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Method for preparing diglyceride through goose fat enzymolysis

A technology of diglyceride and goose oil, which is applied in the field of enzymatic hydrolysis of goose oil to prepare diglyceride, which can solve the problems of goose oil waste and less goose oil, and achieve the effects of increasing yield, sufficient reaction, and shortening filtration time

Active Publication Date: 2013-09-11
QINGDAO RICHEN FOODS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is less research on goose oil at present, and most of the goose oil is used as feed and causes waste

Method used

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  • Method for preparing diglyceride through goose fat enzymolysis
  • Method for preparing diglyceride through goose fat enzymolysis
  • Method for preparing diglyceride through goose fat enzymolysis

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0030] 2) Preparation of mixed fatty acids: add the refined goose oil to 1mol / L NaOH-ethanol solution at a volume ratio of 1:4, stir magnetically for a period of time to completely dissolve it, then transfer it to a 1000ml three-necked flask, and reflux in a water bath under nitrogen protection Saponify for a period of time to obtain a saponified solution; distill ethanol out under reduced pressure, add water to completely dissolve the saponified product, acidify with 10% HCl to pH 2-3 under constant temperature and constant speed stirring, add a certain volume of n-hexane for extraction, and wash with warm water until Neutral, evaporate the solvent under reduced pressure, add anhydrous sodium sulfate for dehydration, and filter with suction to obtain mixed fatty acids. This not only makes the reaction more fully, but also shortens the reaction time to a certain extent, and improves the diglyceride formation rate. Among them, the preparation conditions of the mixed fatty acid ...

Embodiment 1

[0034] Example 1: Screening test for the optimum process conditions for the preparation of diglycerides from enzymatic hydrolysis of goose oil

[0035] 1. Refining of goose oil

[0036] The fat liver and goose belly fat is heated at 55°C to melt, and the impurities are removed by vacuum filtration to obtain crude goose oil; the crude goose oil is degummed, decolorized, and deodorized to obtain refined goose oil products.

[0037] 2. Preparation of mixed fatty acids

[0038] Add the refined goose oil to 1mol / L NaOH-ethanol solution in a ratio of 1:4, stir magnetically for a period of time to completely dissolve it, then transfer it to a 1000ml three-necked flask, and reflux it in a water bath at 82°C for 1 hour to obtain a saponified solution. In a rotary evaporator, evaporate ethanol under reduced pressure, add water to completely dissolve the saponified product, acidify with 10% HCl to pH 2-3 under constant temperature and constant speed stirring, separate layers, add a cert...

Embodiment 2

[0057] Example 2: Enzymatic hydrolysis of goose oil to prepare diglyceride optimal process conditions screening regression test one

[0058] Add glycerol and mixed fatty acids directly into the flask at a substrate ratio of 1:1.05, place on a constant shaker, and set the reaction temperature at 50°C. After the reactants are evenly mixed, add 2.25% mass fraction of Candida antarctica Lipase B, the reaction time is 9.2h. After the reaction is completed, centrifuge the enzymatic solution in a high-speed centrifuge for 10 minutes to separate the enzyme. The supernatant is a mixture of high diglycerides. generation rate. Through 5 verification tests, it was found that the production rate of diglyceride obtained under this condition was 92.05%, 92.37%, 92.28%, 92.25%, 92.34%, and the average extraction rate was 92.26%, which was basically consistent with the results of Example 1; The above results show that the process of the present invention has good stability.

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Abstract

The invention provides a method for preparing diglyceride through goose fat enzymolysis. According to the invention, fat is hydrolyzed with a chemical saponification method, such that diglyceride is prepared. According to the invention, during the diglyceride preparation process, a goose fat refining step and a vacuum pump filtration step are added, such that impurities can be removed, filtration time can be shortened, goose fat purity and extraction rate can be improved, and further diglyceride yield can be improved. Also, when the goose fat diglyceride is prepared with the enzymatic method, the process is carried out on the basis of fat hydrolysis, such that reaction is more sufficient, reaction time is shortened to a certain extent, and diglyceride yield can be improved. With the method provided by the invention, a blank in the field is filled, a novel method is provided for the preparation of goose fat diglyceride, and important economic and social value are provided.

Description

technical field [0001] The invention belongs to the technical field of diglyceride preparation, and in particular relates to a method for enzymolyzing goose oil to prepare diglyceride. Background technique [0002] Diacylglycerol (DG) is a structural lipid in which a fatty acid in a class of triacylglycerol (TG) is replaced by a hydroxyl group. It is a trace component of natural vegetable oils and an endogenous intermediate product of fat metabolism in the body. It is recognized as Safe (GRAS certified) food ingredients. Studies have found that the digestion and metabolism pathways of diacylglycerols and triglycerides are significantly different. Most of the diglycerides are decomposed into 1-MG and fatty acids that can no longer be synthesized into fat. Because the position of the combination of fatty acids and glycerol in 1-MG and 2-MG is different, there is a big difference as a raw material for neutral fat synthesis. In the small intestine The resynthesis of inward neu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
Inventor 王宝维韩海娜葛文华张名爱岳斌
Owner QINGDAO RICHEN FOODS
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