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Preparation method of maslinic acid and application of maslinic acid in preparation of antibacterial agent

The technology of an antibacterial agent and maslinic acid is applied in the fields of antibacterial and antiseptic and medicine to achieve the effects of easy storage, high antibacterial activity and low pollution

Inactive Publication Date: 2013-12-11
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Studies in recent years have shown that maslinic acid has many pharmacological activities such as anti-cancer, anti-oxidation, anti-inflammation, and anti-type 2 diabetes, but there is no report on the antibacterial activity of maslinic acid itself.

Method used

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  • Preparation method of maslinic acid and application of maslinic acid in preparation of antibacterial agent
  • Preparation method of maslinic acid and application of maslinic acid in preparation of antibacterial agent
  • Preparation method of maslinic acid and application of maslinic acid in preparation of antibacterial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: the preparation of maslinic acid in the dried fruit of Akebia clover

[0024] 1.1 Instruments and reagents

[0025] The decompression concentration adopts N-1000 rotary evaporator, CCA-1110 circulating cooling box and SB-1000 electric constant temperature water bath of Tokyo Physical and Chemical Company; HPLC adopts LC-20AT liquid chromatograph and SPD-M20A detection of Shimadzu Company device and Shim-PackPRC-ODS chromatographic column (particle size 5μm, pore size 12nm, 250mm×20mm); the medium pressure semi-preparation adopts the separation and purification system of Shanghai Lisui Technology Co., Ltd. (Dr Flash-S); the electrospray mass spectrometer (ESIMS) adopts Applied Biosystems MDS SCIEX API2000LC / MS / MS instrument, using methanol as solvent for direct sample determination; 1 H NMR spectrum and 13 The CNMR spectrum was measured using a Bruker Avance600 nuclear magnetic resonance instrument with tetramethylsilane as an internal standard. The colo...

Embodiment 2

[0036] Embodiment 2: Preparation of maslinic acid in the fresh fruit of Akebia trifoliata

[0037] 2.1 Instruments and reagents: same as Example 1

[0038] 2.2 Plant source and identification: same as Example 1

[0039] 2.3 Extraction and separation

[0040] The sample (fresh fruit of Akebia trifoliata, weighing 0.5 kg) was mashed and extracted three times with 95% ethanol at room temperature, the combined extracts were concentrated under reduced pressure to remove the organic solvent ethanol, and the crude extract of the total extract was obtained. Suspend the crude extract of the total extract in 500ml of water, then extract it with an equal volume of petroleum ether, and then extract it three times with ethyl acetate. The ethyl acetate extract is concentrated under reduced pressure to obtain the total extract of ethyl acetate (12g). Dissolve the total ethyl acetate extract with 1:1 chloroform / methanol (50mL), add normal-phase silica gel (80-100 mesh) to mix the sample at ...

Embodiment 3

[0042] Embodiment 3: Preparation of Maslinic Acid in Akebia Fruit Dried Products

[0043] 3.1 Instruments and reagents: same as Example 1

[0044] 3.2 Plant source and identification: Akebia quinata (Thumb.) Decne.

[0045] 3.3 Extraction and separation

[0046] The sample (dried Akebia fruit, weighing 0.5 kg) was crushed and extracted three times with 95% ethanol at room temperature, the combined extracts were concentrated under reduced pressure to remove the organic solvent ethanol, and the crude extract of the total extract was obtained. Suspend the crude extract of the total extract in 500ml of water, then extract it with an equal volume of petroleum ether, and then extract it three times with ethyl acetate. The ethyl acetate extract is concentrated under reduced pressure to obtain the total extract of ethyl acetate (44g). Dissolve the total ethyl acetate extract with 1:1 chloroform / methanol (100mL), add normal-phase silica gel (80-100 mesh) to mix the sample at a weight...

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Abstract

The invention provides a preparation method of maslinic acid and an application of maslinic acid in preparation of an antibacterial agent. According to the preparation method, powerful antibacterial agents (medicines) extracted and separated from akebia medicinal plants widely distributed in China are adopted, the preparation process is simple and has low cost and low contamination, the plant source is rich, the extraction is convenient, and when the fruits of the plants are extracted, the plants are not damaged and can be used for long term, so that the preparation method is environment-friendly and has good economic benefit. The monomeric compound product is stable and easy to store. Maslinic acid has wide antibacterial spectrum and high bacteriostatic activity, is much more likely to be developed into effective and safe food or vegetable and fruit antibacterial agents or new antibacterial agents capable of preventing and treating diseases caused by bacterial infection, and has a better market prospect.

Description

Technical field: [0001] The invention belongs to the technical fields of antibacterial, antiseptic and medicine, and in particular relates to a preparation method of maslinic acid and its application in the preparation of antibacterial agents. Background technique: [0002] Bacterial infection of food or fruits and vegetables is an important way for human beings to infect diseases, and the problem of drug resistance of pathogenic bacteria is also posing an increasingly significant threat to people's health. The development of effective and safe food or fruit and vegetable antibacterial agents and new anti-infective drugs has always had a realistic and extensive demand. Maslinic acid is a pentacyclic triterpene acid, which is a natural compound with low toxicity and safety to humans and the environment. Its chemical structure is shown in the formula (I) in the figure below: [0003] [0004] It has been reported in the literature that maslinic acid exists in plant materia...

Claims

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Application Information

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IPC IPC(8): C07J63/00A61K31/56A61P31/04
CPCY02A50/30
Inventor 谭建文王晶徐巧林任慧罗应
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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