a wafer-shaped chip is used to prepare microstructures and microchannels on its surface for analysis and detection, without the need for external driving pumps and valves, relying on centrifugal force to drive and control sample microfluidics, and complete the centrifugal microfluidics of several sample analysis and detection at the same time At present, there has been no substantial breakthrough in the application field of rapid detection of nitrate and nitrite.

Method used

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  • Centrifugal micro-fluidic chip for detecting nitrate and nitrite and preparation method of centrifugal micro-fluidic chip

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Effect test

Embodiment 1

[0029] The microstructure and microchannel graphics of the centrifugal microfluidic chip for detecting nitrate and nitrite were designed and drawn by computer-aided design software. The microstructure and microchannels of the three-layer cycloolefin copolymer (COC) disc chip were prepared by CNC machining, and the chips of each layer were cleaned with tap water and distilled water respectively, and the remaining fingerprints, oil stains and other stains on the surface of the chip were wiped with ethanol. On the two layers of double-sided adhesive film, the required microstructure and microchannel are prepared by cutting plotter. Weigh 5mg 3. After being directly placed in the derivatization reaction sample cell (detection cell), the three-layer COC chip and the two-layer double-sided adhesive film are carefully aligned, bonded, pressure-sealed, and the detection chip is made. Add 100 μL of standard solution or water sample to be tested at the sample injection hole, and mix t...

Embodiment 2

[0031] Design and draw the microstructure and microchannel graphics of the centrifugal microfluidic chip with computer-aided design software. The microstructure and microchannel of the three-layer polymethyl methacrylate (PMMA) disc chip were prepared by CNC machining, and the chips of each layer were cleaned with tap water and distilled water, and the residual fingerprints and oil stains on the chip surface were wiped with ethanol. On the two layers of mechanoadhesive film, the required microstructure and microchannel were prepared by cutting machine. Weigh 8mg 6 and 5mg 3 respectively placed in the reduction reaction sample cell and the derivatization reaction sample cell. The three-layer PMMA chip and the two-layer mechanoadhesive film were carefully aligned, bonded, and pressure-sealed to make a centrifugal microfluidic chip for detecting nitrate and nitrite. Add 100 μL of standard solution or water sample to be tested at the sample injection hole, and the centrifugal...

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Abstract

The invention relates to a centrifugal micro-fluidic chip for detecting nitrate and nitrite and a preparation method of the centrifugal micro-fluidic chip. The centrifugal micro-fluidic chip is a disk type centrifugal micro-fluidic chip with a microstructure and a microchannel; the mixing, reacting and separating process of a sample and a reagent is finished under the drive of a centrifugal force generated by rotating a centrifugal machine; and the contents of nitrate and nitrite in the sample are quantitatively or qualitatively detected by an ultraviolet visible light spectrophotometer. According to the centrifugal micro-fluidic chip, the required reagents and samples are fewer; the pretreatment is avoided; and a plurality of samples can be simultaneously treated and detected, and thus the integration, the micromation and the automation of the detection are realized; and the centrifugal micro-fluidic chip has the characteristics of economy, quickness, portability and high efficiency and provides a brand-new analysis technology for quickly and quantitatively detecting the nitrate and the nitrite in water bodies, soil and foods.

Description

technical field [0001] The invention relates to a centrifugal microfluidic chip for detecting nitrate and nitrite. The centrifugal microfluidic chip is a disc-shaped chip with microstructures and microchannels on the surface, and is driven by the centrifugal force generated by the rotation of the centrifuge to realize During the mixing, reaction and separation process of samples and reagents, the content of nitrate and nitrite in samples on the chip can be quantitatively or qualitatively detected by UV-visible spectrophotometer, which is mainly used in health quarantine, food safety, environmental protection, water quality monitoring, etc. Related field. Background technique [0002] With the rapid development of social economy and the over-exploitation of environmental resources, the resulting environmental pollution has aroused increasing public concern. For example, the nitrate and nitrite pollution caused by nitrogen-containing pesticides, chemical nitrogen fertilizers ...

Claims

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Application Information

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IPC IPC(8): B01L3/00G01N21/01G01N21/33
Inventor 沙俊王晓东聂富强
Owner SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
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