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A hybrid artificial liver based on reciprocating bioreactor loaded with microcapsules

A bioreactor, artificial liver technology, applied in suction devices, dialysis systems, etc., can solve the problems of low plasma passing rate, low capsule filling rate, and low fluidization rate in fluidized bed reactors, avoiding ineffective perfusion, Improve mixing effect and avoid the effect of perfusion dead space

Inactive Publication Date: 2016-09-14
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the volume of the fluidized bed reactor is relatively large, the filling rate of the capsule is low, and a large cavity volume is required to achieve fluidization; in addition, due to the limitation of the physical properties of the microcapsule or gel beads, the fluidization rate is low. Plasma throughput in fluidized bed reactors is very low

Method used

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  • A hybrid artificial liver based on reciprocating bioreactor loaded with microcapsules
  • A hybrid artificial liver based on reciprocating bioreactor loaded with microcapsules
  • A hybrid artificial liver based on reciprocating bioreactor loaded with microcapsules

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Experimental program
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Effect test

Embodiment 1

[0026] The blood of patients with liver failure passes through the plasma separator 1, is pumped into the physical artificial liver circuit by the peristaltic pump 2, passes through the hemodialyzer 4, removes some toxins, and then enters the blood storage pool 5, during which the heparin pump 3 continuously injects heparin; the blood storage pool 5. The volume is 50-500ml. The upper end is connected to the atmosphere through the air filter membrane. It contains a membrane oxygenator. Connection, so the two circulation paths are relatively independent, and the flow and flow direction do not interfere with each other. Plasma is pumped into bioreactor 8 (volume 200-1000ml) by peristaltic pump 7, and performs material exchange with microencapsulated hepatocytes (particle size 100-1000μm; loading volume accounts for 50%-100% of reactor volume), reactor 8 There are 50-100μm soft screens at the upper and lower ports, which can prevent the microcapsules from being taken out of the re...

Embodiment 2

[0028] Preparation of hepatic cell aggregates: Hepatocytes obtained by perfusion or mechanical methods are subjected to limited digestion to form cell clusters, or digested single cells are rotated to form cell clusters of 100-300 μm.

[0029] Preparation of microencapsulated hepatocytes: suspension of cell clusters and sodium alginate, cell density 1×10 7 -1×10 8cells / ml, drop into the calcium solution through a high-voltage electrostatic preparation device, and the formed calcium alginate beads react with polylysine or chitosan solution to form a film. The laboratory self-made multi-channel preparation instrument can prepare 100ml of microencapsulated hepatocytes in 10 minutes, reducing the damage to cell activity during the preparation process.

[0030] Bioreactor: The design volume of the reactor is 200-2000ml, the diameter-to-height ratio is less than 0.1-10, and the upper and lower ports have soft screens of 50-100μm. Microencapsulated cells are loaded from the upper en...

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Abstract

The invention discloses a mixed type artificial liver supporting system comprising two circulation passages. The mixed type artificial liver comprises a reciprocating type bioreactor filled with microencapsulation hepatic cells, a plasma separator, a blood storage pond, a hemodialyzer and a membrane type oxygenator. Blood sequentially enters the plasma separator, the hemodialyzer and the blood storage pond and then enters the bioreactor through a peristaltic pump, and the contradiction between the non-tolerance of the human body to extracorporeal high-flowing-speed circulation of blood liquid and the high-passing-rate requirement of bioartificial liver plasma is effectively solved. Reciprocating type flowing direction changing is adopted on the bioartificial liver passages, backlash is achieved, the mixing effect is improved, flowing blocking caused by the fact that microcapsules are accumulated at one end of the bioreactor can be effectively reduced, the internal pressure of the bioreactor is reduced, microcapsule damage caused by continued pressurizing is reduced, good food mixing can be achieved under the conditions of a high flow speed and a high liver cell density, hepatic failure plasma makes full contact with the liver cells, potting dead space and invalid potting are effectively avoided, the number of damage caused by shearing is small, and the therapeutic action of the artificial liver can be achieved to the maximum extent.

Description

technical field [0001] The invention relates to a medical device, in particular to a hybrid artificial liver support system for clinical treatment of acute liver failure. Background technique [0002] Acute liver failure is a common critical disease with a mortality rate as high as 65-95%. At present, liver transplantation is the only effective way to treat acute liver failure, but there is a serious shortage of liver sources. Most patients die while waiting for liver sources. It is necessary to develop an extracorporeal artificial liver support system to temporarily compensate the liver function, help the patient's own liver to recover and regenerate, or help the patient bridge the transition to the liver transplantation period. The bioartificial liver with highly active liver cells can effectively perform functions such as liver synthesis and transformation. At the same time, in order to reduce the damage of metabolites to liver cells and strengthen the detoxification func...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61M1/16
CPCA61M1/16A61M1/1698
Inventor 马小军陈立张英于炜婷李娜马颖李珅王雨
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI