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Adsorbent for removing endotoxin by blood perfusion and preparation method thereof

A blood perfusion and adsorbent technology, applied in the field of biomedicine, can solve the problems of reducing the biological activity of ligands, complicated preparation process, leakage of ligands, etc., and achieves the effects of good safety, simple preparation method and lower production cost.

Inactive Publication Date: 2013-11-27
TIANJIN YOUNASI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This adsorbent uses molecular clusters to be fixed first and then small molecules are grafted on the molecular clusters as ligands to increase the content of ligands. However, the molecular clusters are large molecules, and the amount that can be fixed on the carrier itself is very low, and the preparation process is complicated and expensive. expensive
[0008] Polymyxin B is a more specific affinity ligand for endotoxin, and has been studied and applied more. However, polymyxin B has potential renal and neurotoxicity, and the way of immobilization on the carrier should be considered. Will reduce the biological activity of the ligand, non-covalent linkage has the risk of ligand leakage

Method used

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  • Adsorbent for removing endotoxin by blood perfusion and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Preparation of carrier materials

[0041] Add 10g of vinyl acetate, 20g of triallyl isocyanurate, 3ml of ethyl acetate and 3ml of n-heptane into a 500ml three-necked flask, heat up to 40°C, stir well and add 0.3g of azobisisobutyronitrile After dissolving, add 1000ml of aqueous solution (containing 1.5% polyvinyl alcohol and 3% sodium chloride), adjust the stirring speed to disperse the droplets evenly, raise the temperature to 65°C, heat-preserve and polymerize for 1h, then raise the temperature to 75°C and maintain for 5h, filter A white spherical copolymer was obtained, washed with water, fully washed with ethanol, and dried in the air. The above-mentioned copolymer microspheres were added to a solution composed of 12g NaOH and 500ml methanol, and the transesterification reaction (or alcoholysis reaction) was carried out at 40°C for 18h. After the reaction was completed, it was filtered with suction, washed with methanol, and dried in the air to obtain the carrie...

Embodiment 2

[0048] (1) Preparation of carrier materials

[0049] Add 20g of vinyl acetate, 10g of triallyl isocyanurate, 15ml of ethyl acetate and 15ml of n-heptane into a 500ml three-necked flask, heat up to 40°C, stir well and add 0.3g of azobisisobutyronitrile After dissolving, add 450ml of aqueous solution (containing 1.5% polyvinyl alcohol and 3% sodium chloride), adjust the stirring speed to disperse the droplets evenly, raise the temperature to 65°C, keep warm for 2h, then raise the temperature to 75°C for another 3h, filter A white spherical copolymer was obtained, washed with water, fully washed with ethanol, and dried in the air. The above-mentioned copolymer microspheres were added to a solution composed of 12g NaOH and 500ml methanol, and the transesterification reaction (or alcoholysis reaction) was carried out at 40°C for 18h. After the reaction was completed, it was filtered with suction, washed with methanol, and dried in the air to obtain the carrier. The hydroxyl conten...

Embodiment 3

[0055] (1) Preparation of carrier materials

[0056] Add 24g of vinyl acetate, 6g of triallyl isocyanurate, 60ml of ethyl acetate and 60ml of n-heptane into a 500ml three-necked flask, heat up to 40°C, stir well and add 0.3g of azobisisobutyronitrile After dissolving, add 750ml of aqueous solution (containing 1.5% polyvinyl alcohol and 3% sodium chloride), adjust the stirring speed to disperse the droplets evenly, raise the temperature to 65°C, keep warm for 5h, then raise the temperature to 75°C and maintain for 1h, filter A white spherical copolymer was obtained, washed with water, fully washed with ethanol, and dried in the air. The above-mentioned copolymer microspheres were added to a solution composed of 12g NaOH and 500ml methanol, and the transesterification reaction (or alcoholysis reaction) was carried out at 40°C for 18h. After the reaction was completed, it was filtered with suction, washed with methanol, and dried in the air to obtain the carrier. The hydroxyl co...

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Abstract

The invention relates to an adsorbent for removing endotoxin by blood perfusion and a preparation method thereof. The adsorbent uses a porous spherical synthesized high molecular material polyvinyl alcohol-triallyl isocyanurate copolymer as a carrier which has a rigid structure, great aperture and hydrophilic surfaces. The ligand is aliphatic diamine, and the carrier activated is coupled with the ligand by way of covalent bonds. The adsorbent is simple to prepare, good in stability, high in content of ligands and good in blood compatibility. The adsorbent is used by way of plasma or whole blood perfusion in blood purification to remove endotoxin in excessive content in blood of patients.

Description

technical field [0001] The invention belongs to the technical field of biomedicine. It relates to an adsorbent for hemoperfusion to remove endotoxin and a preparation method thereof. Background technique [0002] Endotoxin (ET), a highly toxic inflammatory and pyrogenic substance, is a lipopolysaccharide (LPS). The chemical structure of endotoxin is relatively complex and essentially consists of three parts: polysaccharide O-specific antigen, core polysaccharide and lipid A, in which lipid A is composed of glucosamine phosphate disaccharides and long-chain fatty acids, and is considered to be an endotoxin The biologically active center of the toxin. [0003] Endotoxin is released from the cell wall of Gram-negative bacteria when it dies, and enters the blood to form endotoxemia, which can be seen in various clinical processes, such as severe trauma, large area burns, severe inflammation, etc., and has a high lethality (40%-90%), if not treated in time, it will further le...

Claims

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Application Information

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IPC IPC(8): B01J20/26B01J20/28B01J20/30
Inventor 欧来良王为超俞耀庭
Owner TIANJIN YOUNASI BIOTECH
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