Method for utilizing covalence coupling to prepare photon point-antibody compound

An antibody complex and covalent coupling technology, which can be used in measurement devices, instruments, scientific instruments, etc., can solve the problems of easy precipitation and false positives in immunodetection, and achieves simple and easy method, improved colloid stability, The effect of improving immune properties

Inactive Publication Date: 2013-04-24
韩焕兴 +2
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, in the current methods for preparing quantum dot-antibody complexes by covalent coupling, blocking agents are rarely used after quantum dots are covalently coupled to antibodies, or non-covalent methods are used to block the surface of quantum dots, and blocking agents have no effe

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  • Method for utilizing covalence coupling to prepare photon point-antibody compound
  • Method for utilizing covalence coupling to prepare photon point-antibody compound

Examples

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Example Embodiment

[0025] Example 1

[0026] Preparation of quantum dots:

[0027] (1) Mix 1 millimoles of cadmium oxide, 4 millimoles of stearic acid and 10 ml of liquid paraffin, and then heat to 200°C under the protection of nitrogen until the cadmium oxide is completely dissolved; then the system is cooled to room temperature, and 10 ml of liquid paraffin is added. 1 g of trioctyl phosphine oxide and 3 g of octadecyl amine, heated to 300 ℃ under nitrogen protection; 4 millimoles of selenium powder was dissolved in 5 ml of liquid paraffin and 1 ml of trioctyl phosphine, and quickly injected into the above cadmium solution, reaction After 30 minutes, CdSe quantum dots with a particle size of about 4 nanometers are obtained;

[0028] (2) At 250°C, add 2 ml of 0.1 mM zinc stearate paraffin solution to the CdSe quantum dot system prepared in step (1), and after reacting for 10 minutes, add 2 ml of 0.1 mM sulfur powder paraffin solution and react for 10 minutes; Repeat the above steps (that is, the rea...

Example Embodiment

[0031] Example 2

[0032] Preparation of quantum dots:

[0033] (1) 0.68 g of cadmium chloride and 1.20 g of glutathione are dissolved in 5 ml of deionized water and adjusted to alkaline (pH=10.0);

[0034] (2) Mix 0.08 g of selenium powder and 0.39 g of sodium borohydride, inject 5 ml of deionized water, and react for 30 minutes to obtain a sodium selenium hydride solution;

[0035] (3) Mix the solutions obtained in steps (1) to (2), and heat the reaction kettle at 140° C. for 60 minutes under the protection of nitrogen to obtain CdTe water-soluble quantum dots with a particle size of about 5 nanometers.

[0036] Disperse 5 nanomoles of the prepared CdTe quantum dots (particle size: about 5 nanometers) with carboxyl groups on the surface in 1 ml of 100 mM phosphate buffer (pH 7.4), and then add EDC 0.25 micromolar and NHS 0.25 respectively. Micromolar, and then incubate the reaction on a shaker for 2 hours at room temperature to activate the carboxyl groups on the surface of the quant...

Example Embodiment

[0037] Example 3

[0038] Preparation of quantum dots:

[0039] (1) Mix 1 mmol of cadmium oxide, 4 mmol of stearic acid and 10 ml of liquid paraffin, and then heat to 200°C under nitrogen protection until the cadmium oxide is completely dissolved; then the system is cooled to room temperature, and 10 ml of liquid paraffin is added. 1 g of trioctyl phosphine oxide and 3 g of octadecyl amine, heated to 300 ℃ under nitrogen protection; 4 millimoles of selenium powder was dissolved in 5 ml of liquid paraffin and 1 ml of trioctyl phosphine, and quickly injected into the above cadmium solution, reaction After 30 minutes, CdSe quantum dots with a particle size of about 4 nanometers were obtained.

[0040] (2) At 250°C, add 2 ml of 0.1 mM zinc stearate paraffin solution to the CdSe quantum dot system prepared in step (1), and after reaction for 10 minutes, add 2 ml of 0.1 mM selenium powder paraffin solution for 10 minutes; Repeat the above steps (that is, the reaction of adding zinc stear...

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Abstract

The invention discloses a method for utilizing covalence coupling to prepare a photon point-antibody compound. The method comprises the following steps of: after a photon point and antibody numerators are subjected to covalence coupling, sealing the unoccupied site of the surface of the photon point by use of a sealant, and connecting the sealant with an activating functional group on the surface of the photon point through a covalent bond, thereby forming the photon point-antibody compound. The method provided by the invention can keep a free space constellation of antibody numerators, the colloid stability of the nanometer particles is improved, the method provided by the invention is utilized to prepare the photon point-antibody compound, and the immunity detective sensitivity is improved; and the operation is simple and convenient, the immunity characteristic of the photon point-antibody compound is effectively improved, and the photon point is pushed to be applied on an immunity detection aspect.

Description

technical field [0001] The invention relates to a method for preparing a quantum dot-antibody complex, in particular to a method for preparing the quantum dot-antibody complex through covalent coupling. Background technique [0002] Quantum Dots (QDs) are semiconductor nanocrystals. Due to the size effect and quantum confinement effect of nanomaterials, they have some unique fluorescence properties, such as controllable fluorescence emission wavelength, narrow and symmetrical emission peak, and wide excitation wavelength range. , high quantum efficiency, good photostability, etc. Therefore, quantum dots have attracted widespread attention as an ideal fluorescent reporter group for immunoassays. [0003] Preparation of quantum dot-antibody (QD-Ab) complexes with high fluorescence intensity and good immunoactivity is the key to applying quantum dots to fluorescent immunoassays. The fluorescence intensity of the QD-Ab complex is determined by the fluorescence properties of the...

Claims

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Application Information

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IPC IPC(8): G01N33/531
Inventor 韩焕兴张鹏飞叶伟民
Owner 韩焕兴
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