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Microorganism used for decolouring molasses alcohol waste water and decolouring method

A technology for molasses alcohol wastewater and microorganisms, which is applied in the field of screening and separating the above microorganisms, biological decolorization of molasses alcohol wastewater, and microorganisms for decolorization of molasses alcohol wastewater. There are many problems such as large differences in the rate, so as to achieve the effect of excellent strains and decolorization methods, good biological decolorization efficiency, and reduction of secondary pollution

Active Publication Date: 2012-12-26
GUANGXI UNIV
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  • Abstract
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Problems solved by technology

[0006] On the other hand, different wastewater types and sources, different production seasons, and different wastewater pretreatment methods may affect the decolorization degradation efficiency and color value determination
This is also one of the reasons why it is difficult to compare the reported values ​​in the literature on the decolorization rate of molasses alcohol wastewater
For example, Kumar et al found that the 10-day decolorization rates of versicolor versicolor and Phanerochaete chrysosporium to the concentration of 6.25% of the molasses alcohol wastewater treated by the anaerobic reactor were 71.5% and 53.5%, respectively. The decolorization rate of the molasses alcohol wastewater with a concentration of 25.0% was 19.5% and 16.5% respectively; the thermophile Aspergillus fumigatus G-2-6 reported by Ohmomo et al. found that the dialysis treatment of molasses alcohol was effective in the continuous decolorization process of molasses wastewater. The decolorization rate of wastewater reaches 70%, but the decolorization rate of wastewater without dialysis treatment is only 40%; the decolorization rate of acetogenic bacteria No.BP103 reported by Sirianuntapiboon et al. The difference is very large, respectively 32.3 ± 3.2% and 73.5 ± 3.5%. Similarly, the decolorization rate of Issa orientalis to the molasses wastewater after anaerobic treatment reached 90% in 7 days, but the decolorization rate to the untreated molasses wastewater was only 50%. -60%

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Pick a little preserved Aspergillus flavus (Aspergillus flavus) strain and inoculate it into the PDA test tube slant medium, the medium is glucose 15g / L, NaNO 3 3.0g / L,KH 2 PO 4 1.0g / L, MgSO 4 ·7H 2 O 0.1g / L, agar 15g / L, pH 4.5-9.0, rotation speed 100-400rpm, cultured in a biochemical incubator at 28°C for 5 days. Wash the spores with 0.5% Tween80 solution, pour into a shaker flask equipped with glass beads, oscillate to disperse the spores, filter to obtain a spore suspension, and measure the concentration of the spore suspension with a hemocytometer. The medium is added to the molasses alcohol wastewater:

[0030] (2) 60mL molasses alcohol wastewater liquid culture medium in 250mL Erlenmeyer flask, the culture medium is to dilute molasses alcohol wastewater to A 475 ≈3.5, plus glucose 25g / L, NaNO3 2.0g / L, KH 2 PO 4 1.0g / L, MgSO 4 ·7H 2 O 0.5g / L, adjust the pH to 5.0, and the spore inoculation amount is 10 4 cells / mL, 39°C, 150rpm shaking culture for ...

Embodiment 2

[0032] (1) Cultivation of bacteria seed liquid and preparation of inoculum are the same as step (1) of Example 1.

[0033] (2) 60mL molasses alcohol wastewater liquid culture medium in 250mL Erlenmeyer flask, the culture medium is to dilute molasses alcohol wastewater to A 475 ≈3.5, add starch 25g / L, NaNO 3 2.0g / L, KH 2 PO 4 1.0g / L, MgSO 4 ·7H 2 O 0.5g / L, adjust the pH to 5.0, and the spore inoculation amount is 10 5 cells / mL, 39°C, 150rpm shaking culture for 4 days, take out the shake flask, filter and separate the bacteria, take 1mL of the filtrate and dilute it 10 times with acetic acid-sodium acetate buffer (0.1M, pH5.0), and measure it with a visible spectrophotometer The change of absorbance at 475nm before and after decolorization. The bacteria were dried overnight at 105°C in an oven, and weighed with an electronic analytical balance. The decolorization rate was 58.09%, and the dry cell weight was 12.8067g / L.

Embodiment 3

[0035] (1) Cultivation of bacteria seed liquid and preparation of inoculum are the same as step (1) of Example 1.

[0036] (2) 60mL molasses alcohol wastewater liquid culture medium in 250mL Erlenmeyer flask, the culture medium is to dilute molasses alcohol wastewater to A 475 ≈3.5, add starch 30g / L, peptone 3.0g / L, KH 2 PO 4 1.0g / L, MgSO 4 ·7H 2 O 0.5g / L, adjust the pH to 5.0, and the spore inoculation amount is 10 5 cells / mL, 39°C, 150rpm shaking culture for 4 days, take out the shake flask, filter and separate the bacteria, take 1mL of the filtrate and dilute it 10 times with acetic acid-sodium acetate buffer (0.1M, pH5.0), and measure it with a visible spectrophotometer The change of absorbance at 475nm before and after decolorization. The bacteria were dried overnight at 105°C in an oven, and weighed with an electronic analytical balance. The decolorization rate was 62.67%, and the dry cell weight was 13.4083g / L.

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Abstract

The invention relates to a microorganism used for decolouring molasses alcohol waste water, namely aspergillus flavus, preserved in the China General Microbiological Culture Collection Center on November 1, 2010. During screening and separation of the aspergillus flavus, pigment melanoidins simulating molasses alcohol waste water is taken as a substrate, a dilution spread plate method is adopted for screening in natural environment to obtain the melanoidins decolouring microorganism, bacterial strains with an obvious decolouring ring are transferred into an untreated actual molasses alcohol waste water culture medium to carry out secondary screening, the bacterial strains with decolouring capability are transferred into a culture medium to be cultured, and spores are eluted to prepare spore suspension. The molasses alcohol waste water is diluted until A 475 is about 3.5, a nutrient source is added, the spore suspension is inoculated into the molasses alcohol waste water liquid culturemedium for carrying out decolouring reaction, after reaction is finished, thallus is leached and separated, and decolouring rate is calculated by testing variation of absorbance at 475nm.

Description

technical field [0001] The invention belongs to the field of wastewater biological treatment, in particular to a microorganism used for decolorizing molasses alcohol wastewater. [0002] The present invention also relates to a method for screening and isolating the above-mentioned microorganisms. [0003] The present invention also relates to a method for biological decolorization of molasses alcohol wastewater by using the above-mentioned microorganisms. Background technique [0004] Fermentation of molasses wastewater to produce alcohol has always been one of the important ways for the sugar industry to improve industry efficiency and solve molasses emissions. It can also meet the needs of future energy diversification brought about by the gradual depletion of petroleum energy. However, the high pigment value of molasses alcohol wastewater It is one of the "bottleneck" problems facing the development of this industry. The difficulty of processing lies in the fact that the...

Claims

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Application Information

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IPC IPC(8): C12N1/14C02F3/34C12R1/67
Inventor 刘幽燕李必金贺锴何熙璞李青云
Owner GUANGXI UNIV
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