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Preparation of electrochemical three-dimensional microfluidic paper chip with high flux, low cost and simplicity in operation, and application of electrochemical three-dimensional microfluidic paper chip to field test

A microfluidic paper chip, electrochemical technology, applied in the direction of material electrochemical variables, chemical instruments and methods, material analysis through electromagnetic means, etc., can solve the problems of false positive results, poor selectivity, and low sensitivity of colorimetry

Inactive Publication Date: 2013-04-17
UNIV OF JINAN
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the analysis methods based on microfluidic paper chip analysis devices are mainly colorimetric methods, because colorimetric methods can only give a "yes / no" signal response, and colorimetric methods have low sensitivity and poor selectivity , prone to false positive results

Method used

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  • Preparation of electrochemical three-dimensional microfluidic paper chip with high flux, low cost and simplicity in operation, and application of electrochemical three-dimensional microfluidic paper chip to field test
  • Preparation of electrochemical three-dimensional microfluidic paper chip with high flux, low cost and simplicity in operation, and application of electrochemical three-dimensional microfluidic paper chip to field test
  • Preparation of electrochemical three-dimensional microfluidic paper chip with high flux, low cost and simplicity in operation, and application of electrochemical three-dimensional microfluidic paper chip to field test

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Example 1 On-site rapid simultaneous detection of glucose, cholesterol, uric acid, and hemoglobin in human blood.

[0084] (1) Use Adobe Illustrator CS4 software on the computer to design three hydrophobic wax batch printing patterns of the electrochemical three-dimensional microfluidic paper chip, respectively wax batch printing pattern A (the style is as attached figure 1shown), wax batch printing pattern B (style as attached figure 2 shown), wax batch printing pattern C (style as attached image 3 shown). The paper channel on wax pattern A has a width of 2 mm and a length of 30 mm. The paper channel on wax pattern B has a width of 2 mm and a length of 12 mm. The diameter of the electrochemical detection hole on wax pattern C is 4 mm.

[0085] (2) Use Adobe Illustrator CS4 software on the computer to design the array working electrode batch printing pattern matching the wax batch printing pattern C (the style is as attached Figure 4 shown), each working elect...

Embodiment 2

[0112] Example 2 On-site rapid simultaneous detection of glucose, polyphenols, xanthines, cholesterol, uric acid, and heme in human urine.

[0113] "Utilize Adobe Illustrator CS4 software design" in embodiment 1 step (1) (2) (3) (4) (5) (6) (23) to " utilize Photoshop CS4 software design "; "Ordinary qualitative filter paper" in "ordinary qualitative filter paper" is changed to "ordinary quantitative filter paper"; "heating at 100°C for 1 minute" in step (9) of Example 1 is changed to "heating at 150°C for 30 seconds"; the embodiment 1 step 13) in the "gold nanoparticle solution" into "silver nanoparticle solution"; change the "three high patient blood sample" in Example 1 to "three high patient urine sample"; change the embodiment 1 step ( 27) Change to "Take 5 ml of the patient's urine sample, and use an ordinary plastic dropper to continuously drop it into the sampling hole of the electrochemical three-dimensional microfluidic paper chip. After 10 consecutive drops, turn ...

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Abstract

The invention discloses a preparation method of an electrochemical three-dimensional microfluidic paper chip with high flux, low cost and simplicity in operation, and an application method of the electrochemical three-dimensional microfluidic paper chip to a field test. According to the invention, hydrophobic wax patterns are printed on three pieces of common filter paper with the A4 size respectively in batches by a full-printing-level adhesion piling mode. The preparation process comprises the following steps: printing the hydrophobic wax patterns in batches; melting wax and forming; screen-printing an array working electrode, a reference electrode and a counter electrode on the filter paper in batches; printing horse radish peroxidase and oxidase on the surface, without the working electrode, of the filter paper sequentially in batches; cutting the electrochemical three-dimensional microfluidic paper chip; preparing a double-sided adhesive tape sheet; adhering the filter sheets through the double-sided adhesive tape sheet; and preparing an electrochemical three-dimensional microfluidic paper chip clip. A method for performing field test on the electrochemical three-dimensional microfluidic paper chip comprises the following steps: clamping the paper chip by using the electrochemical three-dimensional microfluidic paper chip clip; inserting into six copper finger slots; and connecting an electrochemical work station through a multiplexer.

Description

[0001] technical field [0002] The invention relates to the field of low-cost, high-throughput, high-sensitivity, high-specificity on-site instant detection technology, more specifically a construction of a microfluidic paper-on-a-chip laboratory suitable for high-throughput electrochemical enzyme analysis . Background technique [0003] Paper is a very cheap and abundant material, and test strips have also been widely used as an analysis platform (such as immunochromatography) for simple disease diagnosis and environmental testing, such as early pregnancy test strips, etc. . The Whiteside research group of the Harvard University School of Chemistry first proposed a new concept of a paper chip laboratory, also known as a microfluidic paper chip analysis device. The microfluidic paper chip analysis device refers to drawing hydrophobic patterns on paper. Since the paper has a strong hydrophilic ability, the part without hydrophobic patterns will form a hydrophilic channel / r...

Claims

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Application Information

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IPC IPC(8): B01L3/00G01N27/416G01N27/30
Inventor 于京华葛磊王盼盼颜梅葛慎光张彦楚成超李伟平苏敏王衍虎臧德进闫纪宪王少伟王寿梅张萌
Owner UNIV OF JINAN
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