Preparation method of nano-hydroxyapatite/chitosan

A nano-hydroxyapatite and chitosan technology, applied in chemical instruments and methods, phosphorus compounds, inorganic chemistry, etc., can solve problems such as lack, and achieve the effects of regular morphology, simple preparation process and good crystallinity

Inactive Publication Date: 2013-03-20
JIANGNAN UNIV
View PDF1 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there is still a lack of a convenient preparation method for the successful preparation of nano-hydroxyapatite/chitosan composite materials with particle width and

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of nano-hydroxyapatite/chitosan
  • Preparation method of nano-hydroxyapatite/chitosan
  • Preparation method of nano-hydroxyapatite/chitosan

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0022] Example 1: A method for preparing nano-hydroxyapatite / chitosan, including the following process steps:

[0023] (1) Synthesis of nano-hydroxyapatite: mix sodium phosphate solution, calcium chloride solution and cetyltrimethylammonium bromide solution (CTAB) to obtain a reaction system, in which Ca 2+ The ion concentration is 0.08mmol·L -1 , The concentration of phosphate ion is 0.048mmol·L -1 , The concentration of CTAB is 8×10 -4 mol·L -1 , And the molar ratio of calcium ions to phosphate ions is 5:3; react for 12 to 96 hours, and adjust the pH value to maintain 8; centrifuge the obtained product through a 0.22μm microporous membrane filter, the The filtered solid was washed alternately with water and absolute ethanol twice, and then dried in vacuum at 30°C overnight to obtain nano-hydroxyapatite powder;

[0024] (2) Dissolve 0.05 g of chitosan powder in 5 mL of 1% by mass acetic acid aqueous solution to obtain a chitosan solution;

[0025] (3) Weigh 0.05g of the nano-hydroxy...

Example Embodiment

[0029] Embodiment 2: A preparation method of nano-hydroxyapatite / chitosan, including the following process steps:

[0030] (1) Synthesis of nano-hydroxyapatite: mixing sodium hydrogen phosphate solution, calcium chloride solution and cetyltrimethylammonium bromide solution (CTAB) to obtain a reaction system, in which Ca 2+ The ion concentration is 500mmol·L -1 , The concentration of phosphate ion is 300mmol·L -1 , The concentration of CTAB is 10.5×10 -4 mol·L -1 , And the molar ratio of calcium ions to phosphate ions is 5:3; react for 96 hours, and adjust the pH value to maintain 14; centrifuge the obtained product through a 0.22μm microporous membrane filter, filter the product Wash the solid with water and absolute ethanol alternately for 20 times, and then vacuum-dry at 80°C overnight to obtain nano-hydroxyapatite powder;

[0031] (2) Dissolve 1.5g of chitosan powder in 500 mL of 5% by mass acetic acid aqueous solution to obtain a chitosan solution;

[0032] (3) Weigh 1.5g of the ...

Example Embodiment

[0033] Embodiment 3: A preparation method of nano-hydroxyapatite / chitosan, including the following process steps:

[0034] (1) Synthesis of nano-hydroxyapatite: Mix sodium dihydrogen phosphate solution, calcium chloride solution and cetyltrimethylammonium bromide solution (CTAB) to obtain a reaction system, in which Ca 2+ The ion concentration is 10 mmol·L -1 , The concentration of phosphate ion is 6 mmol·L -1 , The concentration of CTAB is 9×10 -4 mol·L -1 , And the molar ratio of calcium ions to phosphate ions is 5:3; react for 36 hours, and adjust the pH value to maintain 10; centrifuge the obtained product through a 0.22μm microporous membrane filter, filter the product Wash the solid with water and absolute ethanol alternately for 10 times, and then vacuum-dry at 50°C overnight to obtain nano-hydroxyapatite powder;

[0035] (2) Dissolve 1 g of chitosan powder in 250 mL of 2% acetic acid aqueous solution by mass percentage to obtain a chitosan solution;

[0036] (3) Weigh 1g of ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Widthaaaaaaaaaa
Lengthaaaaaaaaaa
Widthaaaaaaaaaa
Login to view more

Abstract

The invention relates to a preparation method of a nano-hydroxyapatite/chitosan composite material. The preparation method comprises the following steps of: uniformly mixing nano-hydroxyapatite with an acetic acid solution of chitosan, mixing a mixture with liquid paraffin, adding an emulsifying agent span80 (sorbitan oleate); after a system is fully emulsified, adding glutaraldehyde for carrying out crosslinking reaction; and centrifugally separating, washing and vacuum-drying a suspension and then collecting to obtain the nano-hydroxyapatite/chitosan composite material. According to the reversed-phase microemulsion preparation method of the nano-hydroxyapatite/chitosan composite material, provided by the invention, the nano-hydroxyapatite/chitosan composite material has the characteristics of particle width and long diameter within a nano scale range, regular feature and good crystallinity. The preparation method is simple in preparation process and low in cost, is suitable for batch production, and has an important application value in the field of biomedicine.

Description

technical field [0001] The invention relates to a preparation method of nano-hydroxyapatite / chitosan, belonging to the technical field of inorganic nano-materials. Background technique [0002] Nano-hydroxyapatite (n-HA) has good biocompatibility and bioactivity, and is widely used in bone tissue repair and replacement technology, but the material is too brittle, which limits its use in bone replacement and Application in bone repair. Chitosan (CS) is a polysaccharide with cations after deacetylation of chitin, which is naturally degradable, non-toxic, harmless, non-irritating to the human body and tissues, and has good biocompatibility. Adhesion and proliferation have many excellent properties such as promoting effect. If n-HA and CS are made into a composite material, the new material is expected to improve its mechanical properties in addition to its excellent biocompatibility, so there is a large room for development in the field of bone substitute materials. The rese...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C08J3/24C08J3/07C08L5/08C08K3/32C01B25/32
CPCC01B25/32C08J3/24C08L5/08C08K3/32C08J3/07C08J3/205C08J2305/08C08K2003/325C08K2201/011
Inventor 李玲董燕超方云王方育
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap