Method for identifying proanthocyanidins in ginkgo leaf preparation
A technology of proanthocyanidins and ginkgo biloba, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of endangering the drug safety of the people, fake ginkgo biloba preparations, and low detection standards, so as to achieve easy identification of authenticity and simple operation steps Less, quicker analysis and identification of effects
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Embodiment 1
[0040] Example 1 Detection of ginkgo leaf proanthocyanidins and comparison with grape seed proanthocyanidins
[0041] Take homemade Ginkgo biloba proanthocyanidin extract (95% proanthocyanidin content) and grape seed proanthocyanidin extract (96% proanthocyanidin content), respectively dissolve in methanol, filter, and perform HPLC detection:
[0042] Equipment: Aglient 1200 high performance liquid chromatograph; Diamonsil C18 chromatographic column 5μm 4.6×150mm
[0043] Chromatographic conditions:
[0044] (1) The stationary phase is a phenomenex diol column (250×4.6 mm, 5 μm);
[0045] (2) The mobile phase is (A) acetonitrile, (B) methanol / water / acetic acid (95 / 3 / 2, V / V / V); the gradient of the mobile phase is 0~10 min: 0~12 %B; 10~ 10.01 min: 12-15 %B; 10.01-30 min: 15-45 %B; 30-35 min: 45 %B; 35-40 min: 45-0 %B;
[0046] (3) The flow rate is 1.0 ml / min;
[0047] (4) Detection wavelength: fluorescence excitation wavelength 276 nm; emission wavelength 316 nm;
[0048] (...
Embodiment 2
[0050] Example 2 Analysis of proanthocyanidins in Ginkgo biloba leaves
[0051] Sample source: Ginkgo biloba leaves (manufacturer Zhejiang Kangenbei Pharmaceutical Co., Ltd., batch number 20100709)
[0052] Sample 1: Take 2 tablets, grind to fine powder, dissolve with methanol, filter, and obtain.
[0053] Sample 2: Take 2 tablets, grind them to fine powder, dissolve them with 16ml 20% acetone solution and ultrasonic assistance, filter to remove insoluble excipients; apply the filtrate to 30-60 mesh polyamide resin, and use 3 4 times the volume of 20% acetone solution to elute, discard the part; then use 3 to 4 times the volume of 80% acetone solution to elute to obtain the 80% acetone part; recover the 80% acetone part to dryness; the residue is washed with methanol Dissolved to obtain a methanol solution.
[0054] Sample 3: Take 2 tablets, grind them to fine powder, dissolve them with 16ml 20% acetone solution and ultrasonic assistance, filter to remove insoluble excipient...
Embodiment 3
[0057] Example 3 Analysis of Proanthocyanidins in Ginkgo Leaf Capsules
[0058] Sample source: Tianbaoning Capsules (health care products, produced by Hangzhou Kangenbei Pharmaceutical Co., Ltd., batch number 20110516)
[0059] Take 2 capsules, open the capsules, pour out the contents, grind to a fine powder, dissolve with 16ml 20% acetone solution, ultrasonic assistance, filter, and remove insoluble excipients. Load the filtrate on a 30-60 mesh polyamide resin, elute with 3-4 times the volume of 20% acetone solution after loading, discard the part; then elute with 3-4 times the volume of 80% acetone solution , to obtain 80% acetone part; recover 80% acetone part until there is no acetone smell, extract twice with an equal volume of butyl acetate, discard the organic phase, recover the water phase to dryness; dissolve the residue with methanol to obtain a methanol solution.
[0060] Carry out the separation of proanthocyanidins with the chromatographic condition among the emb...
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