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High-efficiency phosphate solubilization plant growth-promoting bacterial agent and application thereof

A plant growth-promoting bacteria and phosphorus-dissolving technology, which is applied in the field of microorganisms, can solve the problems of low utilization rate of phosphate fertilizers and decreased production increase potential, and achieve obvious growth-promoting effects and the effect of saving phosphate fertilizer resources

Active Publication Date: 2013-12-11
北京中加保罗生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention mainly aims at the problems of phosphorus deficiency, low utilization rate of phosphorus fertilizer and decreased yield increase potential in my country, and provides a widely adaptable high-efficiency phosphorus-dissolving plant growth-promoting bacteria agent

Method used

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  • High-efficiency phosphate solubilization plant growth-promoting bacterial agent and application thereof
  • High-efficiency phosphate solubilization plant growth-promoting bacterial agent and application thereof
  • High-efficiency phosphate solubilization plant growth-promoting bacterial agent and application thereof

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Experimental program
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Effect test

Embodiment 1

[0030] Isolation and cultivation of embodiment 1 Aspergillus niger C2

[0031] (1) Aspergillus niger C2 medium

[0032] Fermentation broth formula (kg / t fermentation broth): dipotassium hydrogen phosphate 0.1, potassium dihydrogen phosphate 0.1, sodium chloride 4.3, magnesium sulfate 0.1, starch 8.6, beef extract 4.3, peptone 4.3, yeast powder 4.3, sucrose 8.6, sulfuric acid Ammonium 2.6, calcium carbonate 10, ferrous sulfate 0.001, soybean oil 6.8, prepared with water.

[0033] (2) Cultivation process of Aspergillus niger C2

[0034] Inoculate Aspergillus niger C2 strain on beef juice peptone medium, culture at 28-30°C for 48h, then transfer to a 1000mL Erlenmeyer flask, and culture at 30°C, 220r / min for 36h; transfer to 50L with 5% inoculum In the seed tank, under the conditions of 220r / min, pH7.5, ventilation 0.5vvm, after culturing for 2 days, transfer it to a 500L fermenter at 200r / min, pH7.5, ventilation Under the condition of 0.7~1.0vvm, cultivate for 3 days; after t...

Embodiment 2

[0035]The preparation of embodiment 2 efficient phosphorus-dissolving plant growth-promoting bacteria agent

[0036] 1. Cultivation of Aspergillus niger C2

[0037] (1) Composition of medium (kg / t fermentation broth)

[0038] Soluble starch 10kg, soybean cake powder 10kg, corn flour 5kg, glucose 1kg, sucrose 10kg, yeast powder 5kg, (NH 4 ) 2 SO 4 3kg, K 2 HPO 4 0.2kg, NaCl2.5kg, MgSO 4 ·7H 2 O0.1kg, CaCO 3 0.5kg, FeSO 4 0.001kg, boric acid (1%) 1L, pH7.0, prepared with water.

[0039] (2) Fermentation and cultivation process

[0040] Aspergillus niger C2 was inoculated on potato sucrose (PDA) medium and cultured at 25-28°C for 3 days. Transfer the spores of Aspergillus niger C2 into a 1000mL Erlenmeyer flask, and conduct liquid fermentation at 26-28°C and 220r / min for 36h. Then insert 50L seed tank according to 1% inoculum amount, under the condition of 200r / min, pH7.0, ventilation rate 0.7vvm, cultivate after 36h, then transfer to 500L fermenter according to 10v / v...

Embodiment 3

[0046] A high-efficiency phosphorus-dissolving plant growth-promoting agent, consisting of the following components by weight:

[0047]

[0048] Wherein, the number of viable bacteria in the Aspergillus niger C2 bacterial agent is 5×10 8 -1×10 9 cfu / g.

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Abstract

The invention discloses a high-efficiency phosphate solubilization plant growth-promoting bacterial agent. The high-efficiency phosphate solubilization plant growth-promoting bacterial agent is characterized by comprising an aspergillus niger C2 bacterial agent, an additive, a binding agent and a protective agent, wherein the aspergillus niger C2 bacterial agent comprises aspergillus niger C2 with a preservation number of CGMCC NO.7511; the additive is one or more than one of silicon dioxide, kaolin, rice hull powder, wheat hull powder and straw powder; the binding agent is bentonite or water soluble microbial polysaccharide; the protective agent is one or more than one of glycerol, skimmed milk, plant oil, sodium alginate and chitosan. The high-efficiency phosphate solubilization plant growth-promoting bacterial agent is significant for saving phosphatic fertilizer resources for agricultural production in China, continuously increasing yield of grain and protecting farmland ecological environment.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a high-efficiency phosphorus-dissolving plant growth-promoting bacteria agent and its application. Background technique [0002] More than 90% of the arable land in my country is deficient in phosphorus. The use of phosphorus fertilizer is the main measure to solve crop yield increase. Phosphorus fertilizer has increasingly become a bottleneck restricting the sustainable development of my country's agriculture. my country is the world's largest consumer of chemical fertilizers and the largest consumer of phosphate fertilizers, with an annual consumption of about 11 million tons of phosphate fertilizers. As we all know, my country is short of phosphate rock resources, with reserves of 15.198 billion tons. In another 20 years, the medium and high-grade phosphate rock will be exhausted (Li Zhining, 2003a, Li Zhining, 2003b), and we have to rely on purchasing high-priced for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/00C12N1/14C09K101/00C12R1/685
Inventor 范丙全
Owner 北京中加保罗生物科技有限公司
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