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Piperazine degraded paracoccus PQ-01 and application thereof

A paracoccus and bacterial agent technology, applied in the field of environmental microorganisms, can solve the problem of no piperazine, etc., and achieve the effects of low production cost, good removal effect, and reduced wastewater treatment cost.

Active Publication Date: 2014-11-05
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Biodegradation is the main way to remove amines in nature. At present, there is no research report on piperazine-degrading bacteria in China. Therefore, microbial strains that efficiently degrade piperazine are isolated from piperazine-contaminated environments, and the use of microorganisms to restore Piperazine pollution in the environment has great theoretical significance and potential application prospects

Method used

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  • Piperazine degraded paracoccus PQ-01 and application thereof
  • Piperazine degraded paracoccus PQ-01 and application thereof
  • Piperazine degraded paracoccus PQ-01 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Isolation and identification of piperazine-degrading bacteria PQ-01

[0028] Take the piperazine-enriched bacterial solution and perform gradient dilution. The diluted solution is spread on the inorganic salt solid medium containing 100mg / L piperazine, and the medium formula is: every liter contains 1.5 g K 2 HPO 4 , 0.5 g KH 2 PO 4 , 0.2 g MgSO 4 ×7H 2 O, 1.0 g NaCl, 18 g agar, pH = 7.0, culture at 30°C for several days. A single colony was picked from it, and its degradation effect was verified by HPLC, and a strain with higher degradation efficiency was stored in a -20°C refrigerator with 30% glycerol. See the picture of its colony figure 1 , identified as Paracoccus ( Paracoccus sp.); named: PQ-01. Its crystal violet staining photo is as figure 2 As shown, the main biological characteristic is G - , In the logarithmic growth phase, the bacteria are spherical or club-shaped, without spores, and without motility. It can grow with piperazine as ...

Embodiment 2

[0029] Embodiment 2 laboratory biodegradation experiment

[0030] 1. Effect of Temperature on the Degradation Efficiency of Piperazine

[0031] In nitrogen-free basal salt medium (NaCl 1.0 g L -1 , K 2 HPO 4 1.5 g·L -1 , KH 2 PO 4 0.5 g L -1 , MgSO 4 ·7H 2 O 0.2 g L -1 , pH 7.0, the same below) by adding 100mg·L -1 piperazine, with 1% inoculum into the degrading strain seed liquid (OD 600 ≈1.6), at different temperatures (15°C, 25°C, 30°C, 35°C, 40°C), shaker 160r min -1 After culturing, samples were taken after 24 hours to measure the concentration of piperazine. The result is as Figure 4 shown. It shows that the optimum degradation temperature of PQ-01 is 30℃; it still has a certain degradation ability at lower temperature, but too low or too high ambient temperature will inhibit the biodegradation of piperazine.

[0032] 2. The effect of pH on the degradation efficiency of piperazine

[0033] Add 100mg L -1 piperazine, with 1% inoculum into the degrad...

Embodiment 3

[0034] Example 3 The method for the production of bacterial agent by piperazine-degrading bacteria PQ-01

[0035]Activate the original species of the piperazine-degrading bacteria PQ-01 of the present invention on a petri dish or in an incubator, at 30°C for 30 hours, and measure the degradation performance, and inoculate it on the inclined surface of the test tube after the mature colony. The test tube seed was inoculated into a 1000ml shake flask containing 200ml LB medium, the formula of LB medium g / L: yeast extract 5.00, peptone 10.00, NaCl 10.00, pH 7.0, constant temperature shaking culture to the logarithmic phase, ready to inoculate the seed tank. The seed tank is 500 liters, the feeding volume is 400 liters, the medium formula is: glucose 0.8%, (NH 4 ) 2 SO 4 1%, K 2 HPO 4 0.2%, MgSO 4 0.05%, NaCl 0.01%, CaCO 3 0.3%, yeast extract 0.02%, pH 7.2-7.5. After the feeding is completed, 121°C high-pressure damp heat sterilization, after cooling to 33°C, inoculate ...

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Abstract

The invention provides a degradation microbial inoculum for eliminating piperazine residue, and belongs to the high technical field of biology. The strain is gram staining reaction negative strain PQ-01, and is identified to be paracoccus sp., wherein the main biological characteristics are gram negative, spherical or club shape, asporous and non-motile. The strain can grow by taking piperazine as the unique carbon source and nitrogen source, and can be completely mineralized. 100mg / L of piperazine can be degraded to a level which cannot be detected in 24 hours by the strain under a shaking condition in the lab, so that the problem that the piperazine is difficult to biodegrade in waste water treatment can be solved.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms, in particular to a strain of piperazine-degrading paracoccus PQ-01 and its application. Background technique [0002] Amine compounds can be regarded as the products obtained after the hydrogen atoms in the ammonia molecule are replaced by hydrocarbon groups. Many amine compounds are widely used in medicine, industry, agriculture and other fields. Extensive use of organic amines will inevitably pollute the environment and endanger human health. Lower amines have a strong ammonia-like odor, while trimethylamine has an unpleasant fishy smell. Lower aliphatic amines are easily soluble in water, and their solubility and odor in water decrease with the increase of molecular weight. Aromatic amines are liquids or solids with an unpleasant odour. Arylamines with simpler structures are slightly soluble in water, and more complex ones are insoluble. Aromatic amines are highly tox...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/38
Inventor 蔡舒陈立伟蔡天明李欣胡江杨倩邱沪生
Owner NANJING AGRICULTURAL UNIVERSITY
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