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Test paper for colloidal gold immunochromatograohic assay of IgG (immunoglobulin G) antibody of dog anti-rabies virus and preparation method of test paper

A technology for immunochromatographic detection and rabies virus, which is applied in the direction of measuring devices, analysis materials, instruments, etc., can solve problems such as the inability to determine the level of antibodies, and achieve rapid detection, high detection accuracy, and good detection repeatability.

Active Publication Date: 2013-11-20
WUHAN CHOPPER BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing patents on rabies virus antibody colloidal gold test strips, there is generally one control line (or quality control line), and the result interpretation is based on the color depth of the test line, and the antibody level cannot be judged

Method used

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  • Test paper for colloidal gold immunochromatograohic assay of IgG (immunoglobulin G) antibody of dog anti-rabies virus and preparation method of test paper
  • Test paper for colloidal gold immunochromatograohic assay of IgG (immunoglobulin G) antibody of dog anti-rabies virus and preparation method of test paper
  • Test paper for colloidal gold immunochromatograohic assay of IgG (immunoglobulin G) antibody of dog anti-rabies virus and preparation method of test paper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Canine anti-rabies virus IgG antibody colloidal gold immunochromatography test paper planar structure area diagram figure 1 As shown, the horizontal plane of the test paper from bottom to top is: sample absorption area 1, gold label probe area 2, immobilized antibody area 3 and water absorption area 4, immobilized antibody area 3 is coated with control line C1 31, detection Line T 32, control line C2 33 and control line C3 34.

[0034] Structural diagram of the longitudinal section of the colloidal gold immunochromatographic detection test paper for dog anti-rabies virus IgG antibody (specific materials are used to represent each area), the polyethylene plate 5 with a layer of polyvinyl chloride lining film is the support plate, and the glass fiber film 6 is the sample absorption The polyester film 7 is the gold standard probe area, the nitrocellulose membrane 8 is the solid-phase antibody area, and the water-absorbing filter paper 9 is the water-absorbing area. The pol...

Embodiment 2

[0048] Except that the coating amount of the detection line T is 5 μg; the coating amount of the control line C1 is 0.5 μg, the coating amount of the control line C2 is 5 μg, and the coating amount of the control line C3 is 2 μg, and the coating amount of the rabies virus antigen purified by the glass fiber membrane is 20 μg; the labeling amount of gold-labeled probe 1 rabies virus monoclonal antibody is 20 μg monoclonal antibody per mL of colloidal gold, and the labeling amount of gold-labeled probe 2 rabbit IgG is 10 μg per mL of colloidal gold; colloidal gold-labeled rabies virus monoclonal Antibody method: Take 20mL of colloidal gold with a radius of 10nm and a concentration of 0.01% and 400μg of rabies virus monoclonal antibody, combine them by magnetic stirring and shaking under the condition of pH 9.0, add bovine serum albumin (BSA) and poly Ethylene glycol 20000 (PEG20000) was used as a stabilizer, and the final mass concentration of BSA was 2%, and the final mass conce...

Embodiment 3

[0050] Except that the coating amount of the detection line T is 2 μg; the coating amount of the control line C1 is 0.2 μg, the coating amount of the control line C2 is 2 μg, and the coating amount of the control line C3 is 5 μg, and the coating amount of the rabies virus antigen purified by glass fiber membrane is 50 μg; labeling amount of rabies virus monoclonal antibody for gold-labeled probe 1 is 10 μg of monoclonal antibody per mL of colloidal gold; labeling amount of gold-labeled probe 2 rabbit IgG is 5 μg of rabbit IgG per mL of colloidal gold; colloidal gold-labeled rabies virus monoclonal Antibody method: take 20mL of 0.01% colloidal gold with a radius of 20nm and 200μg of rabies virus monoclonal antibody, combine them by magnetic stirring and shaking under the condition of pH9.2, add bovine serum albumin (BSA) and poly Ethylene glycol 20000 (PEG20000) was used as a stabilizer, and the final mass concentration of BSA was 5%, and the final mass concentration of PEG20000...

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Abstract

The invention discloses a piece of test paper for colloidal gold immunochromatograohic assay of an IgG (immunoglobulin G) antibody of dog anti-rabies virus and a preparation method of the test paper. The test paper comprises a sample absorbing region, a gold-labeled probe region, a solid-phase antibody region, a water absorbing region and a supporting plate, wherein the sample absorbing region, the gold-labeled probe region, the solid-phase antibody region and the water absorbing region are laid on the supporting plate and are partially overlapped in sequence; the sample absorbing region is coated with rabies virus antigen; the gold-labeled probe region is coated with a gold-labeled probe 1 and a gold-labeled probe 2 which are a monoclonal antibody of the colloidal gold-labeled rabies virus and rabbit IgG respectively; the solid-phase antibody region has a control line C1 (coated with a goat anti-rabbit IgG antibody), a testing line T (coated with a goat anti-dog IgG antibody), a control line C2 (coated with a goat anti-rabbit IgG antibody) and a control line C3 (coated with a goat anti-mouse IgG antibody). The test paper disclosed by the invention has the advantages that the test is fast, the accuracy rate is high, the specificity is strong, the test paper is simple and convenient to carry and operate, and the level of the rabies virus antibody is judged according to the color depths of the control lines.

Description

technical field [0001] The invention relates to a detection test paper and a preparation method, in particular to a colloidal gold immunochromatographic detection test paper for dog anti-rabies virus IgG antibody and a preparation method. Background technique [0002] Rabies is a zoonotic infectious disease caused by Rabies virus (RV), and its main hosts are dogs, cats and other animals. The typical symptom is hydrophobia, also known as: hydrophobia. The disease is extremely dangerous, and the case fatality rate is almost 100%. my country is a country with a high incidence of rabies, ranking second in the world, second only to India. There are an average of more than 1,500 cases in my country every year, about half of which occur in Guangxi, Hunan, and Guizhou provinces, and the RV rate in dogs in these three provinces is as high as 2.3%. The brain rate of healthy dogs is about 5.88-13.13%, which poses a great threat to human health. With the development of the economy a...

Claims

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Application Information

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IPC IPC(8): G01N33/577
Inventor 廖园园漆世华秦伟刘洁李建谢红玲温文生朱薇冯钊
Owner WUHAN CHOPPER BIOLOGY
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