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Soporific and sedative drug inspection method for treating biological detection material by using gel permeation chromatography

A technology of gel permeation chromatography and biological testing materials, which is applied in the field of hypnotic and sedative drug testing, can solve the problems of no systematic testing methods, lack of effective and feasible methods, etc., and achieve shortened preprocessing time, reduced analysis costs, and high sensitivity Effect

Active Publication Date: 2015-04-15
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since it is difficult to remove macromolecular impurities such as proteins and oils contained in biological samples such as blood, urine, and liver tissue, there is no systematic test method for certain types of toxic substances in biological samples such as blood, urine, and liver tissue.
In the analysis and detection of toxicants in the domestic public security system, the application of gel permeation chromatography is still in its infancy, and there is still a lack of effective and feasible methods.

Method used

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  • Soporific and sedative drug inspection method for treating biological detection material by using gel permeation chromatography
  • Soporific and sedative drug inspection method for treating biological detection material by using gel permeation chromatography
  • Soporific and sedative drug inspection method for treating biological detection material by using gel permeation chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Biological sample preparation

[0025] Blank blood and blank urine were collected from healthy volunteers who did not take any drugs within a week; blank liver tissues were collected from pigs that did not take any drugs within a week. Diazepam, estazolam, alprazolam, triazolam, chlorpromazine and promethazine were all purchased from the National Standard Materials Research Center.

[0026] 2. Experimental conditions

[0027] 2.1 Preparation of standard solution

[0028]Preparation of standard stock solutions: Weigh 10 mg each of diazepam, estazolam, alprazolam, triazolam, chlorpromazine and promethazine standard substances, place them in 10mL volumetric flasks, add a small amount of Dissolve in methanol and adjust the volume to the mark, prepare 1 mg / mL standard stock solutions of diazepam, estazolam, alprazolam, triazolam, chlorpromazine and promethazine respectively, and store in refrigerator at 4°C ,spare.

[0029] Pipette a certain amount of individual hypno...

Embodiment 2

[0108] The difference between this embodiment and Example 1 is that the extraction solvent (i.e. organic solvent A) is a mixed solution of cyclohexanol and propyl butyrate, and the volume ratio of cyclohexanol and propyl butyrate in the mixed solution is 2:11; the remaining experimental conditions All are the same; using the mixed solution of cyclohexanol and propyl butyrate as the extraction solvent, the addition recovery and precision of the six hypnotic and sedative drugs are shown in Table 3-11~3-13.

[0109] Table 3-11 Addition recovery and precision of 6 hypnotic and sedative drugs in blood after GPC purification

[0110]

[0111] Table 3-12 Addition recovery and precision of 6 hypnotic and sedative drugs in urine after GPC purification

[0112]

[0113] Table 3-13 Addition recovery and precision of 6 hypnotic and sedative drugs in the liver after GPC purification

[0114]

[0115]

[0116] It can be seen from Tables 3-11 to 3-13 that with the mixed solution...

Embodiment 3

[0118] The difference between this embodiment and Example 1 is that the extraction solvent (i.e. organic solvent A) is a mixed solution of isopentyl ether and cycloheptane, and the volume ratio of isopentyl ether and cycloheptane in the mixed solution is 3:5; The experimental conditions were all the same; using the mixed solution of isopentyl ether and cycloheptane as the extraction solvent, the recovery rates and precision of the addition of the six hypnotic and sedative drugs are shown in Tables 3-14 to 3-16.

[0119] Table 3-14 Addition recovery and precision of 6 hypnotic and sedative drugs in blood after GPC purification

[0120]

[0121] Table 3-15 Addition recovery and precision of 6 hypnotic and sedative drugs in urine after GPC purification

[0122]

[0123] Table 3-16 Addition recovery and precision of 6 hypnotic and sedative drugs in the liver after GPC purification

[0124]

[0125] It can be seen from Tables 3-14 to 3-16 that the recoveries of biological...

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Abstract

The present invention discloses a soporific and sedative drug inspection method for treating a biological detection material by using a gel permeation chromatography. The method comprises the following steps: (1) adding an organic solvent A to a biological detection material containing a soporific and sedative drug, carrying out centrifugation, separating the organic phase, concentrating the obtained organic phase until drying, dissolving with the organic solvent A, and filtering with an organic microporous filtration membrane to obtain the extraction solution; (2) adding the extraction solution to a gel permeation chromatography purification column, carrying out elution with an organic solvent B, collecting the eluent, carrying out blowing drying through a concentrator, and dissolving with an organic solvent C to obtain a solution requiring detection, wherein the filling phase of the gel permeation chromatography purification column is a styrene-divinylbenzene copolymer; and (3) detecting the solution requiring detection by using a gas chromatography. According to the present invention, the gel permeation chromatography technology is applied in the field of toxicant analysis, the biological detection material pretreatment technology with characteristics of simpleness, rapidness, high sensitivity and high recovery rate is established based on the soporific and sedative drug, advantages of pretreatment time shortening, analysis cost reduction and detection result reliability increase are provided, problems of various detection material types and complex components in the toxicant analysis are solved, and assurance is provided for improvement of efficiency of the toxicant analysis inspection work in the public security industry.

Description

technical field [0001] The invention relates to a drug and poison detection method in the field of criminal investigation, in particular to a hypnotic and sedative drug detection method using gel permeation chromatography to process biological samples. Background technique [0002] With the advancement of technology, the requirements for drug and toxicant analysis and testing are getting higher and higher, including high reproducibility, high recovery rate, low detection limit and simple operation, etc., so it is urgent to establish a simple, fast and sensitive and a pretreatment method with a high recovery rate. At present, the existing pretreatment technologies mainly include liquid-liquid extraction, solid-phase extraction, and solid-phase microextraction. Check the situation. In the sample pretreatment technology, gel permeation chromatography technology, as a new type of analysis technology, has shown its potential application prospects due to its strong regeneration ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 栾玉静王瑞花董颖张蕾萍杜鸿雁常靖王芳琳王炯张云峰侯小平于忠山何毅崔冠峰
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY