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Method for producing methane by using anerobic fermentation of rumen microorganism

A rumen microorganism and anaerobic fermentation technology, which is applied in the field of methane production by rumen microorganism anaerobic fermentation, can solve the problems such as the preparation of methane gas that has not been seen, and achieve the effects of solving the problem of intractable treatment, shortening the hydraulic retention time and improving the degradation efficiency.

Active Publication Date: 2015-04-08
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this method, the straw is first crushed, and then intermittently inserted into an anaerobic fermentation reactor inoculated with rumen microorganisms to degrade the biomass waste. The main products are volatile organic acids and carbon dioxide, and there is no report on the production of methane gas.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0019] 1. Collect the corn stalks after harvesting, and cut the stalks to 20-30mm sections with a cutting machine.

[0020] 2. Spray the anaerobic fermentation biogas slurry of urban sludge with an ammonia nitrogen content of 1000mg / L on the surface of the cut straw. The spray weight of the biogas slurry is 40% of the dry weight of the biomass waste. Stir evenly and ammoniate in a closed container .

[0021] 3. The temperature of the pretreatment process is controlled at 40°C, and the straw pretreatment process is completed after taking out the straw after 3 days.

[0022] 4. An anaerobic fermentation reactor with an effective volume of 4 liters is equipped with 100 g of straw (dry weight) and 3.6 L of buffer solution. First, nitrogen is aerated at a rate of 1 L / min·L for 10 minutes to drive off the residual oxygen in the reactor. Inoculate 400 mL of rumen liquid obtained from the fresh rumen of beef cattle slaughtered in the slaughterhouse into the anaerobic fermentation rea...

example 2

[0025] 1. Collect organic domestic waste and remove the inorganic substances contained in it, such as glass, plastic bags, stones, etc. The pulverizer pulverizes into particles smaller than 8mm.

[0026] 2. Spray the biogas slurry of rumen microbial anaerobic fermentation biomass waste with an ammonia nitrogen content of 600mg / L on the crushed organic domestic garbage. The spray weight of the biogas slurry is 80% of the dry weight of the garbage. change.

[0027] 3. The temperature of the pretreatment process is controlled at 18°C. After 6 days, the biomass organic domestic waste is taken out, and the pretreatment process of the organic waste is completed, and the raw materials are used for anaerobic fermentation.

[0028] 4. An anaerobic fermentation reactor with an effective volume of 4.5 liters is equipped with 200 g of biomass organic domestic waste and 2.3 L of buffer solution. First, argon is exposed at a rate of 1.5 L / min·L for 10 minutes to drive off the residual oxyg...

example 3

[0032] 1. Collect water hyacinth. Use a crusher to crush the water hyacinth to particles smaller than 5mm.

[0033] 2. Spray the pig manure anaerobic fermentation biogas slurry with an ammonia nitrogen content of 4000mg / L on the surface of the crushed water hyacinth. The weight of the biogas slurry sprayed is 140% of the dry weight of the biomass waste. Stir evenly and ammoniate in a closed container.

[0034] 3. The temperature of the pretreatment process is controlled at 25°C. After 10 days, the water hyacinth particles are taken out, and the pretreatment process of the water hyacinth is completed, and the raw materials are used for anaerobic fermentation.

[0035] 4. An anaerobic fermentation reactor with an effective volume of 3.5 liters is equipped with 50 g of water hyacinth particles and 2.6 L of buffer solution. Firstly, the micropores are exposed to helium at a rate of 2 L / min L for 20 minutes to remove the residual oxygen in the reactor. Inoculate the anaerobic ferme...

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Abstract

The invention relates to a method for pretreatment to biological substance waste materials and the biotechnology of resource utilization of the biological substance waste materials. Firstly, biogas slurry is used for pretreatment to the biological substance waste materials, and then rumen microorganism is used for anaerobic biotransformation; the fermentation process is that a rumen fluid is taken out of rumina of ruminants and inoculated into an anerobic fermentation reactor containing biological substance waste materials subjected to pretreatment and a buffer solution; the anerobic fermentation reactor runs to collect produced methane, and liquid phase and solid phase products. According to the invention, the dry matter digestion rate of biological substance waste material is improved by 10 to 20 percent, the fermentation time is shortened for 3 to 10 days, and the methane producing efficiency can reach 210 to 250 mL / g (moisture free weight). The biogas liquid produced by fermentation can be used for pretreatment in a recyclable way, and also can be used as an organic fertilizer after biogas residue treatment.

Description

technical field [0001] The invention belongs to the technical field of pretreatment of biomass waste, and relates to a method for producing methane by anaerobic fermentation of rumen microorganisms. Background technique [0002] The biomass waste referred to in the present invention mainly refers to agricultural straw, organic domestic garbage, aquatic plants, etc., wherein agricultural straw includes stems and stems of plants such as corn, wheat, sorghum, rice, and potatoes, and the annual output of various agricultural crop straws in my country Just above 1 billion tons, most of the existing treatment methods are incineration on the spot, which not only causes serious air pollution, but also wastes precious resources. As one of the final products of anaerobic fermentation, biogas slurry has the characteristics of rich nitrogen content, but it is difficult to deal with by ordinary methods. From the perspective of environmental protection, how to reduce and recycle biomass w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P5/02
CPCY02E50/30
Inventor 杨凤林靳文尧徐晓晨
Owner DALIAN UNIV OF TECH
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