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Biomedical polysaccharide hemostatic and healing sponge and preparation method thereof

A technology of biological polysaccharides and sponges, applied in the field of medical materials, can solve the problems of poor mechanical strength and stability of cross-linked products, slow wound healing speed, poor hemostatic effect of sponges, etc., achieve soft texture, accelerate wound healing, and have no toxic side effects Effect

Active Publication Date: 2014-01-01
JIANGSU CHANGJIYONG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] In order to solve the problems in the above hemostatic sponge cross-linking technology that the material is single, the mechanical strength and stability of the obtained cross-linked product are poor, so that the hemostatic effect of the sponge is poor, the speed of promoting wound healing is slow, and the problem that cannot meet the clinical requirements is provided by the present invention. A natural biopolysaccharide hemostatic healing sponge with easy-to-obtain, strong flexibility, good stability, no toxic and side effects, no stimulation, rapid hemostasis, trauma and surgical hemostasis for absorbing exudate, and promotion of wound healing and its preparation method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Preparation of DVS cross-linked HA sponge

[0024] Take 1.2 g of sodium hyaluronate (HA) and dissolve it in 30 mL of 0.02M NaOH alkaline aqueous solution to obtain a 4% (W / V) sodium hyaluronate solution, swell for 5 hours to obtain a colloid, add crosslinking agent divinyl Sulfone (DVS) 30μL, stir and mix well, incubate at 35°C for 6h to obtain a uniform gel, and place the obtained gel in 0.1M NaCl solution and 40% (V / V) ethanol solution for alternate purification for 48h Add 0.2% glycerin to the purified gel, stir and bubble at high speed, pour it into a freeze-dried tray, pre-freeze in a freeze dryer at -45°C for 8 hours, and then perform low-temperature sublimation at -15°C for 32 hours to obtain a white sponge solid. Sealed and packaged in paper-plastic bags and sterilized by Co-60γ-ray irradiation.

Embodiment 2

[0025] Example 2 Preparation of DVS cross-linked CMC-HA composite sponge

[0026] Mix 1.2 g of sodium carboxymethyl cellulose (CMC) and 0.3 g of sodium hyaluronate (HA), and dissolve them in 30 mL of an alkaline aqueous solution of NaOH with a concentration of 0.02M to obtain 5% (W / V) CMC- The HA mixed solution was swelled for 5 hours to obtain a colloid. Add 150μL of the cross-linking agent divinyl sulfone (DVS), stir well, and incubate at 35°C for 6 hours to obtain a uniform gel. Place the obtained gel in 0.1M NaCl The solution is purified alternately with 40% (V / V) ethanol solution for 48 hours, 0.2% glycerol is added to the purified gel, stirred at high speed, poured into a freeze-dried tray, and pre-freeze at -45°C for 8 hours in a freeze dryer. Sublimation at -15°C for 32 hours is carried out, and the white sponge solid obtained is sealed and packaged in a paper-plastic bag, and subjected to Co-60 γ-ray irradiation sterilization treatment.

Embodiment 3

[0027] Example 3 Preparation of BDDE cross-linked CMC sponge

[0028] Take 1.2 g of sodium carboxymethyl cellulose (CMC) and dissolve it in 30 mL of 0.04M NaOH alkaline aqueous solution to obtain a 4% (W / V) CMC solution. Swell for 5 hours to obtain a colloid. Add crosslinking agent 1. 4~1.2 mL of butanediol diglycidyl ether (BDDE), stir well, incubate at 45°C for 8h to obtain a uniform gel, soak the obtained gel in 0.1M NaCl solution for 48h, and then use it for purification Purify by water dialysis for 8 hours, add 0.3% glycerol to the purified gel, stir and bubble at high speed, pour it into a freeze-dried tray, pre-freeze in a freeze dryer at -45°C for 10 hours, and then perform low temperature sublimation at -15°C for 40 hours. The white sponge solid is sealed and packaged in aluminum foil bags, and subjected to Co-60 γ-ray irradiation sterilization treatment.

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Abstract

The invention discloses biomedical polysaccharide hemostatic and healing sponge and a preparation method thereof, and belongs to the technical field of medical materials. The preparation method comprises the following steps: firstly, dissolving a polysaccharide material carboxymethyl cellulose, hyaluronic acid or a mixture thereof into an alkaline solution, stirring sufficiently, and swelling into colloid; secondly, adding a crosslinking agent into the colloid, stirring sufficiently, incubating into uniform gel, and dialyzing and purifying the obtained gel; thirdly, adding glycerin into the purified product, stirring at a high speed for bubbling, and pouring into a lyophilizing disk; and finally, performing freeze drying at a low temperature to obtain dehydrated sponge. The hemostatic and healing sponge prepared with the method is good in flexibility, do not drop crumbs, is strong in liquid absorption, can provide a wet environment to a wound, is non-stick to the wound, is good in biocompatibility, is qualified in heavy metal detection and endotoxin detection, is non-cytotoxic in a bio-safety test, is free of intradermal irritation and sensitization response, and has the functions of stopping bleeding rapidly, absorbing exudate and promoting wound healing.

Description

technical field [0001] The invention relates to a medical biological polysaccharide hemostatic and healing sponge and a preparation method thereof, in particular to a sponge capable of rapid hemostasis, absorbing seepage fluid and promoting wound healing and a preparation method thereof, belonging to the technical field of medical materials. Background technique [0002] The traditional hemostatic materials used clinically are gauze, cotton pads, etc., but the hemostatic effect of traditional methods is not satisfactory. In recent years, hemostatic sponges have been widely used in internal and external wounds and surgical hemostasis, which can quickly stop bleeding and promote wound healing. At present, most hemostatic sponge products are made of collagen sponge, gelatin sponge, chitosan and chitosan sponge, while hemostatic sponges made of hyaluronic acid and carboxymethyl cellulose materials are rare, and the two materials are natural polysaccharides. Biomaterials have go...

Claims

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Application Information

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IPC IPC(8): A61L15/28
Inventor 笪利王风华吕园园
Owner JIANGSU CHANGJIYONG BIOTECH CO LTD
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