Method for preparing grapheme-titanium dioxide composite electrochemical biosensor

A biosensor, titanium dioxide technology, applied in the direction of electrochemical variables of materials, scientific instruments, instruments, etc., can solve the problems of limited application and poor conductivity of electrochemical biosensors, and achieve simple process methods, low detection limits, and biological affinity. good sex effect

Inactive Publication Date: 2013-09-18
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the invention is to provide a preparation method of graphene-titanium dioxide composite electrochemical bio

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  • Method for preparing grapheme-titanium dioxide composite electrochemical biosensor
  • Method for preparing grapheme-titanium dioxide composite electrochemical biosensor
  • Method for preparing grapheme-titanium dioxide composite electrochemical biosensor

Examples

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Example Embodiment

[0042] Example 1

[0043] Graphene oxide was prepared by the improved Hummers method. 1 g of pre-prepared graphite oxide was taken, dispersed in 40 mL of deionized water, and sonicated for 3 hours. After centrifugation, the supernatant was taken to obtain graphene oxide. Take 200-400mL absolute ethanol, add 0.5-2.0mL potassium chloride solution with a concentration of 0.4mmol / L, stir for 10-30min, add quantitative graphene oxide powder, stir for a certain period of time, slowly drip 3-6mL Stir butyl titanate for 10 minutes, age for 3-6 hours, centrifuge, wash, dry, and place it in an argon atmosphere furnace. The heating rate before 300°C is 2°C / min. When between 300℃-500℃, the heating rate is 1℃ / min. Incubate at 500°C for 2h. The graphene-supported titanium dioxide composite material is obtained.

[0044] The glassy carbon electrode was polished with 1.0, 0.3, and 0.05μm alumina powder in sequence, and then the electrode surface was ultrasonically cleaned with deionized water...

Example Embodiment

[0047] Example 2

[0048] Graphene oxide was prepared by the improved Hummers method. 1 g of pre-prepared graphite oxide was taken, dispersed in 40 mL of deionized water, and sonicated for 3 hours. After centrifugation, the supernatant was taken to obtain graphene oxide. Take 200-400mL of absolute ethanol, add 0.5-2.0mL of sodium chloride solution with a concentration of 1mmol / L, stir for 10-30min, add quantitative graphene oxide powder, stir for a certain period of time, slowly drop 3-6mL of titanium Butyl acid, stirring for 10min. Aging for 3-6h. Centrifugal washing, drying, and placing it in an argon atmosphere furnace. The heating rate before 300°C is 2°C / min. When between 300℃-500℃, the heating rate is 1℃ / min. Incubate at 500°C for 2h. The graphene-supported titanium dioxide composite material is obtained.

[0049] The glassy carbon electrode was polished with 1.0, 0.3, and 0.05μm alumina powder in sequence, and then the electrode surface was ultrasonically cleaned with...

Example Embodiment

[0051] Example 3

[0052] Graphene oxide was prepared by the improved Hummers method. 1 g of pre-prepared graphite oxide was taken, dispersed in 40 mL of deionized water, and sonicated for 3 hours. After centrifugation, the supernatant is taken to obtain graphene oxide (GO). Take 200-400mL of absolute ethanol, add 0.5-2.0mL of 0.8mmol / L potassium nitrate solution, stir for 10-30min, add quantitative graphene oxide powder, stir for a certain period of time, slowly drip 3-6mL of titanium Butyl acid, stirring for 10min. Aging for 3-6h. Centrifugal washing, drying, and placing it in an argon atmosphere furnace. The heating rate before 300°C is 2°C / min. When between 300℃-500℃, the heating rate is 1℃ / min. Incubate at 500°C for 2h. The graphene-supported titanium dioxide composite material (GO-TiO 2 ).

[0053] The glassy carbon electrode was polished with 1.0, 0.3, and 0.05μm alumina powder in sequence, and then the electrode surface was ultrasonically cleaned with deionized water...

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Abstract

The invention discloses a method for preparing a grapheme-titanium dioxide composite electrochemical biosensor. The preparation method is orderly carried out according to the following steps of: preparing a grapheme oxide; preparing a graphene load titanium dioxide composite material; preparing a glassy carbon electrode; preparing a decorative glassy carbon electrode. The method for preparing the grapheme-titanium dioxide composite electrochemical biosensor is simple in technique, available in material and free of pollution. The product prepared by the preparation method achieves direct electron transfer of horseradish peroxidase and electrodes, and has the advantages of low detection limit, short response time, good biocompatibility, high sensitivity, good stability and the like.

Description

technical field [0001] The invention belongs to the field of material preparation and electrochemical technology, and relates to a preparation method of an electrochemical biosensor, in particular to a preparation method of a graphene-titania composite electrochemical biosensor. Background technique [0002] Horseradish catalase is a typical oxidoreductase. Horseradish peroxidase (HRP) has the characteristics of high activity, good stability, small molecular weight and easy preparation, so it is most commonly used in sensor electrodes. Its physiological function is to catalyze H 2 o 2 reduction and H 2 o 2 The oxidation of many substrates that are intermediate products can be immobilized on the electrode surface by methods such as adsorption, covalent bonding, sol-gel method, and polymer embedding. [0003] TiO 2 Nanomaterials have the characteristics of excellent biocompatibility, high electrical conductivity and low cost, and TiO 2 The application of nanomaterials as...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/327
Inventor 刘辉苏兴段聪越董晓楠李军奇朱振峰
Owner SHAANXI UNIV OF SCI & TECH
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