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Lactobacillus casei and culture medium thereof

A technology of Lactobacillus casei and culture medium, applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., can solve problems such as arteriosclerosis and lower cholesterol

Inactive Publication Date: 2013-05-22
哈尔滨美华生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] (4) Cholesterol-lowering effect Cholesterol plays an important physiological role in the human body, but high serum cholesterol levels can easily induce arteriosclerosis, leading to a series of cardiovascular diseases
Existing vaccines and antibiotics often only treat diseases caused by certain known pathogenic microorganisms, but are powerless against their variants

Method used

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  • Lactobacillus casei and culture medium thereof
  • Lactobacillus casei and culture medium thereof
  • Lactobacillus casei and culture medium thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0021]The object of the present invention is to provide a kind of separation and cultivation method of Lactobacillus casei (Lactobacillus casei L7-13), the object of the present invention is achieved in this way: the percentages involved in the present invention are all percentages by weight.

[0022] 1. First collect samples from nature: Sterilize the sampling tools and various sampling containers. The sampling location is selected in the sludge of the sewer near the dairy production plant, in the soil of the milking farm, and in the stacked fresh solid feed In the layer, multi-point sampling is performed.

[0023] 2. Dilute the collected samples: mix the collected samples evenly under sterile conditions, weigh 20 grams of samples, dissolve them in 100 mL of sterile water, which contains 80-100 glass beads, and shake vigorously for 10 minutes , stand still for one minute, draw 1mL supernatant with a 1mL sterile pipette, transfer to a 9mL sterile water test tube, shake thoroug...

Embodiment 2

[0046] Culture method example one: the cultivation of Lactobacillus casei (Lactobacillus casei): Lactobacillus casei (Lactobacillus casei) L7-13 is cultivated, medium formula: solvent is water, glucose 10.0g / L, fructo-oligosaccharide 10.0g / L , xylooligosaccharide 10.0g / L, soybean peptone 10.0g / L, yeast extract powder 10.0g / L, beef powder 5.0g / L, dipotassium hydrogen phosphate 0.5g / L, trisodium citrate 0.5g / L, Ammonium citrate 0.1g / L, MgSO 4 ·7H 2 O 0.1g / L, MnSO 4 4H 2 O 0.1g / L, Tween 80 0.1mL / L; cultured for 36 hours at pH 4.5 and temperature 30°C, the bacteria grew well. The bacteria are collected by centrifugation above 4000rpm to obtain wet bacteria sludge, and then freeze-dried to obtain freeze-dried bacteria powder.

Embodiment 3

[0048] Culture method example two: the cultivation of Lactobacillus casei (Lactobacillus casei): Lactobacillus casei (Lactobacillus casei) L7-13 is cultivated, medium formula: solvent is water, glucose 45.0g / L, fructo-oligosaccharide 30.0g / L , xylooligosaccharide 30.0g / L, soybean peptone 20.0g / L, yeast extract powder 30.0g / L, beef powder 20.0g / L, dipotassium hydrogen phosphate 2.0g / L, trisodium citrate 2.0g / L, Ammonium citrate 2.0g / L, MgSO 4 ·7H 2 1.0g / L, MnSO 4 4H 2 0.5g / L, Tween 80 10mL / L; culture at pH 5.5 and temperature 35°C for 28 hours and centrifuge at 5000rpm for 15min to collect the bacteria to obtain wet sludge, and then freeze-dry to obtain freeze-dried bacteria powder.

[0049] Culture method example three: the cultivation of Lactobacillus casei (Lactobacillus casei): Lactobacillus casei (Lactobacillus casei) L7-13 is cultivated, medium formula: solvent is water, glucose 80.0g / L, fructo-oligosaccharide 50.0g / L , xylooligosaccharide 50.0g / L, soybean peptone 30...

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Abstract

The invention discloses a lactobacillus casei and a culture medium thereof. The bacterial strain is stored in China General Microbiological Culture Collection Center with the registration number of CGMCC NO. 3993; the Latin name of the strain is Lactobacillus casei L7-13; and the bacterial strain has the morphological characteristics of gram positive bacteria, no spore grown, no flagella, no motion, rough bacterial colony, offwhite or light yellow. The invention also discloses the complete sequence of 16s ribosome DNA (16S rDNA) of the bacteria; the lactobacillus casei is high in lactic acid generation speed and high in acid productivity, and further high in reproduction speed, high in activity and good in stress resistance; besides, the lactobacillus casei has the function of improving the gastrointestinal tract and thereby is capable of inhibiting colonization of harmful bacteria; the lactobacillus casei is capable of generating bile salt hydrolase and decomposing bound cholic acid into free cholic acid; and, the free cholic acid and cholesterol can form a composite so as to settle down together; therefore, the lactobacillus casei is capable of reducing cholesterol.

Description

technical field [0001] The invention provides a kind of lactobacillus casei (Lactobacillus casei L7-13) and culture medium thereof. Background technique [0002] Lactobacillus casei (Lactobacillus casei L7-13) belongs to the genus Lactobacillus, is a gram-positive bacterium that does not produce spores, has no flagella, and does not move; it is facultatively anaerobic, sometimes slightly aerobic, and grows in the presence of oxygen Poor, grows better when oxygen partial pressure is lowered; facultative heterofermentation of lactose, no liquefaction of gelatin, no reduction of nitrate, negative for catalase and oxidase; optimum growth temperature is 37°C, G+C content is 45.6% ~47.2%; the length of the bacteria is different, the two ends are square, and often form a chain; the colony is rough, off-white, sometimes slightly yellow, and can ferment various sugars. Lactobacillus casei is widely distributed and usually exists in human mouth, intestinal contents, stool and vagina....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/245
Inventor 梁金钟肖文娟
Owner 哈尔滨美华生物技术股份有限公司
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