Amphipathic carboxymethyl glucan-modified magnetic lipidosome, and preparation and application methods thereof
A technology of carboxymethyl dextran and magnetic liposomes, applied in the field of nanometers
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Embodiment 1
[0047] Preparation of SPIONs nanoparticles
[0048] Separately prepare 1M Fe 3+ solution (40mL) and 2M Fe 2+ solution (10 mL). The two solutions were added to 0.7M ammonia solution (250mL) at the same time, stirred by magnetic force, and the solution turned brown. After 30 minutes, adjust the pH value of the mixture with ammonia water until the pH is 10-11. During this process, the mixture gradually changes from brown to black, and black precipitates are formed. Continue to stir for 30 minutes. After stirring, put the mixed solution on a magnet for magnetic separation, and pour off the supernatant after the precipitate is completely settled. In order to obtain nanoparticles with good stability and single dispersion, we then modified the precipitate with perchloric acid and sodium citrate. Disperse the precipitate obtained in the previous step in 2M perchloric acid solution (100 mL), and stir magnetically for 30 min. Similarly, magnetic separation was performed again, the ...
Embodiment 2
[0050] Synthesis of Amphiphilic Carboxymethyl Dextran
[0051] Weigh 2.0g (equivalent to 12mmol of glucose units) of dextran T-10 and dissolve it in 2.0M NaOH solution (50mL); add 0.1M bromoacetic acid (10mL) to the magnetic stirring system, and react at room temperature (25°C) 24h. After the reaction, precipitate with ice ethanol, dissolve with a small amount of water after centrifugation; then put the solution into a dialysis bag with a molecular weight cut off of 3500, and dialyze with water, 0.1M HCl solution and water for 24 hours respectively. White flocculent CMD was obtained after freeze-drying. Weigh 0.3g of flocculent CMD and 0.02g of LiCl, dissolve in 20mL of DMF, stir magnetically and heat to 80°C to dissolve completely. After cooling to room temperature, add NHS / EDC (0.1M / 0.4M) to activate at room temperature for 10min. Then weigh 0.5 g of oleylamine and dissolve it in DMF, then add it dropwise into the activated CMD solution, and react at room temperature for ...
Embodiment 3
[0053] Preparation of amphiphilic carboxymethyl dextran-modified magnetic liposomes (CMD-MLs)
[0054] First, weigh soybean lecithin, cholesterol, and amphiphilic CMD according to the ratio of soybean lecithin / cholesterol / amphiphilic CMD=55 / 40 / 0.5 (mol / mol / mol), and dissolve soybean lecithin and cholesterol in 4 mL of methanol / chloroform (v / v=1 / 3) mixed organic solvent, amphiphilic CMD was dissolved in 4mL methanol / chloroform (v / v=1 / 1) mixed organic solvent, after complete dissolution, the two solutions Transfer to a 50mL flask, and evaporate at 37°C for 2h to remove the organic solvent, so that the phospholipid film is evenly dispersed on the bottle wall. Subsequently, with N 2 Blow dry repeatedly to blow away residual organic solvent. Finally, it was placed in a vacuum drying oven and dried for 3 hours to further remove the organic solvent. Add 10 mL of SPIONs-containing aqueous solution (0.5 mg / mL) for hydration, shake, and after the phospholipid film falls off complete...
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