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Preparation method of redox mediator

A mediator, solid technology, applied in the field of catalyst synthesis, can solve the problems of small yield, difficult purification, poor water solubility, etc., and achieves the effects of low preparation cost, difficult photodegradation, and low price

Inactive Publication Date: 2014-08-06
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional redox mediators are mostly natural products with small yields, difficult purification and poor water solubility

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-3

[0024] In embodiment 1-3: riboflavin (C 17 h 20 N 4 o 6 ), dihydrate sulfosalicylic acid (C 7 h 6 o 6 S·2H 2 O), thionyl chloride (SOCl 2 ) and sodium bicarbonate (NaHCO 3 ) were purchased from Sinopharm Chemical Reagent Co., Ltd.

Embodiment 1

[0026] A preparation method of redox mediator, the steps are:

[0027] 1. Weigh 10.000g of sulfosalicylic acid dihydrate crystals and place them on a watch glass, put them in a vacuum drying oven, and dry them at 70°C to constant weight. Weigh 3.0000g of riboflavin solids and put them on a watch glass. Dry in a vacuum oven at 70°C to constant weight;

[0028] 2. Weigh 2.6090g of dried anhydrous sulfosalicylic acid solids and 1.0000g of riboflavin, mix them evenly, and place them in a 50mL round bottom flask;

[0029] 3. Measure 25 mL of thionyl chloride and add it to the mixture in step 2;

[0030] 4. Put the round bottom flask in a water bath and stir to reflux, control the reaction temperature not to exceed 40°C, stir for 12 hours, the mixture becomes an orange-red transparent solution;

[0031] 5. Pour the orange-red transparent solution obtained in step 4 into a petri dish, place in the air and blow out the remaining thionyl chloride to obtain an orange-red jelly;

[00...

Embodiment 2

[0040] A preparation method of redox mediator, the steps are:

[0041] 1, according to the step 1 of embodiment 1, dihydrate sulfosalicylic acid crystal and riboflavin are dried;

[0042] 2. Weigh 5.8610g of dried anhydrous sulfosalicylic acid solid and 2.2464g of riboflavin, mix them evenly, and place them in a 100mL round bottom flask;

[0043] 3. Measure 50 mL of thionyl chloride and add it to the mixture in step 2;

[0044] 4. Put the round bottom flask in a water bath and stir to reflux, control the reaction temperature not to exceed 40°C, stir for 12 hours, the mixture becomes an orange-red transparent solution;

[0045] 5. Pour the orange-red transparent solution obtained in step 4 into a petri dish, place in the air and blow out the remaining thionyl chloride to obtain an orange-red jelly;

[0046] 6. Add distilled water to dissolve the orange-red gel just completely, then add solid sodium bicarbonate to adjust the pH of the solution to pH7;

[0047] 7. Add 10 times...

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PUM

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Abstract

The invention belongs to the technical field of catalyst synthesis, and particularly discloses a preparation method of an isoalloxazine redox mediator. The synthesized redox mediator is good in water solubility and biocompatibility and can be applied to reaction of performing degradation on organic pollutants by selective catalytic microorganisms. The preparation method comprises the following specific steps of: 1, performing vacuum drying on sulfosalicylic acid dehydrate crystals and riboflavin; 2, evenly mixing the riboflavin and anhydrous sulfosalicylic acid; 3, adding thionyl chloride into the mixture; 4, stirring the mixture in a water bath for reflux reaction at a temperature of no more than 40 DEG C; 5, reacting until the mixture becomes orange red clear and transparent solution, and drying a solvent in air; 6, adding water into the produced orange red jelly for dissolving, and then adding sodium bicarbonate into the dissolved jelly to adjust the pH value of the dissolved jelly to pH7; 7, adding absolute ethyl alcohol into the solution to separate out orange-yellow solids, wherein the volume of the absolute ethyl alcohol is ten times that of the solution; and 8, filtering the solids, and repeatedly washing a filter cake with absolute ethyl alcohol for many times, and performing vacuum drying on the filter cake at 70 DEG C.

Description

technical field [0001] The invention relates to the technical field of synthesis of catalysts, and more specifically to a method for preparing an isoalloxazine redox mediator. The synthesized redox mediator has good water solubility and good biocompatibility, and can be used for selective Catalyzes the reaction of microorganisms to degrade organic pollutants. Background technique [0002] Mediator catalysis technology is a technology that uses a certain medium in the original reaction system, and the medium is reversibly oxidized and reduced between the primary electron donor and the final electron acceptor, thereby accelerating the electron transfer rate of the original reaction, thereby accelerating the reaction rate. . This technology can greatly increase the rate of biodegradation without producing by-products. Traditional redox mediators are mostly natural products with small yields, difficult purification and poor water solubility. Therefore, the chemical synthesis ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J31/02A62D3/02A62D101/20
Inventor 周培疆潘可亮
Owner WUHAN UNIV