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Preparation of magnetic fluorescent bifunctional microspheres by connecting polyaniline magnetic microspheres with fluorescent quantum dots

A fluorescent quantum dot, dual-functional technology, applied in the field of magnetic fluorescent dual-functional microspheres, can solve the problems of limitations, inability to separate and apply fluorescent markers, etc.

Inactive Publication Date: 2016-02-24
SUZHOU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the in-depth development of magnetic nanomaterials, a single magnetic property cannot meet biological applications. It is the general trend to combine magnetic and fluorescent properties, which can solve magnetic separation and fluorescent labeling, and solve the problem of single fluorescent markers after labeling. Difficulties that cannot be separated and are greatly limited in application

Method used

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  • Preparation of magnetic fluorescent bifunctional microspheres by connecting polyaniline magnetic microspheres with fluorescent quantum dots
  • Preparation of magnetic fluorescent bifunctional microspheres by connecting polyaniline magnetic microspheres with fluorescent quantum dots
  • Preparation of magnetic fluorescent bifunctional microspheres by connecting polyaniline magnetic microspheres with fluorescent quantum dots

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1Fe 3 o 4 Preparation of / CdSe / CdS Magnetic Fluorescent Bifunctional Nanocomposite Microspheres

[0050] 1. Prepare fluorescent quantum dots, and control the particle size to 2-10nm.

[0051] Taking cadmium selenide wrapped cadmium sulfide (CdSe / CdS) as an example, cadmium selenide wrapped cadmium sulfide (CdSe / CdS) was synthesized and modified. The specific preparation method is as follows:

[0052] CdSe quantum dots were synthesized in oil phase by literature method (Diao, X.L.; Xia, Y.S.; Zhang, T.L.; Li, Y.; Zhu, C.Q.J. Anal. Bioanal. Chem. 2007, 388, 1191). A certain amount of CdO was added to oleic acid and liquid paraffin, put into a three-neck flask and heated and stirred, and the CdO was dissolved at 150°C to form a uniform light yellow Cd solution. Put Se powder into liquid paraffin and stir quickly, heat to 220°C (in another three-neck flask), the solution first turns orange and then red. Gently inject the Cd solution into the Se solution while st...

Embodiment 2

[0061] Example 2 Fe 3 o 4 Preparation of / CdTe Magnetic Fluorescent Bifunctional Nanocomposite Microspheres

[0062] The specific steps are roughly the same as those in Example 1, except that the quantum dots used are CdTe modified with thioglycolic acid.

[0063] (1) The preparation method of CdTe modified with thioglycolic acid is as follows:

[0064] 1. Preparation of Te precursor

[0065] Take 80mgTe powder and 50mgNaBH respectively 4 Add it to the vial, close the mouth of the bottle, and inject 1ml of distilled water with a syringe. Seal the mouth of the bottle with raw tape, and then insert a needle into the bottle stopper to make the H produced by the reaction 2 drain out. React the vial at 0°C for 8 hours, it can be observed that the black Te powder disappears, the upper layer is a colorless NaHTe solution, and the lower layer is NaHTe 2 B 4 o 7 White precipitate.

[0066] 2. Preparation of Cd precursor

[0067] Take 0.2854g (1.25×10-3mol) CdCl 2 2.5H 2 O ...

Embodiment 3

[0073] Example 3 Fe 3 o 4 Properties of / CdSe / CdS Magnetic Fluorescent Bifunctional Nanocomposite Microspheres

[0074] The present embodiment obtains Fe with embodiment 1 3 o 4 / CdSe / CdS magnetic fluorescent bifunctional nanocomposite microspheres as an example to study some properties of the obtained nanocomposite microspheres. The luminescence and magnetic properties of fluorescent magnetic bifunctional microspheres were studied by transmission electron microscope, fluorescence microscope and magnetic property measurement.

[0075] image 3 It is the energy spectrum of the magnetic fluorescent bifunctional nanocomposite microsphere. Figure 4 for Fe 3 o 4 / CdSe / CdS magnetic fluorescent bifunctional nanocomposite microspheres real-time distribution photos and fluorescence microscope photos. Figure 5 for Fe 3 o 4 / CdSe / CdS magnetic fluorescent bifunctional nanocomposite microspheres transmission electron micrographs. The appearance and particle size of CdSe / CdSQDs...

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Abstract

The invention discloses a magnetic fluorescent bifunctional magnetic microsphere and a preparation method thereof, mainly by wrapping polyaniline macromolecules on the surface of magnetic nanoparticles, and successfully connecting fluorescent nanomaterial quantum dots through the functional amino groups at the ends of the polyaniline molecules. Nano-microspheres with magnetic and fluorescent dual functions were obtained. The magnetic fluorescent nano-microspheres of the present invention not only retain the excellent fluorescent properties of quantum dots, but also have superparamagnetism with magnetic particles, and can be collected and separated by simply applying an external magnetic field. The bifunctional fluorescent magnetic particles can be applied in the fields of immunoassay, targeted therapy, cell separation and the like.

Description

technical field [0001] The invention belongs to the technical fields of biomedicine, analytical chemistry, nanometer material science and synthetic chemistry, and specifically relates to a magnetic fluorescent double-function microsphere prepared by connecting fluorescent quantum dots with polyaniline magnetic microspheres. Background technique [0002] With the advancement of medical technology and the increasing requirements for biological materials, nano-magnetic materials with separation function and quantum dots with excellent fluorescent labeling function are two types of materials that have been studied more. In recent years, magnetic composite microspheres have attracted more and more attention. As a functional polymer material with excellent performance, they have shown strong vitality in nuclear magnetic resonance, immobilized enzymes, targeted drugs, cell separation and immune analysis. [0003] Quantum dots (quantumdots, also known as semiconductor nanocrystals),...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/88B01J13/14B82Y20/00B82Y25/00B82Y30/00
Inventor 王秀玲刘勇健周妍董淑玲赵勤
Owner SUZHOU UNIV OF SCI & TECH
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