Collagen/polyvinyl alcohol composite microspheres as well as preparation method and application thereof

A technology of collagen and polyvinyl alcohol, applied in the direction of animal/human protein, conjugated synthetic polymer artificial filament, cellulose/protein conjugated artificial filament, etc., can solve the problems that cannot meet the wide range of needs and applications of biomedical materials Single field and other problems, to achieve the effect of facilitating migration and proliferation, improving therapeutic effect, and good gel performance

Active Publication Date: 2013-09-04
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the products provided by the above-mentioned patents are all nanofibers, and their application fields are relatively single, which cannot meet the extensive needs of various biomedical materials.

Method used

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  • Collagen/polyvinyl alcohol composite microspheres as well as preparation method and application thereof
  • Collagen/polyvinyl alcohol composite microspheres as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Collagen / polyvinyl alcohol composite microspheres are composite systems composed of collagen / polyvinyl alcohol microspheres and collagen / polyvinyl alcohol nanofibers, such as figure 1 As shown, the composite system uses microspheres as nodes and is connected into a beaded or net shape by nanofibers, wherein the average diameter of the microspheres is 550 nm, and the average diameter of the nanofibers is 55 nm.

[0020] The preparation method of the collagen / polyvinyl alcohol composite microspheres comprises the following steps:

[0021] 1) Collagen is extracted from pigskin by pepsin-acid hydrolysis, and then small-molecular-weight collagen is removed by cellulose dialysis membrane with a molecular weight cut-off of 8,000-14,400. The remaining collagen has a high molecular weight and narrow distribution, and is formed after freeze-drying Porous collagen sponge; at 15°C, take 0.5g of collagen sponge and dissolve it in 50mL of 0.5M acetic acid aqueous solution, stir until...

Embodiment 2

[0026] Collagen / polyvinyl alcohol composite microspheres are a composite system composed of collagen / polyvinyl alcohol microspheres and collagen / polyvinyl alcohol nanofibers. The composite system uses microspheres as nodes and is connected by nanofibers to form beads shape or network, wherein the diameter of microspheres is 100-1000nm, and the diameter of nanofibers is 1-100nm.

[0027] The preparation method of the collagen / polyvinyl alcohol composite microspheres comprises the following steps:

[0028] 1) Collagen is extracted from pigskin by pepsin-acid hydrolysis, and then small-molecular-weight collagen is removed by cellulose dialysis membrane with a molecular weight cut-off of 8,000-14,400. The remaining collagen has a high molecular weight and narrow distribution, and is formed after freeze-drying Porous collagen sponge; at 20°C, take 0.75g of collagen sponge and dissolve it in 50mL of 0.8M acetic acid aqueous solution, stir until completely dissolved, and prepare a co...

Embodiment 3

[0031] Collagen / polyvinyl alcohol composite microspheres are a composite system composed of collagen / polyvinyl alcohol microspheres and collagen / polyvinyl alcohol nanofibers. The composite system uses microspheres as nodes and is connected by nanofibers to form beads shape or network, wherein the diameter of microspheres is 100-1000nm, and the diameter of nanofibers is 1-100nm.

[0032] The preparation method of the collagen / polyvinyl alcohol composite microspheres comprises the following steps:

[0033] 1) Collagen is extracted from pigskin by pepsin-acid hydrolysis, and then small-molecular-weight collagen is removed by cellulose dialysis membrane with a molecular weight cut-off of 8,000-14,400. The remaining collagen has a high molecular weight and narrow distribution, and is formed after freeze-drying Porous collagen sponge; at 30°C, take 0.35g of collagen sponge and dissolve it in 50mL of 0.5M acetic acid aqueous solution, stir until completely dissolved, and prepare a co...

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Abstract

The invention provides collagen/polyvinyl alcohol composite microspheres as well as a preparation method and application thereof, belonging to the field of high polymer composite materials. The microsphere diameter of the collagen/polyvinyl alcohol composite microspheres is 100-1000nm, and the microspheres are connected into the shape of a string of beads by using collagen/polyvinyl alcohol composite nano-fibers, or are connected into the shape of a net by taking the microspheres as nodes and using the composite nano-fibers. The preparation method of the collagen/polyvinyl alcohol composite microspheres comprises the following steps of: (1) dissolving collagen into an acetic acid aqueous solution to prepare a collagen solution of which the concentration is 7-25g/L; and (2) uniformly mixing the collagen solution with a polyvinyl alcohol aqueous solution of which the concentration is 6-12wt% in a weight ratio of (1-9):1, and performing electrostatic spinning, wherein the distance between a spinning needle head and a receiving plate is 8-30cm, the voltage is 8-30kV, the spinning flow rate is 0.1-10mL/h, and the spinning temperature is 25-35 DEG C. The collagen/polyvinyl alcohol composite microspheres provided by the invention can be used as a scaffold material for biological tissue engineering or used as a medicament slow-release material.

Description

technical field [0001] The invention belongs to the field of polymer materials, and in particular relates to a collagen / polyvinyl alcohol composite microsphere, and also relates to a preparation method and application of the composite microsphere. Background technique [0002] As the most direct and effective spinning method, electrospinning technology has been widely used in the preparation of polymer nanofibers. As a natural polymer material widely present in animal connective tissue, collagen has good biocompatibility, so it has broad application prospects in the field of biomedical materials. At present, although some patents using collagen for electrospinning have been disclosed in my country's national patent library, such as patent No. 200610116988.3 with the publication number CN1944724A, which provides a composite nanofiber of collagen and chitosan, but this patent is Using hexafluoroisopropanol and trifluoroacetic acid as solvents, a mixed solution of chitosan and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D01F8/02D01F8/10C07K14/78A61L27/26A61L27/50A61K47/42A61K47/32
Inventor 汤克勇张晓莉郑学晶王芳刘捷
Owner ZHENGZHOU UNIV
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