Bacillus subtilis and application in prevention of rice ustilaginoidea virens
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A technology of Bacillus subtilis and rice smut fungus, applied in the directions of application, bacteria, fungicides, etc., to achieve good control effect and expand the effect of application scope
Inactive Publication Date: 2014-03-12
INST OF PLANT PROTECTION FAAS
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[0007] The object of the present invention is to provide a kind of Bacillus subtilis that has stronger inhibitory activity to rice false smut bacterium and its application in the biological control of rice false smut Inhibition of germination and hyphae growth, development of biocontrol microbial agents for ri
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Embodiment 1
[0044] Embodiment 1: Bacillus subtilis T122F inhibits the germination of conidia of rice false smut
[0045] one, Materials and Methods
[0046] 1. Test medium
[0047] (1) The formula of the improved bacterial fermentation liquid medium for the cultivation of Bacillus subtilis consists of: 40g soybean meal, 20g corn flour, 5g sodium chloride, 2g lactose, 2g calcium carbonate, 3g peptone, 5g yeast powder, ferrous sulfate (7 Water) 0.3g, magnesium sulfate 0.5g, potassium dihydrogen phosphate 0.5g, add water to make up to 1000mL, pH6.5, autoclave at 121℃ for 35min before use.
[0048] (2) The formulation of Wakimoto Tetsu's medium for the isolation of rice smut bacteria consists of: 300g potato, 5g peptone, 15g sucrose, Ca(NO 3 ) 2 4H 2 O 0.5g, Na 2 HPO 4 12H 2 0 2.0g, 16g of agar, add water to make up to 1000mL, autoclave at 121℃ for 20min, add chloramphenicol 50μg / ml to inhibit bacterial growth when the culture medium is cooled to 45℃, mix well, pour into a petri di...
Embodiment 2
[0064] Embodiment 2: Determination of the field control effect of Bacillus subtilis T122F fermented liquid on rice false smut
[0065] one, Materials and Methods
[0066] 1. The Bacillus subtilis T122F fermentation broth for testing
[0067] The composition of the Bacillus subtilis T122F fermentation broth and the culture conditions of the strains are the same as in Example 1.
[0068] 2. The fungicides tested
[0069] 250 g / L tebuconazole aqueous emulsion is provided by Anhui Jixi Nonghua Biotechnology Co., Ltd., 10wt.% difenoconazole water dispersible granule is provided by Anhui Jixi Nonghua Biotechnology Co., Ltd., 2.5wt.% Jinggang mold Su, 10 billion subtilis live spores aqueous solution (wenquning) were provided by Jiangsu Suke Agriculture Co., Ltd.
[0070] 3. Field efficacy determination of Bacillus subtilis T122F fermentation broth and different fungicides on rice false smut
[0071] The experiment was carried out in the common rice smut disease field of Masha...
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Abstract
The invention discloses bacillus subtilis and an application technology thereof in biological prevention of rice ustilaginoidea virens, relating to the technical field of biological prevention of plant diseases. The invention particularly provides bacillus subtilis T122F with obvious bacteriostatic activity on the growth of ustilaginoidea virens. The bacillus subtilis T122F is cultured by an improved bacterial fermentation liquid medium, and the fermentation filtrate can obviously inhibit the conidium germination and hypha expansion of the ustilaginoidea virens, thus the bacillus subtilis T122F has a relatively good prevention effect on the field ustilaginoidea virens, and the prevention effect is remarkably higher than that of the normal dosage of 10% difenoconazole water dispersible granules. The bacillus subtilis and fermentation liquid thereof provided by the invention provide the reference to the preparation of a biological pesticide for preventing rice ustilaginoidea virens; the application range of the bacillus subtilis T122F is widened; the invention is of great significance for guaranteeing the safe production and yield increase of rice of the country and reducing the problems such as environmental pollution and the like caused by mass application of chemical pesticides.
Description
technical field [0001] The invention relates to the technical field of plant disease biological control, more specifically to a Bacillus subtilis ( Bacillus subtilis ) and its application in biological control of rice false smut. Background technique [0002] Rice false smut ( Ustilaginoidea virens (Cooke) Tak) is a fungal disease on the ear of rice, also known as false smut, green smut, commonly known as "harvest disease". It is distributed in rice production areas in more than 40 countries on five continents including China, Japan, the Philippines, India, the United States, Australia, Egypt, and the United Kingdom. Rice false smut is also a major rice disease that has been increasing in my country in the past 10 years. Especially in recent years, the annual damage area of the disease in my country is 5.6-6.4 million hectares, and the occurrence and damage area in a pandemic year can reach 10 million hectares. The diseased ear rate of the general field is about 3%~5%,...
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