Supercharge Your Innovation With Domain-Expert AI Agents!

Method for promoting collard stubborn genotype microspore embryogenesis

A technology of kale and embryogenesis, which is applied in the field of plant tissue culture, can solve the problems of difficult identification and long breeding time, and achieve the effects of quality improvement, workload reduction and operability

Inactive Publication Date: 2013-11-20
ZHENJIANG SUIHAN AGRI
View PDF2 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method helps to promote the emergence of kale, because it is co-cultivated with rapeseed microspores, the embryoid body obtained is a mixed embryoid body of kale and rapeseed, which is difficult to identify and can only be judged after planting in the field. Breeding time is longer

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Culture medium preparation: culture medium including microspores in different culture stages, its components and the weight of each component in each liter of medium are:

[0027] ①NLN-13 liquid medium: NLN liquid medium 1L + sucrose 130g, pH6.0, filter sterilized;

[0028] NLN liquid medium, in 1L, consists of: KNO 3 125mg, Ca(NO 3 ) 2 4H 2 O 500mg, MgSO 4 ·7H 2 O 125mg, KH 2 PO 4 125 mg, H 3 BO 3 6.2 mg, MnSO 4 ·H 2 O 18.95mg, ZnSO 4 ·7H 2 O 8.6 mg, Na 2 MoO 4 2H 2 O 0.25mg, CuSO 4 ·5H 2 O 0.025mg, CoCl 2 ·6H 2 O 0.025mg, vitamin B1 0.5mg, vitamin B6 0.5mg, biotin 0.05mg, folic acid 0.5mg, Na 2 EDTA 37.3mg, FeSO 4 ·7H 2 O 27.8mg, inositol 100mg, glycine 2mg, niacin 5mg, L-glutamine 800mg, glutathione 30mg, vitamin B5 5mg, serine 100mg and the rest sterile water.

[0029] ② Embryoid body differentiation medium: B5 medium 1L + sucrose 30g + agar 9g, pH 6.0, high temperature and high pressure sterilization;

[0030] B5 medium, in 1L, consis...

Embodiment 2

[0045] (1) Culture medium preparation

[0046] ① NLN-13 liquid medium: NLN medium 1L + sucrose 130g, pH 6.2, filter sterilized; ② Embryoid differentiation medium: B5 medium 1L + sucrose 30g + agar 10g, pH 6.1, high temperature and high pressure sterilization; ③ Rooting Medium: MS medium 1L + white sugar 20g + agar 7g, pH 6.0, high temperature and high pressure sterilization;

[0047] (2) Method for promoting microspore embryogenesis of kale obstinate genotype:

[0048] ① Flower bud selection: take kale (the ratio of petals to anthers on the inflorescence is 1.0) and rapeseed (the ratio of petals to anthers is 0.6) in late uninucleate to early binucleate, healthy, and free of diseases and insect pests, as the supply for microspore culture. body;

[0049] ②Sterilization of flower buds: Put rapeseed and kale flower buds into sterile petri dishes, add 0.1% (mass percentage concentration) mercury chloride solution, seal and put on a shaker to shake the surface for 8 minutes, and ...

Embodiment 3

[0056] (1) Culture medium preparation

[0057] ① NLN-13 liquid medium: NLN-13 medium 1L + sucrose 130g, pH 6.1, filter sterilized; ② Embryoid differentiation medium: B5 medium 1L + sucrose 30g + agar 10g, pH 6.0, high temperature and high pressure sterilization; ③ Rooting medium: MS medium 1L + white sugar 20g + agar 7.5g, pH5.9, high temperature and high pressure sterilization;

[0058] (2) Method for improving microspore embryogenesis of kale obstinate genotype:

[0059] ① Flower bud selection: Take kale (the ratio of petals to anthers on the inflorescence is 1.1) and rapeseed (the ratio of petals to anthers is 0.75) in late uninucleate to early binucleate, healthy, and free of diseases and insect pests, as the supply for microspore culture. body;

[0060] ②Sterilization of flower buds: Put the rapeseed and kale flower buds into sterile petri dishes respectively, add 0.1% (mass percentage concentration) mercury chloride solution, seal it, put it on a shaker and shake the s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for promoting collard stubborn genotype microspore embryogenesis. The method comprises carrying out co-culture of a sterile oilseed rape anther and collard microspore mixed suspension liquid to obtain an embryo, wherein in an initial stage, it is determined that the embryo is a collard embryo. The collard microspores are in a free state and oilseed rape microspores are in sterile oilseed rape anthers and thus differentiation and identification of an oilseed rape plant in a microspore plant colony are avoided in regenerated plant transplantation so that labor is reduced and collard stubborn genotype microspore embryogenesis efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a culture method for promoting microspore embryogenesis of kale obstinate genotype. Background technique [0002] Kale (Brassica oleracea L. var. acephala), also known as leaf peony, is a variety of Brassicaceae Brassica species, a biennial herb, native to the Mediterranean and Asia Minor, and is the closest wild species of cabbage vegetables and foliage plants. Nowadays, it is planted more in the United Kingdom, the Netherlands, Germany and the United States, and there are ornamental varieties, vegetable varieties, and both ornamental and vegetable varieties. The history of introduction and cultivation in our country is not long, especially vegetable kale has only been planted in a small amount in the past ten years, mainly distributed in large and medium-sized cities such as Beijing, Shanghai, and Guangzhou. Ornamental varieties are also known as colored cabbage,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H4/00
Inventor 张振超戴忠良潘跃平毛忠良姚悦梅秦文斌潘永飞肖燕吴国平孙春青马志虎
Owner ZHENJIANG SUIHAN AGRI
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com