Coaxial three-layer high-activity bio-enzyme-embedded electrostatic spinning nano-fiber membrane and preparation method thereof

A nanofiber membrane and electrospinning technology, which is applied in spinning solution preparation, fiber treatment, rayon manufacturing, etc., can solve the problems of low enzyme reusability, limited enzyme load, poor contact between enzyme and substrate, etc. Achieve the effects of improving catalytic efficiency and reusability, promoting full contact, and reducing mass transfer resistance

Inactive Publication Date: 2014-03-26
DALIAN OCEAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of this method can only fix the enzyme monolayer on the surface of the fiber membrane, the enzyme load is limited, and the enzyme falls off during the reaction process, resulting in low enzyme reusability, although the enzyme load and The stability of the immobilized enzyme, but the enzyme activity will be greatly affected
The second method is to ble

Method used

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  • Coaxial three-layer high-activity bio-enzyme-embedded electrostatic spinning nano-fiber membrane and preparation method thereof
  • Coaxial three-layer high-activity bio-enzyme-embedded electrostatic spinning nano-fiber membrane and preparation method thereof
  • Coaxial three-layer high-activity bio-enzyme-embedded electrostatic spinning nano-fiber membrane and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] a. Disperse 1.0 g of polyvinyl alcohol particles into 9 ml of deionized water, stir magnetically for 1 h at room temperature until fully swollen; the temperature rises to 70 °C, continue to stir until the solution is clear and transparent, slowly add 0.01 g of KCl to the above solution, stir magnetically to complete dissolution;

[0024] b. Dissolve 0.2 mg of active peroxidase in 0.5 ml of phosphate buffer solution with a pH value of 7.0, shake until it is completely dissolved to obtain a peroxidase dispersion; add the above peroxidase dispersion to 12 ml In the polyvinyl alcohol solution with a concentration of 11% (wt), shake well to uniformly disperse, and store at 4 °C for later use;

[0025] c. Dissolve 1.0 g of polystyrene particles in 9 ml of tetrahydrofuran solvent, and stir magnetically for 1 h at room temperature to form a homogeneous, stable and viscous sol;

[0026] d. The solution obtained in steps a to c is poured into a three-nozzle coaxial electrospinni...

Embodiment 2

[0029] a. Disperse 1.0 g of polyacrylic acid particles into 6 ml of deionized water, stir magnetically for 1 h at room temperature until fully swollen, raise the temperature to 70 °C, continue to stir until the solution is clear and transparent, slowly add 0.01 g of CaCl to the above solution 2 , magnetic stirring until completely dissolved;

[0030] b. Dissolve 0.2 mg of active lipase in 0.5 ml of a phosphate buffer solution with a pH value of 7.0, shake and shake until it is completely dissolved to obtain a lipase dispersion; add the above lipase dispersion to 10 ml with a concentration of 10% (wt ) in the polyvinyl acid solution, shake well until uniformly dispersed, and store at 4°C for later use;

[0031] c. Dissolve 1.0 g of polymethyl methacrylate particles in 3.5 ml of tetrahydrofuran solvent, and stir magnetically for 1 h at room temperature to form a homogeneous, stable and viscous sol;

[0032] d. The solution obtained in steps a to c is poured into the three-nozzl...

Embodiment 3

[0035] a. Disperse 1.0 g of polyacrylamide particles into 19 ml of deionized water, stir magnetically at room temperature for 1 hour until the solution is clear and transparent, slowly add 0.2 g of ethylenediaminetetraacetic acid (EDTA) to the above solution, and stir magnetically until completely dissolved;

[0036] b. Dissolve 0.2 mg of active urease in 0.6 ml of phosphate buffer solution with a pH value of 7.0, oscillate until completely dissolved to obtain a urease dispersion, add the above urease dispersion to 10ml with a concentration of 10% (wt ) in polyacrylamide solution, shake well until evenly dispersed, and store at 4°C until use;

[0037] c. Dissolve 1.0 g of polyacrylonitrile particles in 12 ml of dimethylformamide solvent, and stir magnetically at room temperature for 1 hour until a uniform, stable, and viscous sol is formed;

[0038] d. Pouring the solutions obtained in steps a to c into the three-nozzle coaxial electrospinning device respectively, using the so...

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Abstract

The invention discloses a coaxial three-layer high-activity bio-enzyme-embedded electrostatic spinning nano-fiber membrane and a preparation method thereof. The coaxial three-way electrostatic spinning technology is used to allow bio-enzyme to be embedded into the sandwich layer of slow release activating agent and porous polymer so as to form the porous nano-fiber membrane. The nano-fiber membrane of a sandwich structure sequentially comprises a slow release activating agent layer (1), an immobilized bio-enzyme layer (2) and a porous polymer layer (3) from inside to outside. By building the structure with communicating ducts, immobilization amount of enzyme is increased, sufficient contact of the enzyme and a substrate is promoted, mass transfer resistance between the enzyme and the substrate is lowered, catalytic efficiency and reusability of the enzyme are increased effectively, environmental sensitivity of the bio-enzyme is lowered, and lasting stability of the bio-enzyme when being applied in complex environments is increased.

Description

technical field [0001] The invention relates to an electrospinning nanofiber membrane and a preparation method, in particular to a coaxial three-layer high-activity embedded biological enzyme with the characteristics of high activity, long-lasting stability and strong environmental broad-spectrum. Electrospinning nanofiber membrane and preparation method thereof. Background technique [0002] Biological enzyme immobilization technology is a technology that uses chemical or physical methods to locate free cells in a defined area to keep them active and can be reused. It has the advantages of strong, less loss of biological enzymes. The existing biological enzyme immobilization methods include adsorption method, covalent method, cross-linking method and embedding method. Adsorption method is the simplest and most direct enzyme immobilization method. It has the advantages of high recovery rate, easy availability and regeneration of the carrier, etc., but the binding force bet...

Claims

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Application Information

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IPC IPC(8): D01D5/00D04H1/728D04H1/4382D01D1/02D01F1/10
Inventor 潘超
Owner DALIAN OCEAN UNIV
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