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Preparation method and application of feeding bacillus licheniformis inoculant

A technology of Bacillus licheniformis and inoculum, which is applied in the field of feed probiotics, can solve the problems of slow development of the forage microecological inoculum industry, difficult control of bacterial content, and insignificant actual effects, etc. The effect of improving the utilization rate of digestion and low cost of use

Inactive Publication Date: 2013-08-07
BAOLINGBAO BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are a large number of domestic probiotics manufacturers, and the production process mostly uses solid fermentation. The production process is easy to contaminate miscellaneous bacteria, the content of bacteria is not easy to control, the output is unstable, and the degradation of production strains is serious. The actual effect in livestock and poultry breeding is not obvious. Caused the slow development of feed microecological agent industry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Preparation of Bacillus licheniformis inocula

[0024] (1) Primary seed culture: After activating Bacillus licheniformis on beef extract peptone slant medium for 16~24 h, transfer it to seed culture medium and shake flask culture for 16 h, as seed culture solution;

[0025] (2) Liquid fermentation multiplication culture: Transfer the above-mentioned seed culture liquid to the liquid fermentation medium at an inoculum of 7% of the volume of the fermentation medium. The culture temperature is 35°C. After 20 hours of culture, the effective viable cell count can reach 3.4×10 9 CFU / g;

[0026] (3) Centrifugal separation: Centrifuge the above fermentation broth at 4°C for 15 minutes, and remove the lower sediment for use;

[0027] (4) Add freeze-dried protective agent and vacuum freeze-drying: add 10% of the fermentation broth volume to the precipitate, and mix thoroughly, at a vacuum of 6 Pa, a freezing temperature of -40°C, and a freeze-drying time of 8 h. Obtained Bac...

Embodiment 2

[0028] Example 2: Preparation of Bacillus licheniformis inocula

[0029] (1) Primary seed culture: After activating Bacillus licheniformis on the beef extract peptone slant medium for 16~24 h, transfer it to the seed medium and shake it for 20 hours as the seed culture solution;

[0030] (2) Liquid fermentation multiplication culture: Transfer the above seed culture liquid to the liquid fermentation medium at an inoculum of 3% of the volume of the fermentation medium, the culture temperature is 35℃, and the effective viable cell count can reach after 24 hours of culture 5.6×10 9 CFU / mL;

[0031] (3) Centrifugal separation: Centrifuge the above fermentation broth at 4°C for 20 minutes, and remove the lower sediment for use;

[0032] (4) Add freeze-dried protective agent and vacuum freeze-drying: add 20% of the fermentation broth volume to the precipitate, and mix thoroughly, at a vacuum of 6 Pa, a freezing temperature of -40°C, and a freeze-drying time of 10 h. Obtained Bacillus lic...

Embodiment 3

[0033] Example 3: Application of Bacillus licheniformis inocula

[0034] Select 100 three-yuan (Duroc × Landrace × Dabai) hybrid piglets weaned at 30 days of age with an average weight of 7.20±0.30 kg. According to the average weight, they were randomly divided into 2 groups. One group only uses conventional feed formula without adding antibiotics and Invented inoculum product, another one adds 0.03% of the product of the present invention to conventional feed, carries out deworming, immunization and feed management according to conventional methods, and feeds continuously for 1 month. It is found that the inocula of Bacillus licheniformis of the present invention is added to conventional feed Compared with the other group, the diarrhea rate of one group was 62% lower, the protein digestibility increased by 6.3%, and the feed-to-meat ratio decreased by 3.4%.

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Abstract

The invention discloses a preparation method and an application of a feeding bacillus licheniformis (Baclicuslincheniformis) inoculant, and belongs to the field of feeder additives. The bacillus licheniformis BLBY-7 is selected from rumen of a cattle, which can secrete bacitracin, has a broad-spectrum antibacterial effect in intestinal tracts of livestock and poultry, can directly destroy pathogenic bacteria, leaves no toxin, and also can secrete high-activity protease, thus improving the digestibility and utilization rate of feed; and the inoculant contains more than 8-23*10<10>CFU / g of bacillus licheniformis, which is added to the general feed of livestock and poultry based on 0.01 to 0.05% by weight, and can effectively improve the resisting capacity of livestock and poultry to general diseases, as well as reduce the usage amount of antibiotics.

Description

technical field [0001] The invention relates to a feed probiotic agent, in particular to a feed additive whose effective microorganism is bacillus licheniformis. Background technique [0002] Feed antibiotics were considered to be the greatest biotechnology in animal husbandry production in the 20th century. Since it was first reported in 1946 that streptomycin can stimulate the growth of chicks, in 1950, the US Food and Drug Administration (FDA) approved antibiotics for the first time as feed After additives, antibiotics are not only widely used to treat and prevent bacterial diseases, but also widely promoted and applied as feed additives, which play an extremely important role in controlling infectious diseases of livestock and poultry and ensuring the healthy development of modern aquaculture. However, with the excessive abuse of antibiotics, its various drawbacks are gradually being recognized by people. At the same time, antibiotics are increasingly endangering people...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/16C12R1/10
Inventor 刘宗利王乃强胥九兵刘海玉李克文孔健施安辉季明杰李方华
Owner BAOLINGBAO BIOLOGY
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