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Method for extracting microalgae by utilizing dual-solvent system and obtaining extract

A dual-solvent and extract technology is applied in the new energy field of microalgae preparation of biodiesel technology, which can solve the problems of incapable of comprehensive utilization of microalgae resources, high solvent toxicity, unfavorable environmental protection, etc., and achieves less demanding equipment requirements, solvent safety, temperature Moderate effect

Inactive Publication Date: 2013-07-03
黄俊超
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the extraction method of microalgae in the prior art has the disadvantages of high solvent toxicity, serious pollution, and high cost, and the extraction method of microalgae in the prior art only extracts and utilizes the oil of microalgae, and other by-products and organic solvents are discarded. No, microalgae resources cannot be effectively and comprehensively utilized, which is not conducive to environmental protection

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  • Method for extracting microalgae by utilizing dual-solvent system and obtaining extract

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Example 1 investigates the efficiency of the process steps on the extraction of chlorella.

[0035] Chlorella is currently the most widely cultivated engineering algae species. Its cell wall is thicker than that of some other algae species, and it is more difficult to handle, so it is implemented as a model. The chlorella used was provided by Jiaxing Zeyuan Biological Products Co., Ltd. The specific steps are as follows:

[0036] a. First, the microalgae is subjected to a centrifugal treatment step, and the dry algae powder is obtained after spray drying;

[0037] b. Take 20 g of dry algae powder and add 300 ml of non-polar organic solvent petroleum ether to it, place the mixed solution of algae powder and non-polar organic solvent in a stirring tank, and stir vigorously at a speed of 500 r / min. Set as 60 minutes, the stirring temperature is set at 50°C;

[0038] c. Then, add 300 ml of polar organic solvent ethanol to the mixed solution treated in step b, place it in ...

Embodiment 2

[0045] Example 2 investigates the efficiency of the process steps on the extraction of Dunaliella salina.

[0046] The oil content of Dunaliella salina is relatively high. The Dunaliella salina used is provided by the Wuhan Institute of Hydrology, Chinese Academy of Sciences. The specific steps are as follows:

[0047] a. First, the microalgae is subjected to a centrifugal treatment step, and the dry algae powder is obtained after spray drying;

[0048] b. Take 5 g of dry algae powder and add 100 ml of non-polar organic solvent petroleum ether to it, place the mixed solution of algae powder and non-polar organic solvent in a stirring tank, and stir vigorously at a speed of 300 r / min. Set as 45 minutes, the stirring temperature is set at 50°C;

[0049] c. Then add 100ml of polar organic solvent ethanol to the mixed solution treated in step b, and place it in a stirring tank, stir at a speed of 300r / min, the stirring time is 45 minutes, and the stirring temperature is set at 50...

Embodiment 3

[0056] Example 3 investigates the efficiency of the process steps on the extraction of spirulina.

[0057] Spirulina has a high protein content and is widely used. The spirulina used was provided by Shandong Binzhou Tianjian Biotechnology Co., Ltd. The specific steps are as follows:

[0058] a. First, the microalgae is subjected to a centrifugal treatment step, and the dry algae powder is obtained after spray drying;

[0059] b. Take 20g of dry algae powder and add 400ml of non-polar organic solvent petroleum ether to it, place the mixed solution of algae powder and non-polar organic solvent in a stirring tank, and stir vigorously at a speed of 350r / min. Set as 45 minutes, the stirring temperature is set at 40°C;

[0060] c. Then add 400ml of polar organic solvent ethanol to the mixed solution treated in step b, place it in a stirring tank, stir at a speed of 350r / min, stir for 45 minutes, and set the stirring temperature at 40°C;

[0061] d. Feed acquisition step: leave th...

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Abstract

The invention discloses a method for extracting microalgae by utilizing a dual-solvent system and obtaining extract. The method comprises the following steps: centrifugalization, collection and spray drying of the microalgae are carried out, non-polar organic solvent such as light petroleum is added into the microalgae, the mixture of the microalgae and the light petroleum is poured into an agitation pot for agitation, and a polar organic solvent such as alcohol is added into the mixture for substance extraction. After solid-liquid separation, the obtained solid phase is dried, stored and used for the raw material of forage; the supernatant liquid is combined and mixed with water lotion, after standing, the obtained lower aqueous phase is added into calcium sulfate to precipitate the protein component thereof, and other liquid phase is used for recovering organic solvent. The method utilizes the resource of the microalgae to extract crude proteins and oil, and residual substances can be used as animal forage or fertilizer. The extract and separation method is simple, the extraction efficiency is high, requirements on equipment are low, the process enlargement is facilitated, both the selected non-polar and the polar solvents can be recovered and reused, consumption and pollution are avoided, and potential safety hazards of operators are prevented.

Description

technical field [0001] The invention belongs to the new energy field of biodiesel preparation technology from microalgae, and specifically relates to a comprehensive utilization method for extracting microalgae and its extract by using a dual-solvent system. Background technique [0002] The development of renewable new energy is a hot spot in the world today, and biomass energy is an important aspect of it. Biomass is formed by photosynthesis of organisms, absorbs and utilizes carbon dioxide, reduces the greenhouse effect, and is conducive to the recycling of carbon. There are many kinds of microalgae, widely distributed, fast growth and reproduction, higher light energy utilization efficiency, no land occupation, strong environmental adaptability, and it is a promising biomass energy. The oil content in some microalgae is high, up to 20-40%, so it is considered to be an important raw material for future bioenergy supply. However, the extraction method of microalgae in th...

Claims

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Application Information

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IPC IPC(8): C11B1/00C07K1/30A23K1/14C05F5/00
CPCY02A40/20
Inventor 黄俊超官芸杨洁王涛
Owner 黄俊超