Method for improving anaerobic digestion performance of sludge and synchronously enlarging cultivation by functional bacteria
A technology of anaerobic digestion and functional bacteria, which is applied in the field of environmental protection and comprehensive resource utilization, can solve the problems of obtaining competitive advantages, difficulty, and connection, and achieve the goals of improving anaerobic digestion efficiency, efficient white matter hydrolysis efficiency, and reducing treatment costs Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0030] like figure 1 Shown:
[0031] (1) Take 5 g of anaerobic digested biogas residues treated with feces, place them in 1L of gel yeast extract medium, seal and avoid light for anaerobic cultivation, and use N 2 The air drives away the oxygen, the culture temperature is controlled at 55°C, and the pH is controlled at 7.0; after 10 days of anaerobic culture, the protease activity is 116±20U / mL. The main composition of the gel yeast extract medium is (1L): 3.0g gel, 2.0g yeast extract, 2.0g trypticase casein, 1.0g NH4Cl, 1.0g MgCl2 6H2O, 0.4g CaCl2 2H2O, K2HPO4·3H2O 0.4g, NaHCO38.4g, Na2S·9H2O 0.5g, trace element solution 10.0ml, distilled water 1L.
[0032] (2) Take 50 mL of the bacterial solution containing functional bacteria obtained in step (1), add 1 L of gel yeast extract medium with the same composition as step (1) and seal it for anaerobic culture in the dark, and use N 2 The air was used to drive away the oxygen, the culture temperature was controlled at 55°C, and...
Embodiment 2
[0036] A method of using functional bacteria to improve sludge anaerobic digestion performance and simultaneously expand cultivation, the process flow is as follows figure 1 As shown, the sludge is hydrolyzed by methanogenic bacteria in the anaerobic digester to form methane and anaerobic digestion liquid, and the functional bacteria liquid is obtained after the functional bacteria are expanded and cultivated, or the functional bacteria liquid is mixed with the sludge digestion residue , inoculated into the anaerobic digestion solution, the refractory protein in the anaerobic digestion solution is hydrolyzed into small molecular organic matter, the methanogenic bacteria hydrolyze the small molecular organic matter to form methane, and the functional bacteria proliferate the cells themselves, and finally obtain rich Sludge digestion residues of methanogens and functional bacteria. The method includes the following steps:
[0037] (1) Collection and screening of functional bact...
Embodiment 3
[0042] A method for improving the anaerobic digestion performance of sludge by using functional bacteria and simultaneously expanding cultivation, comprising the following steps:
[0043] (1) Collection and screening of functional bacteria: Take the anaerobic digested biogas residue treated with sludge, place it in beef extract with only protein as the organic matter, and culture it in a sealed, dark-proof anaerobic culture. The culture conditions are: temperature 35°C, pH Under the condition of 6.5-7.5, anaerobic culture was carried out for 7 days to obtain a bacterial solution containing functional bacteria with protease activity not less than 100U / mL;
[0044] (2) Expanded cultivation of functional bacteria: take the bacterium liquid containing functional bacteria obtained in step (1), add the organic matter type only in the beef extract of protein, seal and avoid light for anaerobic culture, and its culture conditions are the same as those of step (1) The conditions are th...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com