Method for improving anaerobic digestion performance of sludge and synchronously enlarging cultivation by functional bacteria

A technology of anaerobic digestion and functional bacteria, which is applied in the field of environmental protection and comprehensive resource utilization, can solve the problems of obtaining competitive advantages, difficulty, and connection, and achieve the goals of improving anaerobic digestion efficiency, efficient white matter hydrolysis efficiency, and reducing treatment costs Effect

Active Publication Date: 2013-04-24
TONGJI UNIV
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AI Technical Summary

Problems solved by technology

[0006] It can be seen that the current biological bacteria pretreatment method is generally used for aerobic or facultative fermentation of sludge, and is rarely connected with anaerobic digestion of sludge; the biological bacteria used are aerobic or facultative bacteria, and it is difficult to Obtain a competitive advantage in the anaerobic environment of the sludge anaerobic digestion process, and the sludge anaerobic digestion reactor is easily replaced by the indigenous anaerobic bacteria in the reactor, which means that these biological bacteria can only be used for sludge Before anaerobic digestion, each feeding is a fresh bacterial agent that needs to be re-expanded and cultivated with a special medium, which greatly increases the cost of pretreatment; and this type of biological bacteria utilizes the relatively easily degradable organic matter in the sludge. However, it is difficult to strengthen the hydrolysis of a large amount of relatively refractory organic matter remaining in the late stage of sludge anaerobic digestion.

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  • Method for improving anaerobic digestion performance of sludge and synchronously enlarging cultivation by functional bacteria

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] like figure 1 Shown:

[0031] (1) Take 5 g of anaerobic digested biogas residues treated with feces, place them in 1L of gel yeast extract medium, seal and avoid light for anaerobic cultivation, and use N 2 The air drives away the oxygen, the culture temperature is controlled at 55°C, and the pH is controlled at 7.0; after 10 days of anaerobic culture, the protease activity is 116±20U / mL. The main composition of the gel yeast extract medium is (1L): 3.0g gel, 2.0g yeast extract, 2.0g trypticase casein, 1.0g NH4Cl, 1.0g MgCl2 6H2O, 0.4g CaCl2 2H2O, K2HPO4·3H2O 0.4g, NaHCO38.4g, Na2S·9H2O 0.5g, trace element solution 10.0ml, distilled water 1L.

[0032] (2) Take 50 mL of the bacterial solution containing functional bacteria obtained in step (1), add 1 L of gel yeast extract medium with the same composition as step (1) and seal it for anaerobic culture in the dark, and use N 2 The air was used to drive away the oxygen, the culture temperature was controlled at 55°C, and...

Embodiment 2

[0036] A method of using functional bacteria to improve sludge anaerobic digestion performance and simultaneously expand cultivation, the process flow is as follows figure 1 As shown, the sludge is hydrolyzed by methanogenic bacteria in the anaerobic digester to form methane and anaerobic digestion liquid, and the functional bacteria liquid is obtained after the functional bacteria are expanded and cultivated, or the functional bacteria liquid is mixed with the sludge digestion residue , inoculated into the anaerobic digestion solution, the refractory protein in the anaerobic digestion solution is hydrolyzed into small molecular organic matter, the methanogenic bacteria hydrolyze the small molecular organic matter to form methane, and the functional bacteria proliferate the cells themselves, and finally obtain rich Sludge digestion residues of methanogens and functional bacteria. The method includes the following steps:

[0037] (1) Collection and screening of functional bact...

Embodiment 3

[0042] A method for improving the anaerobic digestion performance of sludge by using functional bacteria and simultaneously expanding cultivation, comprising the following steps:

[0043] (1) Collection and screening of functional bacteria: Take the anaerobic digested biogas residue treated with sludge, place it in beef extract with only protein as the organic matter, and culture it in a sealed, dark-proof anaerobic culture. The culture conditions are: temperature 35°C, pH Under the condition of 6.5-7.5, anaerobic culture was carried out for 7 days to obtain a bacterial solution containing functional bacteria with protease activity not less than 100U / mL;

[0044] (2) Expanded cultivation of functional bacteria: take the bacterium liquid containing functional bacteria obtained in step (1), add the organic matter type only in the beef extract of protein, seal and avoid light for anaerobic culture, and its culture conditions are the same as those of step (1) The conditions are th...

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Abstract

The invention relates to a method for improving anaerobic digestion performance of sludge and synchronously enlarging cultivation by functional bacteria. The method comprises the following steps of: collecting and screening the functional bacteria; enlarging cultivation of the functional bacteria; preparing functional bacteria solution; inoculating the functional bacteria solution into an anaerobic digestion reactor containing sludge after carrying out anaerobic digestion for 10-15 days on the sludge, further carrying out anaerobic digestion on the sludge for 15-30 days, hydrolyzing protein which is difficult to degrade in the sludge into low molecular weight organic matters by the functional bacteria; hydrolyzing the low molecular weight organic matters to form methane by mathanogenic bacteria; and carrying out multiplication of bacteria cells by the functional bacteria, so as to finally obtain sludge digestion residue containing the methanogenic bacteria and the functional bacteria. Compared with the prior art, advantages enrichment and expansion of the functional bacteria are achieved by the method; the obtained sludge digestion residue contains target functional bacteria, and can be used as an inoculant to be applied to anaerobic digestion treatment of the sludge by circulating inoculation; and the anaerobic digestion efficiency of the sludge is lastingly improved, and the treatment cost is reduced.

Description

technical field [0001] The invention belongs to the fields of environmental protection and resource comprehensive utilization, and relates to a process for anaerobic digestion treatment of sludge, in particular to a method for improving the anaerobic digestion performance of sludge by using functional bacteria and simultaneously expanding cultivation. Background technique [0002] Sludge from urban sewage treatment plants is the main secondary pollutant produced by sewage treatment. It is estimated that the national dry sludge output will reach 7 million to 12 million tons per year during the 12th Five-Year Plan period. If such a huge amount of sludge is not properly disposed of, it will cause serious secondary pollution to the environment. Biological treatment of sludge includes aerobic treatment, fermentation and anaerobic digestion. Sludge anaerobic digestion is a sludge treatment process widely used at present. It refers to the degradation of carbohydrates, proteins, f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F11/04C12N1/20
CPCY02E50/30
Inventor 吕凡何品晶王天烽李天水邵立明
Owner TONGJI UNIV
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