Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for utilizing covalence coupling to prepare photon point-antibody compound

An antibody complex and covalent coupling technology, which can be used in measurement devices, instruments, scientific instruments, etc., can solve the problems of easy precipitation and false positives in immunodetection, and achieves simple and easy method, improved colloid stability, The effect of improving immune properties

Inactive Publication Date: 2013-04-24
韩焕兴 +2
View PDF5 Cites 19 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, in the current methods for preparing quantum dot-antibody complexes by covalent coupling, blocking agents are rarely used after quantum dots are covalently coupled to antibodies, or non-covalent methods are used to block the surface of quantum dots, and blocking agents have no effect on quantum dots. The influence of the dot-antibody complex performance is rarely reported. The blocking agent can effectively inhibit the aggregation of the particles after coupling the antibody, and it is easy to precipitate, and the immunoassay is prone to false positives and other problems.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for utilizing covalence coupling to prepare photon point-antibody compound
  • Method for utilizing covalence coupling to prepare photon point-antibody compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Preparation of quantum dots:

[0027] (1) Mix 1 mmol of cadmium oxide, 4 mmol of stearic acid and 10 milliliters of liquid paraffin, then heat to 200° C. under nitrogen protection until the cadmium oxide is completely dissolved; then the system is cooled to room temperature, and 10 milliliters of liquid paraffin, 1 gram of trioctylphosphine oxide and 3 grams of octadecylamine were heated to 300°C under nitrogen protection; 4 millimoles of selenium powder was dissolved in 5ml of liquid paraffin and 1ml of trioctylphosphine, and quickly injected into the above cadmium solution, the reaction After 30 minutes, CdSe quantum dots with a particle size of about 4 nanometers were obtained;

[0028] (2) At 250°C, add 2ml of 0.1mM zinc stearate paraffin solution to the CdSe quantum dot system prepared in step (1), react for 10min, then add 2ml of 0.1mM sulfur powder paraffin solution, and react for 10min; Repeat above-mentioned steps (namely add the reaction of zinc stearate para...

Embodiment 2

[0032] Preparation of quantum dots:

[0033] (1) 0.68 grams of cadmium chloride and 1.20 grams of glutathione were dissolved in 5 milliliters of deionized water, adjusted to alkaline (pH=10.0);

[0034] (2) Mix 0.08 gram of selenium powder and 0.39 gram of sodium borohydride, inject 5 ml of deionized water, react for 30 minutes, and obtain sodium selenium hydride solution;

[0035] (3) The solutions obtained in steps (1)-(2) were mixed, sealed in a reaction kettle under nitrogen protection, and heated at 140° C. for 60 minutes to obtain CdTe water-soluble quantum dots with a particle size of about 5 nanometers.

[0036] Disperse 5 nanomoles of the CdTe quantum dots (particle size about 5 nanometers) synthesized by the surface with carboxyl groups prepared above in 1 ml of 100 mM phosphate buffer (pH 7.4), and then add 0.25 micromoles of EDC and 0.25 micromoles of NHS Micromole, then at room temperature, incubated on a shaker for 2 hours to activate the carboxyl groups on the ...

Embodiment 3

[0038] Preparation of quantum dots:

[0039] (1) Mix 1 mmol of cadmium oxide, 4 mmol of stearic acid and 10 milliliters of liquid paraffin, then heat to 200° C. under nitrogen protection until the cadmium oxide is completely dissolved; then the system is cooled to room temperature, and 10 milliliters of liquid paraffin, 1 gram of trioctylphosphine oxide and 3 grams of octadecylamine were heated to 300°C under nitrogen protection; 4 millimoles of selenium powder was dissolved in 5ml of liquid paraffin and 1ml of trioctylphosphine, and quickly injected into the above cadmium solution, the reaction After 30 minutes, CdSe quantum dots with a particle size of about 4 nanometers were obtained.

[0040] (2) At 250° C., add 2 ml of 0.1 mM zinc stearate paraffin solution to the CdSe quantum dot system prepared in step (1), react for 10 min, then add 2 ml of 0.1 mM selenium powder paraffin solution, and react for 10 min; Repeat above-mentioned steps (namely add the reaction of zinc ste...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for utilizing covalence coupling to prepare a photon point-antibody compound. The method comprises the following steps of: after a photon point and antibody numerators are subjected to covalence coupling, sealing the unoccupied site of the surface of the photon point by use of a sealant, and connecting the sealant with an activating functional group on the surface of the photon point through a covalent bond, thereby forming the photon point-antibody compound. The method provided by the invention can keep a free space constellation of antibody numerators, the colloid stability of the nanometer particles is improved, the method provided by the invention is utilized to prepare the photon point-antibody compound, and the immunity detective sensitivity is improved; and the operation is simple and convenient, the immunity characteristic of the photon point-antibody compound is effectively improved, and the photon point is pushed to be applied on an immunity detection aspect.

Description

technical field [0001] The invention relates to a method for preparing a quantum dot-antibody complex, in particular to a method for preparing the quantum dot-antibody complex through covalent coupling. Background technique [0002] Quantum Dots (QDs) are semiconductor nanocrystals. Due to the size effect and quantum confinement effect of nanomaterials, they have some unique fluorescence properties, such as controllable fluorescence emission wavelength, narrow and symmetrical emission peak, and wide excitation wavelength range. , high quantum efficiency, good photostability, etc. Therefore, quantum dots have attracted widespread attention as an ideal fluorescent reporter group for immunoassays. [0003] Preparation of quantum dot-antibody (QD-Ab) complexes with high fluorescence intensity and good immunoactivity is the key to applying quantum dots to fluorescent immunoassays. The fluorescence intensity of the QD-Ab complex is determined by the fluorescence properties of the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/531
Inventor 韩焕兴张鹏飞叶伟民
Owner 韩焕兴
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com