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Enzyme and bacterium complexing agent for degrading crop straws
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A technology of crop straw and compounding agent, which is applied in application, animal feed, animal feed, etc., can solve the problems of insufficient carbon and nitrogen, high fiber degradation effect, and degradation effect not as good as fungus, etc., to ensure effective degradation and improve availability , the effect of shortening the fermentation time
Active Publication Date: 2015-02-04
FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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Problems solved by technology
Fungi have a good decomposing effect on cellulose and lignin, and although bacteria also have the corresponding ability to produce cellulase, the degradation effect is not as good as that of fungi, especially in straw fermentation, when straw lignin has not been degraded, bacteria The amount of carbon and nitrogen required for growth and expansion is insufficient to maintain the activity of bacteria
At the same time, too many strains are used, which not only increases the cost, but also does not guarantee a high fiber degradation effect
[0017] Existing straw-decomposing bacteria have the problem of long fermentation period, such as CN200710061529.4 needs to be fermented for 15 days, CN201210178963.1 needs to be fermented for 4-25 days; although the fermentation time is shortened, some straws need to be physically pretreated, which increases the cost. For relatively cheap straw, the gain outweighs the gain. For example, although the fermentation time of CN201110441198.3 is 2 days, the straw needs to be soaked in ammonia water for 24-48 hours in advance; 3058337-0019-0003 data adopts steam explosion treatment
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preparation example Construction
[0063] The composition of the PDA medium is: potato extract 1L, glucose 20g, KH 2 PO 4 3g,MgSO 4 1.5g, vitamin B 1 10mg, agar 15g, pH6.0. The preparation method of the potato extract comprises the following steps: weighing 200g of potatoes, washing, peeling, cutting into small pieces, adding 1000mL of water, boiling for 30min, filtering with gauze to obtain a clear liquid, adding water to 1000mL.
[0064] Other carriers, feed additives, and raw materials can be purchased from the feed market.
Embodiment 1
[0065] Example 1: An enzyme-bacteria compound agent for degrading crop stalks
[0066] 1. Composition: 45 parts of microbial compound agent, 10 parts of compound enzyme, 45 parts of carrier. in:
[0067] Microorganism complex agent: 25 parts of Phanerochaetechrysosporium, 25 parts of Aspergillus niger, 25 parts of Penicillium, 25 parts of Trichoderma viride; each part contains 25ml culture solution, and the number of viable bacteria in the culture solution is 10 8 individual / mL.
[0076] Example 2: An enzyme-bacteria compound agent for degrading crop stalks
[0077] 1. Composition: 30 parts of microbial compound agent, 15 parts of compound enzyme, and 55 parts of carrier.
[0078] Microorganism complex agent: 33 parts of Phanerochaetechrysosporium, 33 parts of Aspergillus niger, 33 parts of Penicillium; each part of bacteria contains 25ml culture solution, and the number of viable bacteria in the culture solution is 10 8 individual / mL.
[0080] Carrier: 50 parts of glucose, 50 parts of calciumchloride.
[0081] 2, preparation method: with embodiment 1.
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Abstract
The invention relates to an enzyme and bacterium complexing agent for degrading crop straws. The enzyme and bacterium complexing agent comprises the active components including a microorganism complexing agent and compound enzyme. An experiment result shows that xylogen, neutral detergent fibers, acid detergent fibers, acid detergent xylogen, cellulose, crude protein and the like of the straws treated by the enzyme and bacterium complexing agent are well degraded; the degrading rate of rumens of ruminants to the xylogen of the straws is increased, and the surrounding of the xylogen around other nutritional components is broken through, so that the availability of the straws is improved. The enzyme and bacterium complexing agent disclosed by the invention is convenient to use; the straws are not required to be subjected to physical pretreatment; the fermentation time of the straws is short; an effect of the complexing agent is better than that in the prior art.
Description
technical field [0001] The invention relates to the field of processing and utilization of ruminant roughage in the feed industry, in particular to an enzyme-bacteria compound agent for degrading crop straws. Background technique [0002] my country is a large agricultural country with extremely rich cropstraw resources, and the world's annual straw output is as much as 3 billion tons. In 2009, the remaining straw output after crop harvest in my country was as high as 740 million tons, ranking among the top in the world. However, due to the particularity of the physical and chemical properties of crop straw, it has many limitations when used as feed. Straw is relatively rough in texture, poor in palatability, poor in nutritional value, high in lignin and cellulose, and low in digestion and utilization. Only a small part can be used to feed ruminants. Monogastric animals generally cannot use straw, and the digestibility of straw by ruminants is only 20%-30%. How to improv...
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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/14A23K1/00A23K1/165
Inventor 刁其玉屠焰张立霞
Owner FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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