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Ovomucin liquid preparation and preparation method thereof

A technology of ovomucin and liquid preparations, applied in the field of ovomucin preparations and its preparation, can solve the problems of low solubility, inaccessibility, loss of antibacterial and antiviral activity, etc., achieve strong resistance activity, resist microbial invasion, and reduce virus The effect of activity and virulence

Active Publication Date: 2013-12-11
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, no ovomucin product has been able to reach the level of commercial application. Two important reasons are that the antibacterial and antiviral activities of ovomucin will be lost after purification and preparation, and its low solubility Cannot reach the concentration required for its activity

Method used

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  • Ovomucin liquid preparation and preparation method thereof
  • Ovomucin liquid preparation and preparation method thereof
  • Ovomucin liquid preparation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Effect of auxiliary protein on the adhesion effect of ovomucin bacteria

[0027] Studies have shown that ovomucin can limit the activity of microorganisms through adhesion, and then inhibit the growth, proliferation and toxicity of microorganisms. In the egg white of poultry eggs, in the natural state, other egg white proteins such as ovomucin and lysozyme form a thick protein in the form of aggregation, which provides a barrier for poultry eggs to resist microbial invasion. Therefore, the present invention simulates the natural state to study the influence of different auxiliary proteins on the adhesion ability of ovomucin bacteria.

[0028] Add different types and proportions of auxiliary proteins to high-purity ovomucin according to the proportions in Table 1 to make a protein mixture, and then use Escherichia coli, Salmonella and Staphylococcus aureus as objects, and use enzyme-linked immunosorbent assay (ELISA ) to determine the adhesion effect of differ...

Embodiment 2

[0035] Example 2 The effect of salt on the antiviral activity of ovomucin

[0036] Dissolve ovomucin in buffer solutions containing different salts (150mmol / L), stir well and centrifuge, take the precipitate and dissolve it again with the above-mentioned salt solution, adjust the final protein concentration to 50μg / mL, and use SDS-PAGE electrophoresis method to determine Sample purity. At the same time, refer to the national standard GB / T 14926.53-2001 and GB / T14926.54-2001 to determine the hemagglutination and hemagglutination inhibitory (HI) activity of ovomucin against chicken Newcastle disease virus, and use the half inhibitory concentration (IC50) of hemagglutination titer As an indicator of antiviral activity, the effects of different types of salt treatments on the purity and antiviral activity of ovomucin were studied, and the results are shown in Table 2.

[0037] Table 2 Effects of salt types on the antiviral effect of ovomucin preparations

[0038] salt t...

Embodiment 3

[0044] Example 3 Solubility Test of Ovomucin

[0045] Ovomucin is highly insoluble, and its saturation concentration in common buffers is about 0.1 mg / mL. In order to give full play to the role of ovomucin, its solubility needs to be increased. Amino acids are small molecular organic substances, which can not only increase the ionic strength in solution, but also interact with proteins to change the surface charge of proteins, thereby increasing the solubility of proteins. Therefore, the present invention investigated the effect of several amino acids on the solubility of ovomucin.

[0046] Add 1g of ovomucin to 100mL of buffer solution containing different kinds of amino acids (100mmol / L), mix evenly after constant volume, and stir for 6-8h. Centrifuge at 1500r / min for 5min, and use the Kjeldahl method to measure the nitrogen content in the added total protein, supernatant and the corresponding solvent respectively. The solubility of the solvent is expressed by nitrogen solu...

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Abstract

The invention discloses an ovomucin liquid preparation. The ovomucin liquid preparation comprises the following components by weight percent: 0.01-0.1% of ovomucin, 0.001-0.01% of accessory protein, 1.5-4% of amino acid, 0.5-5% of polyhydric alcohols, and the balance of buffer solution of which the pH value is 6-8 or water, wherein the accessory protein is lysozyme and / or ovotransferrin; and 1L of the ovomucin liquid preparation also contains 50-500mmol of calcium ion or 10-500 mmol of magnesium ion. The ovomucin liquid preparation has good adhesion to bacteria, strong resisting activity to viruses, and high solubility to the ovomucin; the effective period of the biological activity of the protein is long; microbial invasion can be effectively withstood; the activity and the toxicity of the viruses are reduced; and the ovomucin liquid preparation has a certain inhibiting effect on H5N1 and H1N1 influenza viruses, and can be sprayed on the surface of the food, animal or human mouths and noses, wounds and the like and has the functions of resisting microbial invasion and reducing the activity and the toxicity of the viruses.

Description

technical field [0001] The invention relates to an ovomucin preparation with high antimicrobial activity and a preparation method thereof. Background technique [0002] The surface antigenicity of pathogenic microorganisms will often and continuously mutate, making the process of selecting virus vaccine strains time-consuming and laborious. More importantly, the vaccination effect of the vaccine will be reduced due to the variation or drift of virus surface antigens. Antiviral chemical drugs and antibiotics are currently the most important way to treat infectious diseases, and these drugs have certain toxic and side effects on the body, and some influenza viruses and pathogenic bacteria have developed drug resistance to some drugs. Based on this situation, while developing highly effective vaccines and drugs, it has become an exciting new field to search for and excavate new natural antiviral active ingredients from abundant natural resources. [0003] Among the natural ant...

Claims

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Application Information

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IPC IPC(8): A61K38/57A61K38/40A61K38/47A61K47/26A61K47/34A61K47/18A61K47/04A61P31/04A61P31/12A01N63/50
CPCA61K38/40A61K38/47A61K9/12A61K35/57A61K36/899A61P31/04A61P31/12A01N63/50A61K2300/00A01N63/10A01N25/02
Inventor 马美湖单媛媛黄茜
Owner HUAZHONG AGRI UNIV
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