Egg yolk composite feed additive and preparation method thereof
A feed additive and compound feed technology, applied in the direction of animal feed, animal feed, application, etc., can solve the problems of affecting the activity of egg yolk antibody, the loss of egg yolk antibody activity, and the high temperature of spray drying, so as to increase the benefits of industrialization and improve digestion. function, the effect of lowering intestinal pH
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Embodiment 1
[0043] The preparation of embodiment 1 immune egg
[0044] 1) Prepare mixed antigen:
[0045] Five species of Escherichia coli (K88\K99\987P\F18\F41) that cause diarrhea in piglets were cultured on a large scale under appropriate medium (TSA, Slanetz and Minca agar medium) and culture conditions, and the bacterial liquid was collected and used The pilus and the thallus are broken and separated by the shaking method in a water bath, and the pilus liquid without the bacterium is obtained after two times of centrifugation. The pili liquid was mixed according to the concentration ratio of 1:1:1:1:1 to obtain the mixed antigen, the final concentration of the mixed antigen was 50-500 mg / ml, and stored at -80°C for later use.
[0046]It should be noted that: in the present invention, any one or a combination of at least two of the five Escherichia coli that can cause diarrhea in piglets can be used as an antigen to immunize laying poultry. However, in order to obtain a better bacte...
Embodiment 2
[0051] The preparation of embodiment 2 egg yolk liquid
[0052] 1) Sampling and storage of eggs. Raw eggs go through strict sensory inspection, physical and chemical inspection and microbiological inspection according to fresh egg hygienic standard GB2748-2003, especially pesticide residues, drug residues, heavy metals and pathogenic bacteria. The storage conditions are 4-15 degrees Celsius temperature conditions and about 80% relative humidity. Place it in a fixed clean storage area to store enough supply for 7 days egg production.
[0053] 2) Cleaning and disinfection of the upper (loading) immune eggs and immune eggs. The operator places the egg trays on the rotary table. The egg loader loads the eggs onto the rolling table through the vacuum nozzle. The egg loader has a special jam detection system that transfers empty egg trays to the operator. After the eggs are loaded onto the rolling table, the automatic blowback system can ensure the next round of work of the vac...
Embodiment 3
[0055] The preparation of embodiment 3 egg yolk particles
[0056] 1) Ingredients. On the basis of calculating the volume of the egg yolk liquid, weigh the microcrystalline cellulose and xylooligosaccharide according to the proportion, and add the microcrystalline cellulose and xylooligosaccharide to the In the homogenate tank containing egg yolk liquid, increase the stirring speed after all the two auxiliary materials are added to the homogenate filling, so that the three components are fully mixed.
[0057] 2) Extrude. Add the mixture into the spheronizer in batches, slowly add the mixture prepared in step 1 at a hole diameter of 4.5 mm and a rotation speed of 50 r / min, and extrude a long continuous strip solid semi-finished product with a smooth surface at the outlet of the extruder.
[0058] 3) Roll round. Collect the semi-finished products and add them to the spheronizer for spheronization. The time is about 1min. The processing speed is initially 200r / min, and then qu...
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