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Preparation method and application of dracocephalum heterophyllum benth fractionated extracts

A kind of different leaf green orchid, graded extraction technology, applied in the field of anti-respiratory virus drugs, traditional Chinese medicine, can solve the problem of no effective preventive vaccine, can not prevent reinfection, weak immunity and other problems, achieve good medicinal prospects, rich raw materials, The effect of inhibiting viral gene replication

Inactive Publication Date: 2013-12-25
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

RSV proliferates in the epithelial cells of the respiratory tract, and bronchial necrosis adheres to mucus and fibrin, which can easily lead to airway obstruction, and can be complicated by severe bronchiolitis and pneumonia. After infection, the immunity is weak and cannot prevent reinfection. have an effective preventive vaccine

Method used

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  • Preparation method and application of dracocephalum heterophyllum benth fractionated extracts
  • Preparation method and application of dracocephalum heterophyllum benth fractionated extracts
  • Preparation method and application of dracocephalum heterophyllum benth fractionated extracts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 The preparation of the volatile oil and extract of Qinglan heterophylla

[0027] (1) Preparation of volatile oil

[0028] Take an appropriate amount of whole herb (broken into about 1 cm long) and put it into a 2000ml round-bottomed flask, steam it, distill it with water steam for 1.5 hours, and collect the distillate; use anhydrous ether to extract the distillate several times in small amounts, and collect the organic phase; Add anhydrous Na 2 SO 4 Dry the organic phase; distill the organic phase under normal pressure in the presence of zeolite, recover the distillate ether, collect the yellow oil in the flask, and obtain the volatile oil of Cynophyllum indigo; store the volatile oil at -20°C, and sterilize it by ultraviolet irradiation for 30 minutes before use .

[0029] (2) Preparation of the fractionated extract of Qinglan isophyllum

[0030] A preparation method for fractionated extract of Qinglan heterophylla, characterized in that the method compr...

Embodiment 2

[0039] Example 2 Chromography analysis of volatile oil

[0040] The components of the volatile oil of Qinglan isophyllum were analyzed by gas chromatography-mass spectrometry (Trace DSQ GC-MS instrument, American Thermo Electric Company). The gas chromatography conditions are: column DB-5ms; column length: 30m; liquid film thickness: 0.25μm; inner diameter: 0.25mm; carrier gas: He; flow rate: 1.0mL / min; 10°C / min), constant temperature for 20min; injection volume 1μL. Mass spectrometry conditions: split mode injection, split ratio 30:1; inlet temperature: 250°C; auxiliary line temperature: 280°C; ion source temperature: 250°C; ionization mode: EI; electron energy: 70eV; electron multiplier tube voltage : 1256kV; acquisition method: scanning; scanning mass range: 40~600amu.

[0041] The volatile oil was analyzed by gas-mass spectrometry, and the retention time (in sequence), name, molecular formula, relative percentage and coincidence of various components are shown in Table 1...

Embodiment 3

[0045] Example 3 In vitro antiviral effect of volatile oil and extract

[0046] (1) Cytotoxicity of the volatile oil and fractionated extracts of Cymbidium heterophylla

[0047] 1 Viruses and cell lines

[0048] Adenovirus (adenovirus, ADV3), influenza A virus H1N1, MDCK (dog kidney cells), Hep-2 (human laryngeal cancer) cells were provided by Gansu Provincial Center for Disease Control.

[0049] 2 animals

[0050] Kunming mice, half male and half male, weighing 18-22g, Medical Animal Experiment Center of Lanzhou University

[0051] 3 drugs

[0052] Use 1% DMSO as solvent to prepare the fractionated extract of the volatile oil of Indigo isophyllum, and acyclovir powder injection (Wuhan Pusheng Pharmaceutical Co., Ltd.) as a positive control

[0053] Cells were cultured, and the volatile oil and extract were serially diluted to 5 dosage concentrations (4mg / ml, 2mg / ml, 1mg / ml, 0.5mg / ml, 0.25mg / ml) with cell maintenance solution, and added to the cells already In a 96-well p...

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Abstract

The invention relates to a preparation method and an application of dracocephalum heterophyllum benth fractionated extracts. Compared with the existing anti-virus synthetic medicines, the dracocephalum heterophyllum benth fractionated extracts have low toxicity, are safe and reliable and have strong pharmaceutical effect; and the dracocephalum heterophyllum benth raw materials are abundant and cheap, the extraction process is simple and the preparation cost is low, so the dracocephalum heterophyllum benth fractionated extracts can be prepared into various dosage forms.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and relates to an anti-respiratory tract virus drug, in particular to the application of the volatile oil of Cymbidium heterophylla in the preparation of anti-respiratory tract virus drug. Background technique [0002] Tibetan medicine Dracocephalum heterophyllum Benth is the dry aboveground part of Dracocephalum heterophyllum Benth, a perennial herb, also known as Baihuazhizihua, Baihua Prunella, Jiziqingbao (Tibetan medicine name), Xinjiang Uighur name Marzan Jusi, Zupar. The aerial part of Chrysanthemum variegata has the effect of clearing and purging liver heat, and it is a traditional medicine used by Uighurs and Tibetans to treat cough, asthma and stomach problems. It is mainly used to treat various diseases such as icteric fever, liver heat, febrile headache, cloudy eyes, oral ulcers, bronchitis, and high blood pressure. It is a species included in the standards issued by the ...

Claims

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Application Information

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IPC IPC(8): A61K36/53A61P31/12A61P31/16A61P31/20A61P11/00
Inventor 张春江
Owner LANZHOU UNIVERSITY
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