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Method for preparing grapheme-titanium dioxide composite electrochemical biosensor

A biosensor, titanium dioxide technology, applied in the direction of electrochemical variables of materials, scientific instruments, instruments, etc., can solve the problems of limited application and poor conductivity of electrochemical biosensors, and achieve simple process methods, low detection limits, and biological affinity. good sex effect

Inactive Publication Date: 2013-09-18
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the invention is to provide a preparation method of graphene-titanium dioxide composite electrochemical biosensor, which solves the problem of existing TiO2 2 Poor conductivity of electrochemical biosensors leads to limited applications

Method used

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  • Method for preparing grapheme-titanium dioxide composite electrochemical biosensor
  • Method for preparing grapheme-titanium dioxide composite electrochemical biosensor
  • Method for preparing grapheme-titanium dioxide composite electrochemical biosensor

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Experimental program
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Effect test

Embodiment 1

[0043] Graphene oxide was prepared by the improved Hummers method. Take 1 g of pre-prepared graphite oxide, disperse it in 40 mL of deionized water, and ultrasonicate for 3 h. After centrifugation, the supernatant was taken to obtain graphene oxide. Take 200-400mL of absolute ethanol, add 0.5-2.0mL of potassium chloride solution with a concentration of 0.4mmol / L, stir for 10-30min, add a certain amount of graphene oxide powder, stir for a certain period of time, slowly drop in 3-6mL of Butyl titanate was stirred for 10 minutes, aged for 3-6 hours, washed by centrifugation, dried, and placed in an argon atmosphere furnace. The heating rate is 2°C / min before 300°C. When between 300°C and 500°C, the heating rate is 1°C / min. Insulate at 500°C for 2h. A graphene-supported titanium dioxide composite material was obtained.

[0044] Polish the glassy carbon electrode with 1.0, 0.3, and 0.05 μm alumina powder in turn, then ultrasonically clean the electrode surface with deionized w...

Embodiment 2

[0048] Graphene oxide was prepared by the improved Hummers method. Take 1 g of pre-prepared graphite oxide, disperse it in 40 mL of deionized water, and ultrasonicate for 3 h. After centrifugation, the supernatant was taken to obtain graphene oxide. Take 200-400mL of absolute ethanol, add 0.5-2.0mL of sodium chloride solution with a concentration of 1mmol / L, stir for 10-30min, add a certain amount of graphene oxide powder, stir for a certain period of time, slowly drop in 3-6mL of titanium Acetyl butyl ester, stirred for 10min. Aging 3-6h. Washed by centrifugation, dried, and placed in an argon atmosphere furnace. The heating rate is 2°C / min before 300°C. When between 300°C and 500°C, the heating rate is 1°C / min. Insulate at 500°C for 2h. A graphene-supported titanium dioxide composite material was obtained.

[0049] Polish the glassy carbon electrode with 1.0, 0.3, and 0.05 μm alumina powder in turn, then ultrasonically clean the electrode surface with deionized water f...

Embodiment 3

[0052] Graphene oxide was prepared by the improved Hummers method. Take 1 g of pre-prepared graphite oxide, disperse it in 40 mL of deionized water, and ultrasonicate for 3 h. After centrifugation, the supernatant was taken to obtain graphene oxide (GO). Take 200-400mL of absolute ethanol, add 0.5-2.0mL of potassium nitrate solution with a concentration of 0.8mmol / L, stir for 10-30min, add a certain amount of graphene oxide powder, stir for a certain period of time, slowly drop in 3-6mL of titanium Acetyl butyl ester, stirred for 10min. Aging 3-6h. Washed by centrifugation, dried, and placed in an argon atmosphere furnace. The heating rate is 2°C / min before 300°C. When between 300°C and 500°C, the heating rate is 1°C / min. Insulate at 500°C for 2h. Graphene-supported titanium dioxide composites (GO-TiO 2 ).

[0053] Polish the glassy carbon electrode with 1.0, 0.3, and 0.05 μm alumina powder in turn, then ultrasonically clean the electrode surface with deionized water fo...

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Abstract

The invention discloses a method for preparing a grapheme-titanium dioxide composite electrochemical biosensor. The preparation method is orderly carried out according to the following steps of: preparing a grapheme oxide; preparing a graphene load titanium dioxide composite material; preparing a glassy carbon electrode; preparing a decorative glassy carbon electrode. The method for preparing the grapheme-titanium dioxide composite electrochemical biosensor is simple in technique, available in material and free of pollution. The product prepared by the preparation method achieves direct electron transfer of horseradish peroxidase and electrodes, and has the advantages of low detection limit, short response time, good biocompatibility, high sensitivity, good stability and the like.

Description

technical field [0001] The invention belongs to the field of material preparation and electrochemical technology, and relates to a preparation method of an electrochemical biosensor, in particular to a preparation method of a graphene-titania composite electrochemical biosensor. Background technique [0002] Horseradish catalase is a typical oxidoreductase. Horseradish peroxidase (HRP) has the characteristics of high activity, good stability, small molecular weight and easy preparation, so it is most commonly used in sensor electrodes. Its physiological function is to catalyze H 2 o 2 reduction and H 2 o 2 The oxidation of many substrates that are intermediate products can be immobilized on the electrode surface by methods such as adsorption, covalent bonding, sol-gel method, and polymer embedding. [0003] TiO 2 Nanomaterials have the characteristics of excellent biocompatibility, high electrical conductivity and low cost, and TiO 2 The application of nanomaterials as...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/327
Inventor 刘辉苏兴段聪越董晓楠李军奇朱振峰
Owner SHAANXI UNIV OF SCI & TECH
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