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A method for separating and extracting L-serine from Corynebacterium glutamicum fermentation broth

A technology of Corynebacterium glutamicum and fermentation broth, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve problems such as separation difficulties, achieve high extraction efficiency, be beneficial to comprehensive utilization, and be easy to realize in industrialization Effect

Inactive Publication Date: 2015-08-12
GUANGDONG HUANXI BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the hydrolyzate, its system composition is relatively simple, and there is no D-type amino acid, but valine, alanine and serine are relatively close in nature, and separation is more difficult

Method used

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  • A method for separating and extracting L-serine from Corynebacterium glutamicum fermentation broth
  • A method for separating and extracting L-serine from Corynebacterium glutamicum fermentation broth
  • A method for separating and extracting L-serine from Corynebacterium glutamicum fermentation broth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Flocculation: Take 50 L of Corynebacterium glutamicum fermentation broth, adjust the pH of the fermentation broth to 3.0 with hydrochloric acid, and the liquid temperature is 30°C, add 0.5 L of 20g / L polyacrylamide solution, stir for 40 minutes, let it stand for one hour, and then centrifuge. The precipitate was removed to obtain 47.5L of supernatant. After testing, the removal rate of bacteria by polyacrylamide flocculation was 98.49%;

[0055] Decolorization: Add 0.5 kg of powdered activated carbon to the above liquid, heat up to 60°C, stir for 30 minutes to decolorize, filter to remove the activated carbon, and obtain 46L of decolorized liquid. After testing, the decolorization rate of activated carbon is 92.73%. Through flocculation and decolorization operations, L- The yield of serine is 90.2%;

[0056] Ultrafiltration: Roll-type ultrafiltration membrane test device (PVDF membrane, filtration area 0.5m 2 ) Perform ultrafiltration on the obtained 46L decolori...

Embodiment 2

[0062] flocculation : Take 50 L of Corynebacterium glutamicum fermentation broth, adjust the pH of the fermentation broth to 5.0 with hydrochloric acid, and adjust the liquid temperature to 40°C, add polyacrylamide to adjust its concentration to 150 mg / L, stir for 60 minutes, and centrifuge after standing for 3 hours , remove the precipitate to obtain the clear liquid;

[0063] bleaching : Add powdered activated carbon to the above clear liquid in an amount of 20g / L, heat up to 50°C, stir and decolorize for 60min, filter and remove the activated carbon to obtain a decolorized solution;

[0064] ultrafiltration : The roll-type ultrafiltration membrane test device is used to carry out ultrafiltration on the decolorization liquid, and its molecular weight cut-off is 5000Da. When the remaining 5-8L of the decolorization liquid to be intercepted is left, add 5-10L of purified water to wash, wash for 3 times, and combine Obtain ultrafiltrate;

[0065] Adsorption and Elution...

Embodiment 3

[0070] flocculation : Take 50 L of Corynebacterium glutamicum fermentation broth, adjust the pH of the fermentation broth to 4.0 with hydrochloric acid, and adjust the liquid temperature to 30°C, add polyacrylamide to adjust its concentration to 10 mg / L, stir for 90 minutes, and centrifuge after standing for 6 hours , remove the precipitate to obtain the clear liquid;

[0071] bleaching : Add powdered activated carbon to the above clear liquid in an amount of 5g / L, heat up to 65°C, stir for 60 minutes to decolorize, filter to remove the activated carbon, and obtain a decolorized solution;

[0072] ultrafiltration : Ceramic ultrafiltration membrane test device is used to carry out ultrafiltration on the decolorization liquid, and its molecular weight cut-off is 2000Da. When the remaining 5-8L of the decolorization liquid to be intercepted is left, 5-10 L of purified water is added to wash, and the washing operation is performed 3 times, and combined to obtain ultrafiltrat...

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Abstract

The invention discloses a method for separating and extracting L-serine from Corynebacterium glutamicum fermentation liquid, which comprises the following steps: flocculating the fermentation liquid, decolorizing the extract after solid-liquid separation through active carbon adsorption, and decolorizing by ultrafiltration. In addition to macromolecules, cationic resin is used to adsorb amino acids, and ammonia water is used for elution. The eluate was concentrated in vacuo, and ethanol was added for cooling crystallization to obtain L-serine crystals. The method of the invention has high extraction efficiency, and the single-pass extraction rate can reach more than 56%, and the comprehensive extraction rate can reach more than 80% through recovery and reprocessing of the mother liquor. The method of the invention is easy to implement industrially, the extraction cost is relatively low, and it can be processed in large batches.

Description

technical field [0001] The invention relates to an extraction process, in particular to a method for extracting L-serine from the fermentation liquid of Corynebacterium glutamicum. Background technique [0002] L-serine (L-serine, abbreviated as L-ser), the molecular formula is C 3 h 7 NO 3 , the chemical structure is HO-CH 2 CH(NH 3 )-COOH, molecular weight 105.10, chemical name α-amino-β-hydroxypropionic acid. L-serine is a non-essential amino acid, which is a sugar-forming amino acid and plays an important role in the process of cell proliferation. During cell growth, L-serine is the main one-carbon unit donor for the synthesis of purine nucleotides and deoxythymidine phosphate. It is also a precursor of other amino acids (such as cysteine, taurine), lipid messenger molecules (such as phosphatidylserine, ceramide) and neuromodulators (glycine, D-serine). L-serine is widely used in the food industry, medicine, and cosmetics, and its market demand continues to increas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C229/22C07C227/40
Inventor 许正宏史劲松郭雯方佳茂陈伟滨张晓梅罗玉常朱勤建
Owner GUANGDONG HUANXI BIOLOGICAL TECH
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